Tetrahydrobiopterin metabolism

Abstract

A technique for the measurement of biopterin using HPLC has been developed and compared against the established Crithidia fasciculata assay. The two assays give a similar reading when samples are unoxidised but after acid/iodine oxidation Crithidia reads higher, probably due to the presence of other pterins.

Using HPLC as a measuring technique an oral dose of phenylalanine has been found to elevate the level of biopterin derivatives in the blood whilst tyrosine had no effect...

Date of Award	1984
Original language	English
Awarding Institution	Aston University