Abstract
The composition of the cell wall of Streptococcus faecalis was examined for the presence of specialised structures or components responsible forattachment to host endocardial tissue in infective endocarditis (IE).
Analysis of cells by sodium dodecylsulphate-polyacrylamide gel
electrophoresis (SDS-PAGE), crossed immunoelectrophoresis (CIE) and
immunoblotting revealed that the protein and antigenic profiles of cells grown in complex laboratory medium were different to those of cells grown in conditions approximating to the in vivo environment. The antigenic
pattern of S. faecalis was more complex after growth in brain-heart
infusion than after growth in serum. Growth in serum resulted in expression of three major protein antigens, 73, 40 and 37K, which were characteristic of S. faecalis species and were absent from other streptococcal species and
Staphylococcus aureus...
| Date of Award | Sept 1987 |
|---|---|
| Original language | English |
| Awarding Institution |
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Keywords
- antigenic composition
- Streptococcus faecalis
- ineffective endocarditis