Acute and long-term effects of metformin on the function and insulin secretory responsiveness of clonal β-cells

Joan M. McKiney, Nigel Irwin, Peter R. Flatt, Clifford J. Bailey, Neville H. McClenaghan

Research output: Contribution to journalArticlepeer-review

Abstract

Functional effects of acute and prolonged (48 h) exposure to the biguanide drug metformin were examined in the clonal pancreatic ß-cell line, BRIN-BD11. Effects of metformin on prolonged exposure to excessive increased concentrations of glucose and palmitic acid were also assessed. In acute 20-min incubations, 12.5-50 µm metformin did not alter basal (1.1 mm glucose) or glucose-stimulated (16.7 mm glucose) insulin secretion. However, higher concentrations of metformin (100-1000 µm) increased (1.3-1.5-fold; p
Original languageEnglish
Pages (from-to)1451-1459
Number of pages9
JournalBiological Chemistry
Volume391
Issue number12
DOIs
Publication statusPublished - Dec 2010

Keywords

  • animals
  • calcium
  • cell survival
  • cultured cells
  • drug dose-response relationship
  • gene expression
  • glucose
  • hypoglycemic agents
  • insulin
  • insulin-secreting cells
  • islets of Langerhans
  • metformin
  • palmitic acid
  • rats
  • time factors

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