Attenuation of depression of muscle protein synthesis induced by lipopolysaccharide, tumor necrosis factor, and angiotensin II by β-hydroxy-β-methylbutyrate

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  • Helen L. Eley
  • Steven T. Russell
  • Michael J. Tisdale

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β-Hydroxy-β-methylbutyrate (HMB; 50 μM) has been shown to attenuate the depression in protein synthesis in murine myotubes in response to lipopolysaccharide (LPS), tumor necrosis factor-α (TNF-α) with or without interferon-γ (IFN-γ), and angiotensin II (ANG II). The mechanism for the depression of protein synthesis by all three agents was the same and was attributed to activation of double-stranded RNA-dependent protein kinase (PKR) with the subsequent phosphorylation of eukaryotic initiation factor 2 (eIF2) on the α-subunit as well as increased phosphorylation of the elongation factor (eEF2). Myotubes expressing a catalytically inactive PKR variant, PKRΔ6, showed no depression of protein synthesis in response to either LPS or TNF-α, confirming the importance of PKR in this process. There was no effect of any of the agents on phosphorylation of mammalian target of rapamycin (mTOR) or initiation factor 4E-binding protein (4E-BP1), and thus no change in the amount of eIF4E bound to 4E-BP1 or the concentration of the active eIF4E·eIF4G complex. HMB attenuated phosphorylation of eEF2, possibly by increasing phosphorylation of mTOR, and also attenuated phosphorylation of eIF2α by preventing activation of PKR. These results suggest that HMB may be effective in attenuating muscle atrophy in a range of catabolic conditions.


Original languageEnglish
Pages (from-to)E1409-E1416
Number of pages8
JournalAmerican Journal of Physiology
Issue number6
Publication statusPublished - Dec 2008


  • Eukaryotic initiation factor 2α

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Employable Graduates; Exploitable Research

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