c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE

Alan D. Goddard; Julie M. Stevens; Feng Rao; Despoina A.I. Mavridou; Weelee Chan; David J. Richardson; James W.A. Allen; Stuart J. Ferguson

doi:10.1074/jbc.M110.133421

c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE

Alan D. Goddard, Julie M. Stevens, Feng Rao, Despoina A.I. Mavridou, Weelee Chan, David J. Richardson, James W.A. Allen, Stuart J. Ferguson

College of Health and Life Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The Ccm cytochrome c maturation System I catalyzes covalent attachment of heme to apocytochromes c in many bacterial species and some mitochondria. A covalent, but transient, bond between heme and a conserved histidine in CcmE along with an interaction between CcmH and the apocytochrome have been previously indicated as core aspects of the Ccm system. Here, we show that in the Ccm system from Desulfovibrio desulfuricans, no CcmH is required, and the holo-CcmE covalent bond occurs via a cysteine residue. These observations call for reconsideration of the accepted models of System I-mediated c-type cytochrome biogenesis.

Original language	English
Pages (from-to)	22882-22889
Number of pages	8
Journal	Journal of Biological Chemistry
Volume	285
Issue number	30
Early online date	13 May 2010
DOIs	https://doi.org/10.1074/jbc.M110.133421
Publication status	Published - 23 Jul 2010

Bibliographical note

© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

Errata: Goddard, A. D., Stevens, J. M., Rao, F., Mavridou, D. A. I., Chan, W., Richardson, D. J., ... Ferguson, S. J. (2011). c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE. Journal of Biological Chemistry, 286(33), 29442. DOI: 10.1074/jbc.A110.133421

Keywords

chaperone chaperonin
cytochrome c
cytochromes
heme
post-translational modification
protein assembly
cytochrome c biogenesis
cytochrome c maturation
heme chaperone

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10.1074/jbc.M110.133421

c-Type Cytochrome Biogenesis Can Occur via a Natural Ccm System
© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
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title = "c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE",

abstract = "The Ccm cytochrome c maturation System I catalyzes covalent attachment of heme to apocytochromes c in many bacterial species and some mitochondria. A covalent, but transient, bond between heme and a conserved histidine in CcmE along with an interaction between CcmH and the apocytochrome have been previously indicated as core aspects of the Ccm system. Here, we show that in the Ccm system from Desulfovibrio desulfuricans, no CcmH is required, and the holo-CcmE covalent bond occurs via a cysteine residue. These observations call for reconsideration of the accepted models of System I-mediated c-type cytochrome biogenesis.",

keywords = "chaperone chaperonin, cytochrome c, cytochromes, heme, post-translational modification, protein assembly, cytochrome c biogenesis, cytochrome c maturation , heme chaperone",

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AU - Mavridou, Despoina A.I.

AU - Chan, Weelee

AU - Richardson, David J.

AU - Allen, James W.A.

AU - Ferguson, Stuart J.

N1 - © 2010 by The American Society for Biochemistry and Molecular Biology, Inc. Errata: Goddard, A. D., Stevens, J. M., Rao, F., Mavridou, D. A. I., Chan, W., Richardson, D. J., ... Ferguson, S. J. (2011). c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE. Journal of Biological Chemistry, 286(33), 29442. DOI: 10.1074/jbc.A110.133421

PY - 2010/7/23

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N2 - The Ccm cytochrome c maturation System I catalyzes covalent attachment of heme to apocytochromes c in many bacterial species and some mitochondria. A covalent, but transient, bond between heme and a conserved histidine in CcmE along with an interaction between CcmH and the apocytochrome have been previously indicated as core aspects of the Ccm system. Here, we show that in the Ccm system from Desulfovibrio desulfuricans, no CcmH is required, and the holo-CcmE covalent bond occurs via a cysteine residue. These observations call for reconsideration of the accepted models of System I-mediated c-type cytochrome biogenesis.

AB - The Ccm cytochrome c maturation System I catalyzes covalent attachment of heme to apocytochromes c in many bacterial species and some mitochondria. A covalent, but transient, bond between heme and a conserved histidine in CcmE along with an interaction between CcmH and the apocytochrome have been previously indicated as core aspects of the Ccm system. Here, we show that in the Ccm system from Desulfovibrio desulfuricans, no CcmH is required, and the holo-CcmE covalent bond occurs via a cysteine residue. These observations call for reconsideration of the accepted models of System I-mediated c-type cytochrome biogenesis.

KW - chaperone chaperonin

KW - cytochrome c

KW - cytochromes

KW - heme

KW - post-translational modification

KW - protein assembly

KW - cytochrome c biogenesis

KW - cytochrome c maturation

KW - heme chaperone

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c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE

Abstract

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Keywords

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