Developing solid particulate vaccine adjuvants: surface bound antigen favouring a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity

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This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-co-glycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.



Original languageEnglish
Pages (from-to)268-278
Number of pages11
JournalCurrent Drug Delivery
Issue number6
Early online date4 Feb 2013
Publication statusPublished - 2013

Bibliographic note

This study was funded by the European Commission 459 (contract no. LSHP-CT-2003-503367). This work was also part funded by NewTBVAC (contract no. 460 HEALTH-F3-2009-241745). NewTBVAC has been made possible by contributions from the European 461 Commission.


  • adjuvant, DDA, ESEM, microspheres, PLGA, subunit vaccine

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