Developing solid particulate vaccine adjuvants: surface bound antigen favouring a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity

Research output: Contribution to journalArticle

View graph of relations Save citation

Open

Authors

Research units

Abstract

This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-co-glycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.

Documents

Details

Original languageEnglish
Pages (from-to)268-278
Number of pages11
JournalCurrent Drug Delivery
Volume11
Issue number6
Early online date4 Feb 2013
DOIs
Publication statusPublished - 2013

Bibliographic note

This study was funded by the European Commission 459 (contract no. LSHP-CT-2003-503367). This work was also part funded by NewTBVAC (contract no. 460 HEALTH-F3-2009-241745). NewTBVAC has been made possible by contributions from the European 461 Commission.

    Keywords

  • adjuvant, DDA, ESEM, microspheres, PLGA, subunit vaccine

Download statistics

No data available

Employable Graduates; Exploitable Research

Copy the text from this field...