DNA-templated protein arrays for single-molecule imaging

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Abstract

Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show that self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules.

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Original languageEnglish
Pages (from-to)657-660
Number of pages4
JournalNano Letters
Volume11
Issue number2
Early online date10 Jan 2011
DOIs
Publication statusPublished - 9 Feb 2011

    Keywords

  • DNA templates, G-protein-coupled membrane receptor, nanoaffinity, nanostructure, single-particle electron cryomicrosopy

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