Interfacing low-energy SAW nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples

Karina Tveen Jensen; F. Gesellchen; Rab Wilson; Corinne Spickett; Jonathan M. Cooper; Andrew Pitt

doi:10.1038/srep09736

Interfacing low-energy SAW nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples

Karina Tveen Jensen, F. Gesellchen, Rab Wilson, Corinne Spickett, Jonathan M. Cooper, Andrew Pitt^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Soft ionization methods for the introduction of labile biomolecules into a mass spectrometer are of fundamental importance to biomolecular analysis. Previously, electrospray ionization (ESI) and matrix assisted laser desorption-ionization (MALDI) have been the main ionization methods used. Surface acoustic wave nebulization (SAWN) is a new technique that has been demonstrated to deposit less energy into ions upon ion formation and transfer for detection than other methods for sample introduction into a mass spectrometer (MS). Here we report the optimization and use of SAWN as a nebulization technique for the introduction of samples from a low flow of liquid, and the interfacing of SAWN with liquid chromatographic separation (LC) for the analysis of a protein digest. This demonstrates that SAWN can be a viable, low-energy alternative to ESI for the LC-MS analysis of proteomic samples.

Original language	English
Article number	9736
Number of pages	5
Journal	Scientific Reports
Volume	5
DOIs	https://doi.org/10.1038/srep09736
Publication status	Published - 15 May 2015

Bibliographical note

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission fromthe license holder in order to reproduce thematerial. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Funding: EPSRC (EP/I017887/1; P/K027611/1); Aston University; ERC advanced investigator award (340117 – Biophonics).

Supplementary information accompanies this paper at http://www.nature.com/
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10.1038/srep09736

Interfacing low-energy SAW nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission fromthe license holder in order to reproduce thematerial. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Final published version, 1.02 MBLicence: CC BY 3.0

http://www.nature.com/srep/2015/150515/srep09736/full/srep09736.html

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title = "Interfacing low-energy SAW nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples",

abstract = "Soft ionization methods for the introduction of labile biomolecules into a mass spectrometer are of fundamental importance to biomolecular analysis. Previously, electrospray ionization (ESI) and matrix assisted laser desorption-ionization (MALDI) have been the main ionization methods used. Surface acoustic wave nebulization (SAWN) is a new technique that has been demonstrated to deposit less energy into ions upon ion formation and transfer for detection than other methods for sample introduction into a mass spectrometer (MS). Here we report the optimization and use of SAWN as a nebulization technique for the introduction of samples from a low flow of liquid, and the interfacing of SAWN with liquid chromatographic separation (LC) for the analysis of a protein digest. This demonstrates that SAWN can be a viable, low-energy alternative to ESI for the LC-MS analysis of proteomic samples.",

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AB - Soft ionization methods for the introduction of labile biomolecules into a mass spectrometer are of fundamental importance to biomolecular analysis. Previously, electrospray ionization (ESI) and matrix assisted laser desorption-ionization (MALDI) have been the main ionization methods used. Surface acoustic wave nebulization (SAWN) is a new technique that has been demonstrated to deposit less energy into ions upon ion formation and transfer for detection than other methods for sample introduction into a mass spectrometer (MS). Here we report the optimization and use of SAWN as a nebulization technique for the introduction of samples from a low flow of liquid, and the interfacing of SAWN with liquid chromatographic separation (LC) for the analysis of a protein digest. This demonstrates that SAWN can be a viable, low-energy alternative to ESI for the LC-MS analysis of proteomic samples.

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Interfacing low-energy SAW nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples

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