Technical challenges of working with extracellular vesicles

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Authors

  • Marcel I. Ramirez
  • Maria G. Amorim
  • Catarina Gadelha
  • Joshua A. Welsh
  • Vanessa M. Freitas
  • Muhammad Nawaz
  • Naveed Akbar
  • Yvonne Couch
  • Laura Makin
  • Fiona Cooke
  • Andre L. Vettore
  • Patricia X. Batista
  • Roberta Freezor
  • Julia A. Pezuk
  • Lívia Rosa-Fernandes
  • Ana Claudia O. Carreira
  • Laura Jacobs
  • Israel T. Silva
  • Gillian Coakley
  • Diana N. Nunes
  • Dave Carter
  • Giuseppe Palmisano
  • Emmanuel Dias-Neto

Research units

Abstract

Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

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Details

Original languageEnglish
Pages (from-to)881-906
Number of pages26
JournalNanoscale
Volume10
Issue number3
Early online date13 Dec 2017
DOIs
Publication statusPublished - 13 Dec 2017

Bibliographic note

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

DOI

Employable Graduates; Exploitable Research

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