The visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticles

David Y.S. Chau; Naing L. Tint; Russell J Collighan; Martin Griffin; Harminder S. Dua; Kevin M. Shakesheff; Felicity R.A.J. Rose

doi:10.1136/bjo.2009.163642

The visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticles

David Y.S. Chau, Naing L. Tint, Russell J Collighan, Martin Griffin, Harminder S. Dua, Kevin M. Shakesheff, Felicity R.A.J. Rose

Research output: Contribution to journal › Article › peer-review

Abstract

AIMS To demonstrate the potential use of in vitro poly(lactic-co-glycolic acid) (PLGA) microparticles in comparison with triamcinolone suspension to aid visualisation of vitreous during anterior and posterior vitrectomy. METHODS PLGA microparticles (diameter 10-60 microm) were fabricated using single and/or double emulsion technique(s) and used untreated or following the surface adsorption of a protein (transglutaminase). Particle size, shape, morphology and surface topography were assessed using scanning electron microscopy (SEM) and compared with a standard triamcinolone suspension. The efficacy of these microparticles to enhance visualisation of vitreous against the triamcinolone suspension was assessed using an in vitro set-up exploiting porcine vitreous. RESULTS Unmodified PLGA microparticles failed to adequately adhere to porcine vitreous and were readily washed out by irrigation. In contrast, modified transglutaminase-coated PLGA microparticles demonstrated a significant improvement in adhesiveness and were comparable to a triamcinolone suspension in their ability to enhance the visualisation of vitreous. This adhesive behaviour also demonstrated selectivity by not binding to the corneal endothelium. CONCLUSION The use of transglutaminase-modified biodegradable PLGA microparticles represents a novel method of visualising vitreous and aiding vitrectomy. This method may provide a distinct alternative for the visualisation of vitreous whilst eliminating the pharmacological effects of triamcinolone acetonide suspension.

Original language	English
Pages (from-to)	648-653
Number of pages	6
Journal	British Journal of Ophthalmology
Volume	94
Issue number	5
DOIs	https://doi.org/10.1136/bjo.2009.163642
Publication status	Published - 2010

Bibliographical note

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license. See: http://creativecommons.org/licenses/by-nc/2.0/ and http://creativecommons.org/licenses/by-nc/2.0/legalcode.

Keywords

Adhesiveness
Glycolates
Humans
Lactic Acid
Microscopy, Electron, Scanning
Microspheres
Particle Size
Polyglycolic Acid
Triamcinolone
Vitrectomy
Vitreous Body

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10.1136/bjo.2009.163642

The visualisation of vitreous
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license. See: http://creativecommons.org/licenses/by-nc/2.0/ and http://creativecommons.org/licenses/by-nc/2.0/legalcode.
Final published version, 968 KBLicence: CC BY-NC 3.0

Cite this

@article{00d2e50b731849c3a6fc437f8fd3e827,

title = "The visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticles",

abstract = "AIMS To demonstrate the potential use of in vitro poly(lactic-co-glycolic acid) (PLGA) microparticles in comparison with triamcinolone suspension to aid visualisation of vitreous during anterior and posterior vitrectomy. METHODS PLGA microparticles (diameter 10-60 microm) were fabricated using single and/or double emulsion technique(s) and used untreated or following the surface adsorption of a protein (transglutaminase). Particle size, shape, morphology and surface topography were assessed using scanning electron microscopy (SEM) and compared with a standard triamcinolone suspension. The efficacy of these microparticles to enhance visualisation of vitreous against the triamcinolone suspension was assessed using an in vitro set-up exploiting porcine vitreous. RESULTS Unmodified PLGA microparticles failed to adequately adhere to porcine vitreous and were readily washed out by irrigation. In contrast, modified transglutaminase-coated PLGA microparticles demonstrated a significant improvement in adhesiveness and were comparable to a triamcinolone suspension in their ability to enhance the visualisation of vitreous. This adhesive behaviour also demonstrated selectivity by not binding to the corneal endothelium. CONCLUSION The use of transglutaminase-modified biodegradable PLGA microparticles represents a novel method of visualising vitreous and aiding vitrectomy. This method may provide a distinct alternative for the visualisation of vitreous whilst eliminating the pharmacological effects of triamcinolone acetonide suspension.",

keywords = "Adhesiveness, Glycolates, Humans, Lactic Acid, Microscopy, Electron, Scanning, Microspheres, Particle Size, Polyglycolic Acid, Triamcinolone, Vitrectomy, Vitreous Body",

author = "Chau, {David Y.S.} and Tint, {Naing L.} and Collighan, {Russell J} and Martin Griffin and Dua, {Harminder S.} and Shakesheff, {Kevin M.} and Rose, {Felicity R.A.J.}",

note = "This is an open-access article distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license. See: http://creativecommons.org/licenses/by-nc/2.0/ and http://creativecommons.org/licenses/by-nc/2.0/legalcode. ",

year = "2010",

doi = "10.1136/bjo.2009.163642",

language = "English",

volume = "94",

pages = "648--653",

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T1 - The visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticles

AU - Chau, David Y.S.

AU - Tint, Naing L.

AU - Collighan, Russell J

AU - Griffin, Martin

AU - Dua, Harminder S.

AU - Shakesheff, Kevin M.

AU - Rose, Felicity R.A.J.

N1 - This is an open-access article distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license. See: http://creativecommons.org/licenses/by-nc/2.0/ and http://creativecommons.org/licenses/by-nc/2.0/legalcode.

PY - 2010

Y1 - 2010

N2 - AIMS To demonstrate the potential use of in vitro poly(lactic-co-glycolic acid) (PLGA) microparticles in comparison with triamcinolone suspension to aid visualisation of vitreous during anterior and posterior vitrectomy. METHODS PLGA microparticles (diameter 10-60 microm) were fabricated using single and/or double emulsion technique(s) and used untreated or following the surface adsorption of a protein (transglutaminase). Particle size, shape, morphology and surface topography were assessed using scanning electron microscopy (SEM) and compared with a standard triamcinolone suspension. The efficacy of these microparticles to enhance visualisation of vitreous against the triamcinolone suspension was assessed using an in vitro set-up exploiting porcine vitreous. RESULTS Unmodified PLGA microparticles failed to adequately adhere to porcine vitreous and were readily washed out by irrigation. In contrast, modified transglutaminase-coated PLGA microparticles demonstrated a significant improvement in adhesiveness and were comparable to a triamcinolone suspension in their ability to enhance the visualisation of vitreous. This adhesive behaviour also demonstrated selectivity by not binding to the corneal endothelium. CONCLUSION The use of transglutaminase-modified biodegradable PLGA microparticles represents a novel method of visualising vitreous and aiding vitrectomy. This method may provide a distinct alternative for the visualisation of vitreous whilst eliminating the pharmacological effects of triamcinolone acetonide suspension.

AB - AIMS To demonstrate the potential use of in vitro poly(lactic-co-glycolic acid) (PLGA) microparticles in comparison with triamcinolone suspension to aid visualisation of vitreous during anterior and posterior vitrectomy. METHODS PLGA microparticles (diameter 10-60 microm) were fabricated using single and/or double emulsion technique(s) and used untreated or following the surface adsorption of a protein (transglutaminase). Particle size, shape, morphology and surface topography were assessed using scanning electron microscopy (SEM) and compared with a standard triamcinolone suspension. The efficacy of these microparticles to enhance visualisation of vitreous against the triamcinolone suspension was assessed using an in vitro set-up exploiting porcine vitreous. RESULTS Unmodified PLGA microparticles failed to adequately adhere to porcine vitreous and were readily washed out by irrigation. In contrast, modified transglutaminase-coated PLGA microparticles demonstrated a significant improvement in adhesiveness and were comparable to a triamcinolone suspension in their ability to enhance the visualisation of vitreous. This adhesive behaviour also demonstrated selectivity by not binding to the corneal endothelium. CONCLUSION The use of transglutaminase-modified biodegradable PLGA microparticles represents a novel method of visualising vitreous and aiding vitrectomy. This method may provide a distinct alternative for the visualisation of vitreous whilst eliminating the pharmacological effects of triamcinolone acetonide suspension.

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KW - Glycolates

KW - Humans

KW - Lactic Acid

KW - Microscopy, Electron, Scanning

KW - Microspheres

KW - Particle Size

KW - Polyglycolic Acid

KW - Triamcinolone

KW - Vitrectomy

KW - Vitreous Body

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DO - 10.1136/bjo.2009.163642

M3 - Article

C2 - 20447968

SN - 1468-2079

VL - 94

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EP - 653

JO - British Journal of Ophthalmology

JF - British Journal of Ophthalmology

IS - 5

ER -

The visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticles

Abstract

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Keywords

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