The yin and yang of solubilization and stabilization for wild-type and full-length membrane protein

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Abstract

Membrane proteins (MP) are stable in their native lipid environment. To enable structural and functional investigations, MP need to be extracted from the membrane. This is a critical step that represents the main obstacle for MP biochemistry and structural biology. General guidelines and rules for membrane protein solubilization remain difficult to establish. This review aims to provide the reader with a comprehensive overview of the general concepts of MP solubilization and stabilization as well as recent advances in detergents innovation. Understanding how solubilization and stabilization are intimately linked is key to facilitate MP isolation toward fundamental structural and functional research as well as drug discovery applications. How to manage the tour de force of destabilizing the lipid bilayer and stabilizing MP at the same time is the holy grail of successful isolation and investigation of such a delicate and fascinating class of proteins.

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  • The yin and yang of solubilization and stabilization for wild-type

    Rights statement: © 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

    Accepted author manuscript, 124 KB, Word-document

    Embargo ends: 23/02/19

    Licence: CC BY-NC-ND Show licence

Details

Original languageEnglish
Pages (from-to)118-125
JournalMethods
Volume147
Early online date23 Feb 2018
DOIs
StatePublished - 1 Sep 2018

Bibliographic note

© 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

    Keywords

  • Membrane proteins, Detergent, Surfactant, Solubilization, Stabilization

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