Abundant Active Ingredients of Green Tea Regulate Glucose Metabolism in 3T3-L1 Cell Line

Sattar J. J. Al-shaeli, Ali M. Ethaeb, James E Brown

Research output: Contribution to journalArticle

Abstract

Adipose tissue has a crucial role involving in regulation of glucose and lipid metabolism dysfunction of adipose tissue can disrupted glucose and lipid regulation process which both are a hill marks of obesity, insulin resistance and type 2 diabetes (T2D). Recently, green tea and its abundant active compounds showed several potential health benefits including amelioration this impairment, however, the mechanism of this effect is not yet fully understood. Therefore, the effect of epigallocatechin gallate (EGCG), epicatechin (EC), and epicatechin gallate (ECG) on glucose uptake and utilisation in 3T3-L1 (adipocyte), and the possible mechanism of this impact were determined. Glucose uptake in the presence of adenosine 5’-monophosphate-activated protein kinase (AMPK) and protein kinase B (Akt) inhibitors, triglyceride and glycerol released, cell viability, and metabolic gene expression were investigated. Selected green tea compounds significantly increased glucose uptake without alteration cell viability, and this biological changing was suppressed by additive Akt inhibitor molecule. A remarkable reduction in cellular triglyceride and glycerol released were seen, which suggesting the compounds suppressed adipogenesis and lipolysis, however measurement of adipogenic and lipolysis gene expression showed no significant alteration. In conclusion, these active compounds of green tea could regulate glucose and lipid metabolism through activating phosphoinositide 3-kinase (PI3K)/Akt possibly its downstream signalling, and therefore could be a potential anti-obesity and anti-diabetic agent.
Original languageEnglish
Pages (from-to)738-744
Number of pages7
JournalIndian Journal of Forensic Medicine & Toxicology
Volume13
Issue number4
Early online date27 Nov 2019
DOIs
Publication statusE-pub ahead of print - 27 Nov 2019

Fingerprint

3T3-L1 Cells
Tea
Glucose
Cell Line
Lipolysis
Lipid Metabolism
Glycerol
Adipose Tissue
Cell Survival
Triglycerides
Anti-Obesity Agents
Gene Expression
Adipogenesis
Proto-Oncogene Proteins c-akt
1-Phosphatidylinositol 4-Kinase
Insurance Benefits
Adenosine Monophosphate
Adipocytes
Protein Kinases
Type 2 Diabetes Mellitus

Keywords

  • 3T3-L1
  • AML12
  • C2C12
  • Glucose metabolism
  • Green tea
  • Lipid metabolism

Cite this

Al-shaeli, S. J. J., Ethaeb, A. M., & Brown, J. E. (2019). Abundant Active Ingredients of Green Tea Regulate Glucose Metabolism in 3T3-L1 Cell Line. Indian Journal of Forensic Medicine & Toxicology, 13(4), 738-744. https://doi.org/10.5958/0973-9130.2019.00381.5
Al-shaeli, Sattar J. J. ; Ethaeb, Ali M. ; Brown, James E. / Abundant Active Ingredients of Green Tea Regulate Glucose Metabolism in 3T3-L1 Cell Line. In: Indian Journal of Forensic Medicine & Toxicology. 2019 ; Vol. 13, No. 4. pp. 738-744.
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Al-shaeli, SJJ, Ethaeb, AM & Brown, JE 2019, 'Abundant Active Ingredients of Green Tea Regulate Glucose Metabolism in 3T3-L1 Cell Line', Indian Journal of Forensic Medicine & Toxicology, vol. 13, no. 4, pp. 738-744. https://doi.org/10.5958/0973-9130.2019.00381.5

Abundant Active Ingredients of Green Tea Regulate Glucose Metabolism in 3T3-L1 Cell Line. / Al-shaeli, Sattar J. J.; Ethaeb, Ali M.; Brown, James E.

In: Indian Journal of Forensic Medicine & Toxicology, Vol. 13, No. 4, 27.11.2019, p. 738-744.

Research output: Contribution to journalArticle

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AU - Ethaeb, Ali M.

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AB - Adipose tissue has a crucial role involving in regulation of glucose and lipid metabolism dysfunction of adipose tissue can disrupted glucose and lipid regulation process which both are a hill marks of obesity, insulin resistance and type 2 diabetes (T2D). Recently, green tea and its abundant active compounds showed several potential health benefits including amelioration this impairment, however, the mechanism of this effect is not yet fully understood. Therefore, the effect of epigallocatechin gallate (EGCG), epicatechin (EC), and epicatechin gallate (ECG) on glucose uptake and utilisation in 3T3-L1 (adipocyte), and the possible mechanism of this impact were determined. Glucose uptake in the presence of adenosine 5’-monophosphate-activated protein kinase (AMPK) and protein kinase B (Akt) inhibitors, triglyceride and glycerol released, cell viability, and metabolic gene expression were investigated. Selected green tea compounds significantly increased glucose uptake without alteration cell viability, and this biological changing was suppressed by additive Akt inhibitor molecule. A remarkable reduction in cellular triglyceride and glycerol released were seen, which suggesting the compounds suppressed adipogenesis and lipolysis, however measurement of adipogenic and lipolysis gene expression showed no significant alteration. In conclusion, these active compounds of green tea could regulate glucose and lipid metabolism through activating phosphoinositide 3-kinase (PI3K)/Akt possibly its downstream signalling, and therefore could be a potential anti-obesity and anti-diabetic agent.

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Al-shaeli SJJ, Ethaeb AM, Brown JE. Abundant Active Ingredients of Green Tea Regulate Glucose Metabolism in 3T3-L1 Cell Line. Indian Journal of Forensic Medicine & Toxicology. 2019 Nov 27;13(4):738-744. https://doi.org/10.5958/0973-9130.2019.00381.5