Activation of the neutrophil respiratory burst by plasma from periodontitis patients is mediated by pro-inflammatory cytokines

Irundika H K Dias, John B. Matthews, Iain L.C. Chapple, Helen J. Wright, Christopher R Dunston, Helen R Griffiths

Research output: Contribution to journalArticle

Abstract

Aim: To determine the effect of periodontitis patients' plasma on the neutrophil oxidative burst and the role of albumin, immunoglobulins (Igs) and cytokines.
Materials and Methods: Plasma was collected from chronic periodontitis patients (n=11) and periodontally healthy controls (n=11) and used with/without depletion of albumin and Ig or antibody neutralization of IL-8, GM-CSF or IFN-a to prime/stimulate peripheral blood neutrophils, isolated from healthy volunteers. The respiratory burst was measured by lucigenin-dependent chemiluminescence. Plasma cytokine levels were determined by ELISA.
Results: Plasmas from patients were significantly more effective in both directly stimulating neutrophil superoxide production and priming for subsequent formyl-met-leu-phe (fMLP)-stimulated superoxide production than plasmas from healthy controls (p<0.05). This difference was maintained after depletion of albumin and Ig. Plasma from patients contained higher mean levels of IL-8, GM-CSF and IFN-a. Individual neutralizing antibodies against IL-8, GM-CSF or IFN-a inhibited the direct stimulatory effect of patients' plasma, whereas the ability to prime for fMLP-stimulated superoxide production was only inhibited by neutralization of IFN-a. The stimulating and priming effects of control plasma were unaffected by antibody neutralization.
Conclusions: This study demonstrates that plasma cytokines may have a role in inducing the hyperactive (IL-8, GM-CSF, IFN-a) and hyper-reactive (IFN-a) neutrophil phenotype seen in periodontitis patients.
Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Clinical Periodontology
Volume38
Issue number1
Early online date21 Oct 2010
DOIs
Publication statusPublished - Jan 2011

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Neutrophil Activation
Respiratory Burst
Periodontitis
Cytokines
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-8
Neutrophils
methionyl-leucyl-phenylalanine
Superoxides
Albumins
Chronic Periodontitis
Antibodies
Luminescence
Neutralizing Antibodies
Serum Albumin
Immunoglobulins
Healthy Volunteers
Enzyme-Linked Immunosorbent Assay
Phenotype

Keywords

  • adult
  • case-control studies
  • chemotactic factors
  • chronic periodontitis
  • cytokines
  • female
  • granulocyte-macrophage colony-stimulating factor
  • humans
  • inflammation mediators
  • interferon-alpha
  • interleukin-8
  • luminescence
  • male
  • middle aged
  • N-formylmethionine leucyl-phenylalanine
  • neutrophil activation
  • plasma
  • recombinant proteins
  • respiratory burst
  • nonparametric statistics
  • chemical stimulation

Cite this

Dias, Irundika H K ; Matthews, John B. ; Chapple, Iain L.C. ; Wright, Helen J. ; Dunston, Christopher R ; Griffiths, Helen R. / Activation of the neutrophil respiratory burst by plasma from periodontitis patients is mediated by pro-inflammatory cytokines. In: Journal of Clinical Periodontology. 2011 ; Vol. 38, No. 1. pp. 1-7.
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abstract = "Aim: To determine the effect of periodontitis patients' plasma on the neutrophil oxidative burst and the role of albumin, immunoglobulins (Igs) and cytokines.Materials and Methods: Plasma was collected from chronic periodontitis patients (n=11) and periodontally healthy controls (n=11) and used with/without depletion of albumin and Ig or antibody neutralization of IL-8, GM-CSF or IFN-a to prime/stimulate peripheral blood neutrophils, isolated from healthy volunteers. The respiratory burst was measured by lucigenin-dependent chemiluminescence. Plasma cytokine levels were determined by ELISA.Results: Plasmas from patients were significantly more effective in both directly stimulating neutrophil superoxide production and priming for subsequent formyl-met-leu-phe (fMLP)-stimulated superoxide production than plasmas from healthy controls (p<0.05). This difference was maintained after depletion of albumin and Ig. Plasma from patients contained higher mean levels of IL-8, GM-CSF and IFN-a. Individual neutralizing antibodies against IL-8, GM-CSF or IFN-a inhibited the direct stimulatory effect of patients' plasma, whereas the ability to prime for fMLP-stimulated superoxide production was only inhibited by neutralization of IFN-a. The stimulating and priming effects of control plasma were unaffected by antibody neutralization.Conclusions: This study demonstrates that plasma cytokines may have a role in inducing the hyperactive (IL-8, GM-CSF, IFN-a) and hyper-reactive (IFN-a) neutrophil phenotype seen in periodontitis patients.",
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Activation of the neutrophil respiratory burst by plasma from periodontitis patients is mediated by pro-inflammatory cytokines. / Dias, Irundika H K; Matthews, John B.; Chapple, Iain L.C.; Wright, Helen J.; Dunston, Christopher R; Griffiths, Helen R.

In: Journal of Clinical Periodontology, Vol. 38, No. 1, 01.2011, p. 1-7.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Activation of the neutrophil respiratory burst by plasma from periodontitis patients is mediated by pro-inflammatory cytokines

AU - Dias, Irundika H K

AU - Matthews, John B.

AU - Chapple, Iain L.C.

AU - Wright, Helen J.

AU - Dunston, Christopher R

AU - Griffiths, Helen R

N1 - © 2010 John Wiley & Sons A/S.

PY - 2011/1

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N2 - Aim: To determine the effect of periodontitis patients' plasma on the neutrophil oxidative burst and the role of albumin, immunoglobulins (Igs) and cytokines.Materials and Methods: Plasma was collected from chronic periodontitis patients (n=11) and periodontally healthy controls (n=11) and used with/without depletion of albumin and Ig or antibody neutralization of IL-8, GM-CSF or IFN-a to prime/stimulate peripheral blood neutrophils, isolated from healthy volunteers. The respiratory burst was measured by lucigenin-dependent chemiluminescence. Plasma cytokine levels were determined by ELISA.Results: Plasmas from patients were significantly more effective in both directly stimulating neutrophil superoxide production and priming for subsequent formyl-met-leu-phe (fMLP)-stimulated superoxide production than plasmas from healthy controls (p<0.05). This difference was maintained after depletion of albumin and Ig. Plasma from patients contained higher mean levels of IL-8, GM-CSF and IFN-a. Individual neutralizing antibodies against IL-8, GM-CSF or IFN-a inhibited the direct stimulatory effect of patients' plasma, whereas the ability to prime for fMLP-stimulated superoxide production was only inhibited by neutralization of IFN-a. The stimulating and priming effects of control plasma were unaffected by antibody neutralization.Conclusions: This study demonstrates that plasma cytokines may have a role in inducing the hyperactive (IL-8, GM-CSF, IFN-a) and hyper-reactive (IFN-a) neutrophil phenotype seen in periodontitis patients.

AB - Aim: To determine the effect of periodontitis patients' plasma on the neutrophil oxidative burst and the role of albumin, immunoglobulins (Igs) and cytokines.Materials and Methods: Plasma was collected from chronic periodontitis patients (n=11) and periodontally healthy controls (n=11) and used with/without depletion of albumin and Ig or antibody neutralization of IL-8, GM-CSF or IFN-a to prime/stimulate peripheral blood neutrophils, isolated from healthy volunteers. The respiratory burst was measured by lucigenin-dependent chemiluminescence. Plasma cytokine levels were determined by ELISA.Results: Plasmas from patients were significantly more effective in both directly stimulating neutrophil superoxide production and priming for subsequent formyl-met-leu-phe (fMLP)-stimulated superoxide production than plasmas from healthy controls (p<0.05). This difference was maintained after depletion of albumin and Ig. Plasma from patients contained higher mean levels of IL-8, GM-CSF and IFN-a. Individual neutralizing antibodies against IL-8, GM-CSF or IFN-a inhibited the direct stimulatory effect of patients' plasma, whereas the ability to prime for fMLP-stimulated superoxide production was only inhibited by neutralization of IFN-a. The stimulating and priming effects of control plasma were unaffected by antibody neutralization.Conclusions: This study demonstrates that plasma cytokines may have a role in inducing the hyperactive (IL-8, GM-CSF, IFN-a) and hyper-reactive (IFN-a) neutrophil phenotype seen in periodontitis patients.

KW - adult

KW - case-control studies

KW - chemotactic factors

KW - chronic periodontitis

KW - cytokines

KW - female

KW - granulocyte-macrophage colony-stimulating factor

KW - humans

KW - inflammation mediators

KW - interferon-alpha

KW - interleukin-8

KW - luminescence

KW - male

KW - middle aged

KW - N-formylmethionine leucyl-phenylalanine

KW - neutrophil activation

KW - plasma

KW - recombinant proteins

KW - respiratory burst

KW - nonparametric statistics

KW - chemical stimulation

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U2 - 10.1111/j.1600-051X.2010.01628.x

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