Activation of the neutrophil respiratory burst by plasma from periodontitis patients is mediated by pro-inflammatory cytokines

Irundika H K Dias, John B. Matthews, Iain L.C. Chapple, Helen J. Wright, Christopher R Dunston, Helen R Griffiths

Research output: Contribution to journalArticle

Abstract

Aim: To determine the effect of periodontitis patients' plasma on the neutrophil oxidative burst and the role of albumin, immunoglobulins (Igs) and cytokines.
Materials and Methods: Plasma was collected from chronic periodontitis patients (n=11) and periodontally healthy controls (n=11) and used with/without depletion of albumin and Ig or antibody neutralization of IL-8, GM-CSF or IFN-a to prime/stimulate peripheral blood neutrophils, isolated from healthy volunteers. The respiratory burst was measured by lucigenin-dependent chemiluminescence. Plasma cytokine levels were determined by ELISA.
Results: Plasmas from patients were significantly more effective in both directly stimulating neutrophil superoxide production and priming for subsequent formyl-met-leu-phe (fMLP)-stimulated superoxide production than plasmas from healthy controls (p<0.05). This difference was maintained after depletion of albumin and Ig. Plasma from patients contained higher mean levels of IL-8, GM-CSF and IFN-a. Individual neutralizing antibodies against IL-8, GM-CSF or IFN-a inhibited the direct stimulatory effect of patients' plasma, whereas the ability to prime for fMLP-stimulated superoxide production was only inhibited by neutralization of IFN-a. The stimulating and priming effects of control plasma were unaffected by antibody neutralization.
Conclusions: This study demonstrates that plasma cytokines may have a role in inducing the hyperactive (IL-8, GM-CSF, IFN-a) and hyper-reactive (IFN-a) neutrophil phenotype seen in periodontitis patients.
Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Clinical Periodontology
Volume38
Issue number1
Early online date21 Oct 2010
DOIs
Publication statusPublished - Jan 2011

Keywords

  • adult
  • case-control studies
  • chemotactic factors
  • chronic periodontitis
  • cytokines
  • female
  • granulocyte-macrophage colony-stimulating factor
  • humans
  • inflammation mediators
  • interferon-alpha
  • interleukin-8
  • luminescence
  • male
  • middle aged
  • N-formylmethionine leucyl-phenylalanine
  • neutrophil activation
  • plasma
  • recombinant proteins
  • respiratory burst
  • nonparametric statistics
  • chemical stimulation

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