Adaptive responses by mouse early embryos to maternal diet protect fetal growth but predispose to adult onset disease

Adam J. Watkins, Elizabeth Ursell, Rose Panton, Tom Papenbrock, Lisa Hollis, Colm Cunningham, Adrian Wilkins, V. Hugh Perry, Bhavwanti Sheth, Wing Yee Wing, Judith J. Eckert, Arthur E. Wild, Mark A. Hanson, Clive Osmond, Tom P. Fleming

Research output: Contribution to journalArticle

Abstract

Poor maternal nutrition during pregnancy can alter postnatal phenotype and increase susceptibility to adult cardiovascular and metabolic diseases. However, underlying mechanisms are largely unknown. Here, we show that maternal low protein diet (LPD), fed exclusively during mouse preimplantation development, leads to offspring with increased weight from birth, sustained hypertension, and abnormal anxiety-related behavior, especially in females. These adverse outcomes were interrelated with increased perinatal weight being predictive of later adult overweight and hypertension. Embryo transfer experiments revealed that the increase in perinatal weight was induced within blastocysts responding to preimplantation LPD, independent of subsequent maternal environment during later pregnancy. We further identified the embryo-derived visceral yolk sac endoderm (VYSE) as one mediator of this response. VYSE contributes to fetal growth through endocytosis of maternal proteins, mainly via the multiligand megalin (LRP2) receptor and supply of liberated amino acids. Thus, LPD maintained throughout gestation stimulated VYSE nutrient transport capacity and megalin expression in late pregnancy, with enhanced megalin expression evident even when LPD was limited to the preimplantation period. Our results demonstrate that in a nutrient-restricted environment, the preimplantation embryo activates physiological mechanisms of developmental plasticity to stablize conceptus growth and enhance postnatal fitness. However, activation of such responses may also lead to adult excess growth and cardiovascular and behavioral diseases.
Original languageEnglish
Pages (from-to)299-306
Number of pages8
JournalBiology of Reproduction
Volume78
Issue number2
Early online date7 Nov 2007
DOIs
Publication statusPublished - 1 Feb 2008

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Protein-Restricted Diet
Low Density Lipoprotein Receptor-Related Protein-2
Fetal Development
Yolk Sac
Endoderm
Embryonic Structures
Mothers
Diet
Blastocyst
Pregnancy
Cardiovascular Diseases
Prenatal Nutritional Physiological Phenomena
Hypertension
Weights and Measures
Food
Embryo Transfer
Metabolic Diseases
Growth
Endocytosis
Birth Weight

Keywords

  • behavior
  • yolk sac
  • preimplantation embryo
  • megalin
  • low protein diet
  • growth
  • environment
  • embryo
  • developmental biology
  • conceptus
  • blood pressure
  • blastocyst

Cite this

Watkins, A. J., Ursell, E., Panton, R., Papenbrock, T., Hollis, L., Cunningham, C., ... Fleming, T. P. (2008). Adaptive responses by mouse early embryos to maternal diet protect fetal growth but predispose to adult onset disease. Biology of Reproduction, 78(2), 299-306. https://doi.org/10.1095/biolreprod.107.064220
Watkins, Adam J. ; Ursell, Elizabeth ; Panton, Rose ; Papenbrock, Tom ; Hollis, Lisa ; Cunningham, Colm ; Wilkins, Adrian ; Perry, V. Hugh ; Sheth, Bhavwanti ; Wing, Wing Yee ; Eckert, Judith J. ; Wild, Arthur E. ; Hanson, Mark A. ; Osmond, Clive ; Fleming, Tom P. / Adaptive responses by mouse early embryos to maternal diet protect fetal growth but predispose to adult onset disease. In: Biology of Reproduction. 2008 ; Vol. 78, No. 2. pp. 299-306.
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Watkins, AJ, Ursell, E, Panton, R, Papenbrock, T, Hollis, L, Cunningham, C, Wilkins, A, Perry, VH, Sheth, B, Wing, WY, Eckert, JJ, Wild, AE, Hanson, MA, Osmond, C & Fleming, TP 2008, 'Adaptive responses by mouse early embryos to maternal diet protect fetal growth but predispose to adult onset disease', Biology of Reproduction, vol. 78, no. 2, pp. 299-306. https://doi.org/10.1095/biolreprod.107.064220

Adaptive responses by mouse early embryos to maternal diet protect fetal growth but predispose to adult onset disease. / Watkins, Adam J.; Ursell, Elizabeth; Panton, Rose; Papenbrock, Tom; Hollis, Lisa; Cunningham, Colm; Wilkins, Adrian; Perry, V. Hugh; Sheth, Bhavwanti; Wing, Wing Yee; Eckert, Judith J.; Wild, Arthur E.; Hanson, Mark A.; Osmond, Clive; Fleming, Tom P.

In: Biology of Reproduction, Vol. 78, No. 2, 01.02.2008, p. 299-306.

Research output: Contribution to journalArticle

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AU - Panton, Rose

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AU - Hollis, Lisa

AU - Cunningham, Colm

AU - Wilkins, Adrian

AU - Perry, V. Hugh

AU - Sheth, Bhavwanti

AU - Wing, Wing Yee

AU - Eckert, Judith J.

AU - Wild, Arthur E.

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AU - Osmond, Clive

AU - Fleming, Tom P.

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