The natural selection of anticoagulant resistant rats has resulted in a need for an alternative to anticoagulant rodenticides which differs in both active ingredient and in the method of dosing. Cholecalciferol toxicity to rodents using the dermal route is demonstrated using a variety of penetration enhancing formulations in two in-vitro models and finally in-vivo. A 1 ml dose of 50/50 (v/v) DMSO/ethanol containing 15% (v/v) PEG 200 and 20% (w/v) cholecalciferol was judged as 'sufficiently effective' in line with the European Union's Biocidal Products Regulation (No. 528/2012) during in-vivo studies. This dose was found to cause 100% mortality in a rat population in 64.4 h (±22 h).
|Number of pages||9|
|Journal||International Journal of Pharmaceutics|
|Early online date||30 Mar 2015|
|Publication status||Published - 20 Jun 2015|
Bibliographical noteAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0).
Funding: European Union’s Seventh Framework Programme managed by REA Research Executive Agency http://ec.europa.eu/rea (FP7/2007-2013) under grant Project Reference 286852.
- regulation No. 528/2012