Attenuation of muscle wasting in murine C2C12myotubes by epigallocatechin-3-gallate

Kamran A. Mirza, Suzette L. Pereira, Neile K. Edens, Michael J. Tisdale

Research output: Contribution to journalArticle

Abstract

Background: Loss of muscle protein is a common feature of wasting diseases where currently treatment is limited. This study investigates the potential of epigallocatechin-3-gallate (EGCg), the most abundant catechin in green tea, to reverse the increased protein degradation and rescue the decreased protein synthesis which leads to muscle atrophy.

Methods: Studies were conducted in vitro using murine C2C12myotubes. Increased protein degradation and reduced rates of protein synthesis were induced by serum starvation and tumour necrosis factor-α (TNF-α).

Results: EGCg effectively attenuated the depression of protein synthesis and increase in protein degradation in murine myotubes at concentrations as low as 10 μM. Serum starvation increased expression of the proteasome 20S and 19S subunits, as well as the proteasome ‘chymotrypsin-like’ enzyme activity, and these were all attenuated down to basal values in the presence of EGCg. Serum starvation did not increase expression of the ubiquitin ligases MuRF1 and MAFbx, but EGCg reduced their expression below basal levels, possibly due to an increased expression of phospho Akt (pAkt) and phospho forkhead box O3a (pFoxO3a). Attenuation of protein degradation by EGCg was increased in the presence of ZnSO4, suggesting an EGCg-Zn2+complex may be the active species.

Conclusion: The ability of EGCg to attenuate depressed protein synthesis and increase protein degradation in the myotubule model system suggests that it may be effective in preserving skeletal muscle mass in catabolic conditions.

LanguageEnglish
Pages339-345
Number of pages7
JournalJournal of Cachexia, Sarcopenia and Muscle
Volume5
Issue number4
Early online date20 Mar 2014
DOIs
Publication statusPublished - Dec 2014

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Proteolysis
Muscles
Starvation
Proteasome Endopeptidase Complex
Proteins
Serum
Wasting Syndrome
Muscular Atrophy
Catechin
Muscle Proteins
Skeletal Muscle Fibers
Tea
Ligases
Ubiquitin
epigallocatechin gallate
Skeletal Muscle
Tumor Necrosis Factor-alpha

Bibliographical note

© 2014 The Authors. Published by John Wiley & Sons Ltd on behalf of the Society on Sarcopenia, Cachexia and Wasting Disorders
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

Keywords

  • epigallocatechin gallate
  • proteasome
  • protein degradation
  • protein synthesis
  • tumour necrosis factor-α
  • zinc

Cite this

Mirza, Kamran A. ; Pereira, Suzette L. ; Edens, Neile K. ; Tisdale, Michael J. / Attenuation of muscle wasting in murine C2C12myotubes by epigallocatechin-3-gallate. In: Journal of Cachexia, Sarcopenia and Muscle. 2014 ; Vol. 5, No. 4. pp. 339-345.
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Attenuation of muscle wasting in murine C2C12myotubes by epigallocatechin-3-gallate. / Mirza, Kamran A.; Pereira, Suzette L.; Edens, Neile K.; Tisdale, Michael J.

In: Journal of Cachexia, Sarcopenia and Muscle, Vol. 5, No. 4, 12.2014, p. 339-345.

Research output: Contribution to journalArticle

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AU - Mirza, Kamran A.

AU - Pereira, Suzette L.

AU - Edens, Neile K.

AU - Tisdale, Michael J.

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