Characterisation of Aeromonas strains and species by pulsed field gel electrophoresis and principal components analysis

M.A. Livesley, S.N. Smith, Richard A. Armstrong, G.A. Barker

Research output: Contribution to journalArticle

Abstract

Aeromonas genomes were investigated by restriction digesting chromosomal DNA with the endonuclease XbaI, separation of restriction fragments by pulsed field gel electrophoresis (PFGE) and principal components analysis (PCA) of resulting separation patterns. A. salmonicida salmonicida were unique amongst the isolates investigated. Separation profiles of these isolates were similar and all characterised by a distinct absence of bands in the 250kb region. Principal components analysis represented these strains as a clearly defined homogeneous group separated by insignificant Euclidian distances. However, A. salmonicida achromogenes isolates in common with those of A. hydrophila and A. sobria were shown by principal components analysis to be more heterogeneous in nature. Fragments from these isolates were more uniform in size distribution but as demonstrated by the Euclidian distances attained through PCA potentially characteristic of each strain. Furthermore passaging of Aeromonas isolates through an appropriate host did not greatly modify fragment separation profiles, indicative of the genomic stability of test aeromonads and the potential of restriction digesting/PFGE/PCA in Aeromonas typing.

Original languageEnglish
Pages (from-to)369-375
Number of pages7
JournalJournal of Fish Diseases
Volume22
Issue number5
DOIs
Publication statusPublished - Sep 1999

Fingerprint

Aeromonas
Pulsed Field Gel Electrophoresis
pulsed-field gel electrophoresis
Principal Component Analysis
electrokinesis
principal component analysis
gel
Genomic Instability
Deoxyribonuclease I
genomics
genome
Genome
DNA
testing

Keywords

  • aeromonas genomes
  • restriction digesting chromosomal DNA
  • endonuclease XbaI
  • aeromonas salmonicida salmonicida

Cite this

Livesley, M.A. ; Smith, S.N. ; Armstrong, Richard A. ; Barker, G.A. / Characterisation of Aeromonas strains and species by pulsed field gel electrophoresis and principal components analysis. In: Journal of Fish Diseases. 1999 ; Vol. 22, No. 5. pp. 369-375.
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Characterisation of Aeromonas strains and species by pulsed field gel electrophoresis and principal components analysis. / Livesley, M.A.; Smith, S.N.; Armstrong, Richard A.; Barker, G.A.

In: Journal of Fish Diseases, Vol. 22, No. 5, 09.1999, p. 369-375.

Research output: Contribution to journalArticle

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