HPLC MS/MS has shown great potential in the measurement of DNA oxidative damage. Its accuracy depends on the use of multiply isotopically labelled internal standards. In this report, multiply isotopically labelled (M + 4) guanine internal standards were prepared in the form of base, nucleoside, as well as DNA oligomer. To our knowledge, this is the first chemical synthesis of oligomers containing (M + 4) guanine, and we believe that they can be used to develop a procedure that can make further improvement to the existing analytical procedures. Copyright © Taylor & Francis, Inc.
Bibliographical note16th International Roundtable of the International Society for Nucleosides Nucleotides and Nucleic Acids (IS3NA), Minneapolis, MN (US), 12-16 September 2004
- DNA oxidative damage
- internal standard
- isotopically labelled guanine