Comparison of nonlinear properties of monomer and dimer of bacterial phytochrome from Deinococcus radiodurans

Diana I. Galiakhmetova; Aleksandr S. Koviarov; Viktor V. Dremin; Andrei A. Gorodetsky; Marios Maimaris; Dmitrii A. Stoliarov; Mikhail Baloban; Vladislav V. Verkhusha; Sergei G. Sokolovski; Edik U. Rafailov

doi:10.1117/12.2666486

Comparison of nonlinear properties of monomer and dimer of bacterial phytochrome from Deinococcus radiodurans

Diana I. Galiakhmetova^*, Aleksandr S. Koviarov, Viktor V. Dremin, Andrei A. Gorodetsky, Marios Maimaris, Dmitrii A. Stoliarov, Mikhail Baloban, Vladislav V. Verkhusha, Sergei G. Sokolovski, Edik U. Rafailov

^*Corresponding author for this work

Research output: Contribution to journal › Conference article › peer-review

Abstract

Current medicine might be greatly enhanced by the ability to in vivo control and monitor neurons using opsins/phytochromes expressed in neural cells. The fundamental challenge with non-invasive neural cell activity regulation is a high absorption of visible light into biological tissues. This drawback could be mitigated by the photoconversion of phytochromes in spectral ranges with higher tissue transparency. In this study, we first demonstrated two-photon Pr-Pfr conversion of monomeric phytochrome at 1.2 µm wavelength. We did a comparison of linear and nonlinear conversion of truncated DrBphP bacterial phytochromes. This work provides a structured understanding of the optical properties of the dimer and monomer of phytochrome as well as their potential for use in optogenetics.

Original language	English
Article number	126272T
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	12627
DOIs	https://doi.org/10.1117/12.2666486
Publication status	Published - 11 Aug 2023
Event	European Conferences on Biomedical Optics 2023: Translational Biophotonics: Diagnostics and Therapeutics - Munich, Germany Duration: 25 Jun 2023 → 29 Jun 2023

Bibliographical note

Funding Information:
This work has been supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No.863214 – NEUROPA project. D.I.G. acknowledges the Rank Prize for the Return to Research grant.

Copyright 2023 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Keywords

Adimer
iRFP
Monomer
Nonlinear conversion
Optogenetics
Pfr state
Phytochrome
Pr state
Tunable ultra-short pulsed laser
Two-photon photoconversion

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10.1117/12.2666486

126272T
Copyright 2023 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
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Galiakhmetova, D. I., Koviarov, A. S., Dremin, V. V., Gorodetsky, A. A., Maimaris, M., Stoliarov, D. A., Baloban, M., Verkhusha, V. V., Sokolovski, S. G., & Rafailov, E. U. (2023). Comparison of nonlinear properties of monomer and dimer of bacterial phytochrome from Deinococcus radiodurans. Proceedings of SPIE - The International Society for Optical Engineering, 12627, Article 126272T. https://doi.org/10.1117/12.2666486

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title = "Comparison of nonlinear properties of monomer and dimer of bacterial phytochrome from Deinococcus radiodurans",

abstract = "Current medicine might be greatly enhanced by the ability to in vivo control and monitor neurons using opsins/phytochromes expressed in neural cells. The fundamental challenge with non-invasive neural cell activity regulation is a high absorption of visible light into biological tissues. This drawback could be mitigated by the photoconversion of phytochromes in spectral ranges with higher tissue transparency. In this study, we first demonstrated two-photon Pr-Pfr conversion of monomeric phytochrome at 1.2 µm wavelength. We did a comparison of linear and nonlinear conversion of truncated DrBphP bacterial phytochromes. This work provides a structured understanding of the optical properties of the dimer and monomer of phytochrome as well as their potential for use in optogenetics.",

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author = "Galiakhmetova, {Diana I.} and Koviarov, {Aleksandr S.} and Dremin, {Viktor V.} and Gorodetsky, {Andrei A.} and Marios Maimaris and Stoliarov, {Dmitrii A.} and Mikhail Baloban and Verkhusha, {Vladislav V.} and Sokolovski, {Sergei G.} and Rafailov, {Edik U.}",

note = "Funding Information: This work has been supported by the European Union{\textquoteright}s Horizon 2020 research and innovation programme under grant agreement No.863214 – NEUROPA project. D.I.G. acknowledges the Rank Prize for the Return to Research grant. Copyright 2023 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. ; European Conferences on Biomedical Optics 2023 : Translational Biophotonics: Diagnostics and Therapeutics ; Conference date: 25-06-2023 Through 29-06-2023",

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month = aug,

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Galiakhmetova, DI, Koviarov, AS, Dremin, VV, Gorodetsky, AA, Maimaris, M, Stoliarov, DA, Baloban, M, Verkhusha, VV, Sokolovski, SG & Rafailov, EU 2023, 'Comparison of nonlinear properties of monomer and dimer of bacterial phytochrome from Deinococcus radiodurans', Proceedings of SPIE - The International Society for Optical Engineering, vol. 12627, 126272T. https://doi.org/10.1117/12.2666486

Comparison of nonlinear properties of monomer and dimer of bacterial phytochrome from Deinococcus radiodurans. / Galiakhmetova, Diana I.; Koviarov, Aleksandr S.; Dremin, Viktor V. et al.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 12627, 126272T, 11.08.2023.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - Comparison of nonlinear properties of monomer and dimer of bacterial phytochrome from Deinococcus radiodurans

AU - Galiakhmetova, Diana I.

AU - Koviarov, Aleksandr S.

AU - Dremin, Viktor V.

AU - Gorodetsky, Andrei A.

AU - Maimaris, Marios

AU - Stoliarov, Dmitrii A.

AU - Baloban, Mikhail

AU - Verkhusha, Vladislav V.

AU - Sokolovski, Sergei G.

AU - Rafailov, Edik U.

N1 - Funding Information: This work has been supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No.863214 – NEUROPA project. D.I.G. acknowledges the Rank Prize for the Return to Research grant. Copyright 2023 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

PY - 2023/8/11

Y1 - 2023/8/11

N2 - Current medicine might be greatly enhanced by the ability to in vivo control and monitor neurons using opsins/phytochromes expressed in neural cells. The fundamental challenge with non-invasive neural cell activity regulation is a high absorption of visible light into biological tissues. This drawback could be mitigated by the photoconversion of phytochromes in spectral ranges with higher tissue transparency. In this study, we first demonstrated two-photon Pr-Pfr conversion of monomeric phytochrome at 1.2 µm wavelength. We did a comparison of linear and nonlinear conversion of truncated DrBphP bacterial phytochromes. This work provides a structured understanding of the optical properties of the dimer and monomer of phytochrome as well as their potential for use in optogenetics.

AB - Current medicine might be greatly enhanced by the ability to in vivo control and monitor neurons using opsins/phytochromes expressed in neural cells. The fundamental challenge with non-invasive neural cell activity regulation is a high absorption of visible light into biological tissues. This drawback could be mitigated by the photoconversion of phytochromes in spectral ranges with higher tissue transparency. In this study, we first demonstrated two-photon Pr-Pfr conversion of monomeric phytochrome at 1.2 µm wavelength. We did a comparison of linear and nonlinear conversion of truncated DrBphP bacterial phytochromes. This work provides a structured understanding of the optical properties of the dimer and monomer of phytochrome as well as their potential for use in optogenetics.

KW - Adimer

KW - iRFP

KW - Monomer

KW - Nonlinear conversion

KW - Optogenetics

KW - Pfr state

KW - Phytochrome

KW - Pr state

KW - Tunable ultra-short pulsed laser

KW - Two-photon photoconversion

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U2 - 10.1117/12.2666486

DO - 10.1117/12.2666486

M3 - Conference article

AN - SCOPUS:85172206947

SN - 0277-786X

VL - 12627

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

M1 - 126272T

T2 - European Conferences on Biomedical Optics 2023

Y2 - 25 June 2023 through 29 June 2023

ER -

Comparison of nonlinear properties of monomer and dimer of bacterial phytochrome from Deinococcus radiodurans

Abstract

Bibliographical note

Keywords

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