Comparison of nonlinear properties of monomer and dimer of bacterial phytochrome from Deinococcus radiodurans

Diana I. Galiakhmetova*, Aleksandr S. Koviarov, Viktor V. Dremin, Andrei A. Gorodetsky, Marios Maimaris, Dmitrii A. Stoliarov, Mikhail Baloban, Vladislav V. Verkhusha, Sergei G. Sokolovski, Edik U. Rafailov

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review


Current medicine might be greatly enhanced by the ability to in vivo control and monitor neurons using opsins/phytochromes expressed in neural cells. The fundamental challenge with non-invasive neural cell activity regulation is a high absorption of visible light into biological tissues. This drawback could be mitigated by the photoconversion of phytochromes in spectral ranges with higher tissue transparency. In this study, we first demonstrated two-photon Pr-Pfr conversion of monomeric phytochrome at 1.2 µm wavelength. We did a comparison of linear and nonlinear conversion of truncated DrBphP bacterial phytochromes. This work provides a structured understanding of the optical properties of the dimer and monomer of phytochrome as well as their potential for use in optogenetics.

Original languageEnglish
Article number126272T
JournalProceedings of SPIE - The International Society for Optical Engineering
Publication statusPublished - 11 Aug 2023
EventEuropean Conferences on Biomedical Optics 2023: Translational Biophotonics: Diagnostics and Therapeutics - Munich, Germany
Duration: 25 Jun 202329 Jun 2023

Bibliographical note

Funding Information:
This work has been supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No.863214 – NEUROPA project. D.I.G. acknowledges the Rank Prize for the Return to Research grant.

Copyright 2023 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.


  • Adimer
  • iRFP
  • Monomer
  • Nonlinear conversion
  • Optogenetics
  • Pfr state
  • Phytochrome
  • Pr state
  • Tunable ultra-short pulsed laser
  • Two-photon photoconversion


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