Development of a quality control material for the measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an in vivo marker of oxidative stress, and comparison of results from different laboratories

J Lunec; Karl E. Herbert; George D.D. Jones; L. Dickinson; M. Evans; N. Mistry; D. Chauhan; G. Capper; Qinguo Zheng

Development of a quality control material for the measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an in vivo marker of oxidative stress, and comparison of results from different laboratories

J Lunec, Karl E. Herbert, George D.D. Jones, L. Dickinson, M. Evans, N. Mistry, D. Chauhan, G. Capper, Qinguo Zheng

Research output: Contribution to journal › Article › peer-review

Abstract

The measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine is an increasingly popular marker of in vivo oxidative damage to DNA. A random-sequence 21-mer oligonucleotide 5'-TCA GXC GTA CGT GAT CTC AGT-3' in which X was 8-oxo-guanine (8-oxo-G) was purified and accurate determination of the oxidised base was confirmed by a 32P-end labelling strategy. The lyophilised material was analysed for its absolute content of 8-oxo-dG by several major laboratories in Europe and one in Japan. Most laboratories using HPLC-ECD underestimated, while GC-MS-SIM overestimated the level of the lesion. HPLC-ECD measured the target value with greatest accuracy. The results also suggest that none of the procedures can accurately quantitate levels of 1 in 10(6) 8-oxo-(d)G in DNA.

Original language	English
Pages (from-to)	S27-S31
Journal	Free Radical Research
Volume	33
Issue number	Suppl. S
Publication status	Published - Nov 2000

Keywords

8-oxo-dG
quality control
accuracy

Cite this

@article{603f57fd95ad4313b074033207a333ab,

title = "Development of a quality control material for the measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an in vivo marker of oxidative stress, and comparison of results from different laboratories",

abstract = "The measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine is an increasingly popular marker of in vivo oxidative damage to DNA. A random-sequence 21-mer oligonucleotide 5'-TCA GXC GTA CGT GAT CTC AGT-3' in which X was 8-oxo-guanine (8-oxo-G) was purified and accurate determination of the oxidised base was confirmed by a 32P-end labelling strategy. The lyophilised material was analysed for its absolute content of 8-oxo-dG by several major laboratories in Europe and one in Japan. Most laboratories using HPLC-ECD underestimated, while GC-MS-SIM overestimated the level of the lesion. HPLC-ECD measured the target value with greatest accuracy. The results also suggest that none of the procedures can accurately quantitate levels of 1 in 10(6) 8-oxo-(d)G in DNA.",

keywords = "8-oxo-dG, quality control, accuracy",

author = "J Lunec and Herbert, {Karl E.} and Jones, {George D.D.} and L. Dickinson and M. Evans and N. Mistry and D. Chauhan and G. Capper and Qinguo Zheng",

year = "2000",

month = nov,

language = "English",

volume = "33",

pages = "S27--S31",

journal = "Free Radical Research",

issn = "1071-5762",

publisher = "Informa Healthcare",

number = "Suppl. S",

}

Development of a quality control material for the measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an in vivo marker of oxidative stress, and comparison of results from different laboratories. / Lunec, J; Herbert, Karl E.; Jones, George D.D. et al.
In: Free Radical Research, Vol. 33, No. Suppl. S, 11.2000, p. S27-S31.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Development of a quality control material for the measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an in vivo marker of oxidative stress, and comparison of results from different laboratories

AU - Lunec, J

AU - Herbert, Karl E.

AU - Jones, George D.D.

AU - Dickinson, L.

AU - Evans, M.

AU - Mistry, N.

AU - Chauhan, D.

AU - Capper, G.

AU - Zheng, Qinguo

PY - 2000/11

Y1 - 2000/11

N2 - The measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine is an increasingly popular marker of in vivo oxidative damage to DNA. A random-sequence 21-mer oligonucleotide 5'-TCA GXC GTA CGT GAT CTC AGT-3' in which X was 8-oxo-guanine (8-oxo-G) was purified and accurate determination of the oxidised base was confirmed by a 32P-end labelling strategy. The lyophilised material was analysed for its absolute content of 8-oxo-dG by several major laboratories in Europe and one in Japan. Most laboratories using HPLC-ECD underestimated, while GC-MS-SIM overestimated the level of the lesion. HPLC-ECD measured the target value with greatest accuracy. The results also suggest that none of the procedures can accurately quantitate levels of 1 in 10(6) 8-oxo-(d)G in DNA.

AB - The measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine is an increasingly popular marker of in vivo oxidative damage to DNA. A random-sequence 21-mer oligonucleotide 5'-TCA GXC GTA CGT GAT CTC AGT-3' in which X was 8-oxo-guanine (8-oxo-G) was purified and accurate determination of the oxidised base was confirmed by a 32P-end labelling strategy. The lyophilised material was analysed for its absolute content of 8-oxo-dG by several major laboratories in Europe and one in Japan. Most laboratories using HPLC-ECD underestimated, while GC-MS-SIM overestimated the level of the lesion. HPLC-ECD measured the target value with greatest accuracy. The results also suggest that none of the procedures can accurately quantitate levels of 1 in 10(6) 8-oxo-(d)G in DNA.

KW - 8-oxo-dG

KW - quality control

KW - accuracy

UR - http://informahealthcare.com/journal/fra

M3 - Article

SN - 1071-5762

VL - 33

SP - S27-S31

JO - Free Radical Research

JF - Free Radical Research

IS - Suppl. S

ER -

Development of a quality control material for the measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an in vivo marker of oxidative stress, and comparison of results from different laboratories

Abstract

Keywords

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