Development of a quality control material for the measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an in vivo marker of oxidative stress, and comparison of results from different laboratories

J Lunec, Karl E. Herbert, George D.D. Jones, L. Dickinson, M. Evans, N. Mistry, D. Chauhan, G. Capper, Qinguo Zheng

Research output: Contribution to journalArticle

Abstract

The measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine is an increasingly popular marker of in vivo oxidative damage to DNA. A random-sequence 21-mer oligonucleotide 5'-TCA GXC GTA CGT GAT CTC AGT-3' in which X was 8-oxo-guanine (8-oxo-G) was purified and accurate determination of the oxidised base was confirmed by a 32P-end labelling strategy. The lyophilised material was analysed for its absolute content of 8-oxo-dG by several major laboratories in Europe and one in Japan. Most laboratories using HPLC-ECD underestimated, while GC-MS-SIM overestimated the level of the lesion. HPLC-ECD measured the target value with greatest accuracy. The results also suggest that none of the procedures can accurately quantitate levels of 1 in 10(6) 8-oxo-(d)G in DNA.
Original languageEnglish
Pages (from-to)S27-S31
JournalFree Radical Research
Volume33
Issue numberSuppl. S
Publication statusPublished - Nov 2000

Fingerprint

Oxidative stress
Quality Control
Quality control
Oxidative Stress
High Pressure Liquid Chromatography
DNA
Guanine
Oligonucleotides
Labeling
DNA Damage
Japan
8-oxo-7-hydrodeoxyguanosine
GAT

Keywords

  • 8-oxo-dG
  • quality control
  • accuracy

Cite this

Lunec, J ; Herbert, Karl E. ; Jones, George D.D. ; Dickinson, L. ; Evans, M. ; Mistry, N. ; Chauhan, D. ; Capper, G. ; Zheng, Qinguo. / Development of a quality control material for the measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an in vivo marker of oxidative stress, and comparison of results from different laboratories. In: Free Radical Research. 2000 ; Vol. 33, No. Suppl. S. pp. S27-S31.
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abstract = "The measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine is an increasingly popular marker of in vivo oxidative damage to DNA. A random-sequence 21-mer oligonucleotide 5'-TCA GXC GTA CGT GAT CTC AGT-3' in which X was 8-oxo-guanine (8-oxo-G) was purified and accurate determination of the oxidised base was confirmed by a 32P-end labelling strategy. The lyophilised material was analysed for its absolute content of 8-oxo-dG by several major laboratories in Europe and one in Japan. Most laboratories using HPLC-ECD underestimated, while GC-MS-SIM overestimated the level of the lesion. HPLC-ECD measured the target value with greatest accuracy. The results also suggest that none of the procedures can accurately quantitate levels of 1 in 10(6) 8-oxo-(d)G in DNA.",
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Lunec, J, Herbert, KE, Jones, GDD, Dickinson, L, Evans, M, Mistry, N, Chauhan, D, Capper, G & Zheng, Q 2000, 'Development of a quality control material for the measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an in vivo marker of oxidative stress, and comparison of results from different laboratories', Free Radical Research, vol. 33, no. Suppl. S, pp. S27-S31.

Development of a quality control material for the measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an in vivo marker of oxidative stress, and comparison of results from different laboratories. / Lunec, J; Herbert, Karl E.; Jones, George D.D.; Dickinson, L.; Evans, M.; Mistry, N.; Chauhan, D.; Capper, G.; Zheng, Qinguo.

In: Free Radical Research, Vol. 33, No. Suppl. S, 11.2000, p. S27-S31.

Research output: Contribution to journalArticle

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AU - Lunec, J

AU - Herbert, Karl E.

AU - Jones, George D.D.

AU - Dickinson, L.

AU - Evans, M.

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AU - Capper, G.

AU - Zheng, Qinguo

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AB - The measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine is an increasingly popular marker of in vivo oxidative damage to DNA. A random-sequence 21-mer oligonucleotide 5'-TCA GXC GTA CGT GAT CTC AGT-3' in which X was 8-oxo-guanine (8-oxo-G) was purified and accurate determination of the oxidised base was confirmed by a 32P-end labelling strategy. The lyophilised material was analysed for its absolute content of 8-oxo-dG by several major laboratories in Europe and one in Japan. Most laboratories using HPLC-ECD underestimated, while GC-MS-SIM overestimated the level of the lesion. HPLC-ECD measured the target value with greatest accuracy. The results also suggest that none of the procedures can accurately quantitate levels of 1 in 10(6) 8-oxo-(d)G in DNA.

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