Development of a quality control material for the measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an in vivo marker of oxidative stress, and comparison of results from different laboratories

J Lunec, Karl E. Herbert, George D.D. Jones, L. Dickinson, M. Evans, N. Mistry, D. Chauhan, G. Capper, Qinguo Zheng

Research output: Contribution to journalArticle

Abstract

The measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine is an increasingly popular marker of in vivo oxidative damage to DNA. A random-sequence 21-mer oligonucleotide 5'-TCA GXC GTA CGT GAT CTC AGT-3' in which X was 8-oxo-guanine (8-oxo-G) was purified and accurate determination of the oxidised base was confirmed by a 32P-end labelling strategy. The lyophilised material was analysed for its absolute content of 8-oxo-dG by several major laboratories in Europe and one in Japan. Most laboratories using HPLC-ECD underestimated, while GC-MS-SIM overestimated the level of the lesion. HPLC-ECD measured the target value with greatest accuracy. The results also suggest that none of the procedures can accurately quantitate levels of 1 in 10(6) 8-oxo-(d)G in DNA.
Original languageEnglish
Pages (from-to)S27-S31
JournalFree Radical Research
Volume33
Issue numberSuppl. S
Publication statusPublished - Nov 2000

Keywords

  • 8-oxo-dG
  • quality control
  • accuracy

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    Lunec, J., Herbert, K. E., Jones, G. D. D., Dickinson, L., Evans, M., Mistry, N., Chauhan, D., Capper, G., & Zheng, Q. (2000). Development of a quality control material for the measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an in vivo marker of oxidative stress, and comparison of results from different laboratories. Free Radical Research, 33(Suppl. S), S27-S31.