Dopaminergic suppression of synaptic transmission in the lateral entorhinal cortex

Douglas A. Caruana, C. Andrew Chapman*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Dopaminergic projections to the superficial layers of the lateral entorhinal cortex can modulate the strength of olfactory inputs to the region. We have found that low concentrations of dopamine facilitate field EPSPs in the entorhinal cortex, and that higher concentrations of dopamine suppress synaptic responses. Here, we have used whole-cell current clamp recordings from layer II neurons to determine the mechanisms of the suppression. Dopamine (10 to 50 μM) hyperpolarized membrane potential and reversibly suppressed the amplitude of EPSPs evoked by layer I stimulation. Both AMPA- and NMDA-mediated components were suppressed, and paired-pulse facilitation was also enhanced indicating that the suppression is mediated largely by reduced glutamate release. Blockade of D2-like receptors greatly reduced the suppression of EPSPs. Dopamine also lowered input resistance, and reduced the number of action potentials evoked by depolarizing current steps. The drop in input resistance was mediated by activation of D1-like receptors, and was prevented by blocking K+ channels with TEA. The dopaminergic suppression of synaptic transmission is therefore mediated by a D2 receptor-dependent reduction in transmitter release, and a D1 receptor-dependent increase in a K+ conductance. This suppression of EPSPs may dampen the strength of sensory inputs during periods of elevated mesocortical dopamine activity.

Original languageEnglish
Article number203514
JournalNeural Plasticity
Volume2008
DOIs
Publication statusPublished - 1 Jun 2008

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Entorhinal Cortex
Synaptic Transmission
Excitatory Postsynaptic Potentials
Dopamine
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
N-Methylaspartate
Membrane Potentials
Action Potentials
Glutamic Acid
Neurons

Bibliographical note

© 2008 Douglas A. Caruana and C. Andrew Chapman. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this

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Dopaminergic suppression of synaptic transmission in the lateral entorhinal cortex. / Caruana, Douglas A.; Chapman, C. Andrew.

In: Neural Plasticity, Vol. 2008, 203514, 01.06.2008.

Research output: Contribution to journalArticle

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