Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells

Anna Khokhlova; Igor Zolotovskii; Evgeniia Pogodina; Yuri Saenko; Dmitrii Stoliarov; Svetlana Vorsina; Andrei Fotiadi; Daria Liamina; Sergei Sokolovski; Edik Rafailov; Michael R. Hamblin; James D. Carroll; Praveen Arany

doi:10.1117/12.2509529

Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells

Anna Khokhlova, Igor Zolotovskii, Evgeniia Pogodina, Yuri Saenko, Dmitrii Stoliarov, Svetlana Vorsina, Andrei Fotiadi, Daria Liamina, Sergei Sokolovski, Edik Rafailov, Michael R. Hamblin (Editor), James D. Carroll (Editor), Praveen Arany (Editor)

Research output: Contribution to journal › Conference article › peer-review

Abstract

The mechanism responsible for the oxidative stress due to photobiomodulation induced by 1265 nm laser is still unclear. Mitochondria are assumed to be the most probable acceptors of the 1265 nm laser irradiation. We study oxidative stress, mitochondrial potential, GSH, cell viability, DNA damage. We demonstrated that narrowband (highcoherent) and wideband lasers employed at the doses of 9.45 and 66.6-400 J/cm2, respectively, induce a dose-dependent cell death, increase ROS level, disturb mitochondrial functioning and can damage DNA. Thus, the 1265 nm lasers can affect the HCT116 cells through mitochondrial damage. Energy density increase contributes to cell damaging without heating effects.

Original language	English
Article number	108610L
Journal	Proceedings of SPIE - International Society for Optical Engineering
Volume	10861
DOIs	https://doi.org/10.1117/12.2509529
Publication status	Published - 7 Mar 2019
Event	Mechanisms of Photobiomodulation Therapy XIV - San Francisco, United States Duration: 2 Feb 2019 → 7 Feb 2019

Bibliographical note

Copyright 2019 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Keywords

1265 nm laser irradiation
DNA damage
cancer cells
near-infrared lasers
oxidative stress
photobiomodulation therapy
reactive oxygen species
singlet oxygen

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10.1117/12.2509529

Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells
Copyright 2019 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
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Khokhlova, A., Zolotovskii, I., Pogodina, E., Saenko, Y., Stoliarov, D., Vorsina, S., Fotiadi, A., Liamina, D., Sokolovski, S., Rafailov, E., Hamblin, M. R. (Ed.), Carroll, J. D. (Ed.), & Arany, P. (Ed.) (2019). Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells. Proceedings of SPIE - International Society for Optical Engineering, 10861, [108610L ]. https://doi.org/10.1117/12.2509529

Khokhlova, Anna ; Zolotovskii, Igor ; Pogodina, Evgeniia ; Saenko, Yuri ; Stoliarov, Dmitrii ; Vorsina, Svetlana ; Fotiadi, Andrei ; Liamina, Daria ; Sokolovski, Sergei ; Rafailov, Edik ; Hamblin, Michael R. (Editor) ; Carroll, James D. (Editor) ; Arany, Praveen (Editor). / Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells. In: Proceedings of SPIE - International Society for Optical Engineering. 2019 ; Vol. 10861.

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title = "Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells",

abstract = "The mechanism responsible for the oxidative stress due to photobiomodulation induced by 1265 nm laser is still unclear. Mitochondria are assumed to be the most probable acceptors of the 1265 nm laser irradiation. We study oxidative stress, mitochondrial potential, GSH, cell viability, DNA damage. We demonstrated that narrowband (highcoherent) and wideband lasers employed at the doses of 9.45 and 66.6-400 J/cm2, respectively, induce a dose-dependent cell death, increase ROS level, disturb mitochondrial functioning and can damage DNA. Thus, the 1265 nm lasers can affect the HCT116 cells through mitochondrial damage. Energy density increase contributes to cell damaging without heating effects.",

keywords = "1265 nm laser irradiation, DNA damage, cancer cells, near-infrared lasers, oxidative stress, photobiomodulation therapy, reactive oxygen species, singlet oxygen",

author = "Anna Khokhlova and Igor Zolotovskii and Evgeniia Pogodina and Yuri Saenko and Dmitrii Stoliarov and Svetlana Vorsina and Andrei Fotiadi and Daria Liamina and Sergei Sokolovski and Edik Rafailov and Hamblin, {Michael R.} and Carroll, {James D.} and Praveen Arany",

note = "Copyright 2019 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. ; Mechanisms of Photobiomodulation Therapy XIV ; Conference date: 02-02-2019 Through 07-02-2019",

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Khokhlova, A, Zolotovskii, I, Pogodina, E, Saenko, Y, Stoliarov, D, Vorsina, S, Fotiadi, A, Liamina, D, Sokolovski, S , Rafailov, E, Hamblin, MR (ed.), Carroll, JD (ed.) & Arany, P (ed.) 2019, 'Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells', Proceedings of SPIE - International Society for Optical Engineering, vol. 10861, 108610L . https://doi.org/10.1117/12.2509529

Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells. / Khokhlova, Anna; Zolotovskii, Igor; Pogodina, Evgeniia; Saenko, Yuri; Stoliarov, Dmitrii; Vorsina, Svetlana; Fotiadi, Andrei; Liamina, Daria; Sokolovski, Sergei ; Rafailov, Edik; Hamblin, Michael R. (Editor); Carroll, James D. (Editor); Arany, Praveen (Editor).

In: Proceedings of SPIE - International Society for Optical Engineering, Vol. 10861, 108610L , 07.03.2019.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells

AU - Khokhlova, Anna

AU - Zolotovskii, Igor

AU - Pogodina, Evgeniia

AU - Saenko, Yuri

AU - Stoliarov, Dmitrii

AU - Vorsina, Svetlana

AU - Fotiadi, Andrei

AU - Liamina, Daria

AU - Sokolovski, Sergei

AU - Rafailov, Edik

A2 - Hamblin, Michael R.

A2 - Carroll, James D.

A2 - Arany, Praveen

N1 - Copyright 2019 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

PY - 2019/3/7

Y1 - 2019/3/7

N2 - The mechanism responsible for the oxidative stress due to photobiomodulation induced by 1265 nm laser is still unclear. Mitochondria are assumed to be the most probable acceptors of the 1265 nm laser irradiation. We study oxidative stress, mitochondrial potential, GSH, cell viability, DNA damage. We demonstrated that narrowband (highcoherent) and wideband lasers employed at the doses of 9.45 and 66.6-400 J/cm2, respectively, induce a dose-dependent cell death, increase ROS level, disturb mitochondrial functioning and can damage DNA. Thus, the 1265 nm lasers can affect the HCT116 cells through mitochondrial damage. Energy density increase contributes to cell damaging without heating effects.

AB - The mechanism responsible for the oxidative stress due to photobiomodulation induced by 1265 nm laser is still unclear. Mitochondria are assumed to be the most probable acceptors of the 1265 nm laser irradiation. We study oxidative stress, mitochondrial potential, GSH, cell viability, DNA damage. We demonstrated that narrowband (highcoherent) and wideband lasers employed at the doses of 9.45 and 66.6-400 J/cm2, respectively, induce a dose-dependent cell death, increase ROS level, disturb mitochondrial functioning and can damage DNA. Thus, the 1265 nm lasers can affect the HCT116 cells through mitochondrial damage. Energy density increase contributes to cell damaging without heating effects.

KW - 1265 nm laser irradiation

KW - DNA damage

KW - cancer cells

KW - near-infrared lasers

KW - oxidative stress

KW - photobiomodulation therapy

KW - reactive oxygen species

KW - singlet oxygen

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U2 - 10.1117/12.2509529

DO - 10.1117/12.2509529

M3 - Conference article

VL - 10861

JO - Proceedings of SPIE - International Society for Optical Engineering

JF - Proceedings of SPIE - International Society for Optical Engineering

SN - 0277-786X

M1 - 108610L

T2 - Mechanisms of Photobiomodulation Therapy XIV

Y2 - 2 February 2019 through 7 February 2019

ER -

Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells

Abstract

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Keywords

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