Abstract
Original language | English |
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Article number | 108610L |
Journal | Proceedings of SPIE - International Society for Optical Engineering |
Volume | 10861 |
DOIs | |
Publication status | Published - 7 Mar 2019 |
Event | Mechanisms of Photobiomodulation Therapy XIV - San Francisco, United States Duration: 2 Feb 2019 → 7 Feb 2019 |
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Bibliographical note
Copyright 2019 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.Keywords
- 1265 nm laser irradiation
- DNA damage
- cancer cells
- near-infrared lasers
- oxidative stress
- photobiomodulation therapy
- reactive oxygen species
- singlet oxygen
Cite this
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Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells. / Khokhlova, Anna; Zolotovskii, Igor; Pogodina, Evgeniia; Saenko, Yuri; Stoliarov, Dmitrii; Vorsina, Svetlana; Fotiadi, Andrei; Liamina, Daria; Sokolovski, Sergei; Rafailov, Edik; Hamblin, Michael R. (Editor); Carroll, James D. (Editor); Arany, Praveen (Editor).
In: Proceedings of SPIE - International Society for Optical Engineering, Vol. 10861, 108610L , 07.03.2019.Research output: Contribution to journal › Conference article
TY - JOUR
T1 - Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells
AU - Khokhlova, Anna
AU - Zolotovskii, Igor
AU - Pogodina, Evgeniia
AU - Saenko, Yuri
AU - Stoliarov, Dmitrii
AU - Vorsina, Svetlana
AU - Fotiadi, Andrei
AU - Liamina, Daria
AU - Sokolovski, Sergei
AU - Rafailov, Edik
A2 - Hamblin, Michael R.
A2 - Carroll, James D.
A2 - Arany, Praveen
N1 - Copyright 2019 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
PY - 2019/3/7
Y1 - 2019/3/7
N2 - The mechanism responsible for the oxidative stress due to photobiomodulation induced by 1265 nm laser is still unclear. Mitochondria are assumed to be the most probable acceptors of the 1265 nm laser irradiation. We study oxidative stress, mitochondrial potential, GSH, cell viability, DNA damage. We demonstrated that narrowband (highcoherent) and wideband lasers employed at the doses of 9.45 and 66.6-400 J/cm2, respectively, induce a dose-dependent cell death, increase ROS level, disturb mitochondrial functioning and can damage DNA. Thus, the 1265 nm lasers can affect the HCT116 cells through mitochondrial damage. Energy density increase contributes to cell damaging without heating effects.
AB - The mechanism responsible for the oxidative stress due to photobiomodulation induced by 1265 nm laser is still unclear. Mitochondria are assumed to be the most probable acceptors of the 1265 nm laser irradiation. We study oxidative stress, mitochondrial potential, GSH, cell viability, DNA damage. We demonstrated that narrowband (highcoherent) and wideband lasers employed at the doses of 9.45 and 66.6-400 J/cm2, respectively, induce a dose-dependent cell death, increase ROS level, disturb mitochondrial functioning and can damage DNA. Thus, the 1265 nm lasers can affect the HCT116 cells through mitochondrial damage. Energy density increase contributes to cell damaging without heating effects.
KW - 1265 nm laser irradiation
KW - DNA damage
KW - cancer cells
KW - near-infrared lasers
KW - oxidative stress
KW - photobiomodulation therapy
KW - reactive oxygen species
KW - singlet oxygen
UR - https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10861/2509529/Effects-of-high-and-low-level-1265-nm-laser-irradiation/10.1117/12.2509529.full
UR - http://www.scopus.com/inward/record.url?scp=85066038374&partnerID=8YFLogxK
U2 - 10.1117/12.2509529
DO - 10.1117/12.2509529
M3 - Conference article
VL - 10861
JO - Proceedings of SPIE - International Society for Optical Engineering
JF - Proceedings of SPIE - International Society for Optical Engineering
SN - 0277-786X
M1 - 108610L
ER -