Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells

Anna Khokhlova, Igor Zolotovskii, Evgeniia Pogodina, Yuri Saenko, Dmitrii Stoliarov, Svetlana Vorsina, Andrei Fotiadi, Daria Liamina, Sergei Sokolovski, Edik Rafailov, Michael R. Hamblin (Editor), James D. Carroll (Editor), Praveen Arany (Editor)

Research output: Contribution to journal › Conference article

Abstract

The mechanism responsible for the oxidative stress due to photobiomodulation induced by 1265 nm laser is still unclear. Mitochondria are assumed to be the most probable acceptors of the 1265 nm laser irradiation. We study oxidative stress, mitochondrial potential, GSH, cell viability, DNA damage. We demonstrated that narrowband (highcoherent) and wideband lasers employed at the doses of 9.45 and 66.6-400 J/cm2, respectively, induce a dose-dependent cell death, increase ROS level, disturb mitochondrial functioning and can damage DNA. Thus, the 1265 nm lasers can affect the HCT116 cells through mitochondrial damage. Energy density increase contributes to cell damaging without heating effects.
Original languageEnglish
Article number108610L
JournalProceedings of SPIE - International Society for Optical Engineering
Volume10861
DOIs
Publication statusPublished - 7 Mar 2019
EventMechanisms of Photobiomodulation Therapy XIV - San Francisco, United States
Duration: 2 Feb 2019 → 7 Feb 2019

Fingerprint

HCT116 Cells
Laser beam effects
Irradiation
Cancer
Lasers
Oxidative stress
cancer
Cells
Oxidative Stress
Laser
irradiation
Damage
Cell
DNA
damage
DNA Damage
lasers
Dose
Neoplasms
Mitochondria

Bibliographical note

Copyright 2019 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Keywords

  • 1265 nm laser irradiation
  • DNA damage
  • cancer cells
  • near-infrared lasers
  • oxidative stress
  • photobiomodulation therapy
  • reactive oxygen species
  • singlet oxygen

Cite this

Khokhlova, A., Zolotovskii, I., Pogodina, E., Saenko, Y., Stoliarov, D., Vorsina, S., ... Arany, P. (Ed.) (2019). Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells. Proceedings of SPIE - International Society for Optical Engineering, 10861, [108610L ]. https://doi.org/10.1117/12.2509529
Khokhlova, Anna ; Zolotovskii, Igor ; Pogodina, Evgeniia ; Saenko, Yuri ; Stoliarov, Dmitrii ; Vorsina, Svetlana ; Fotiadi, Andrei ; Liamina, Daria ; Sokolovski, Sergei ; Rafailov, Edik ; Hamblin, Michael R. (Editor) ; Carroll, James D. (Editor) ; Arany, Praveen (Editor). / Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells. In: Proceedings of SPIE - International Society for Optical Engineering. 2019 ; Vol. 10861.
@article{cfda93b73d8c428d87792340e5fa672b,
title = "Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells",
abstract = "The mechanism responsible for the oxidative stress due to photobiomodulation induced by 1265 nm laser is still unclear. Mitochondria are assumed to be the most probable acceptors of the 1265 nm laser irradiation. We study oxidative stress, mitochondrial potential, GSH, cell viability, DNA damage. We demonstrated that narrowband (highcoherent) and wideband lasers employed at the doses of 9.45 and 66.6-400 J/cm2, respectively, induce a dose-dependent cell death, increase ROS level, disturb mitochondrial functioning and can damage DNA. Thus, the 1265 nm lasers can affect the HCT116 cells through mitochondrial damage. Energy density increase contributes to cell damaging without heating effects.",
keywords = "1265 nm laser irradiation, DNA damage, cancer cells, near-infrared lasers, oxidative stress, photobiomodulation therapy, reactive oxygen species, singlet oxygen",
author = "Anna Khokhlova and Igor Zolotovskii and Evgeniia Pogodina and Yuri Saenko and Dmitrii Stoliarov and Svetlana Vorsina and Andrei Fotiadi and Daria Liamina and Sergei Sokolovski and Edik Rafailov and Hamblin, {Michael R.} and Carroll, {James D.} and Praveen Arany",
note = "Copyright 2019 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.",
year = "2019",
month = "3",
day = "7",
doi = "10.1117/12.2509529",
language = "English",
volume = "10861",
journal = "Proceedings of SPIE - International Society for Optical Engineering",
issn = "0277-786X",
publisher = "SPIE",

}

Khokhlova, A, Zolotovskii, I, Pogodina, E, Saenko, Y, Stoliarov, D, Vorsina, S, Fotiadi, A, Liamina, D, Sokolovski, S, Rafailov, E, Hamblin, MR (ed.), Carroll, JD (ed.) & Arany, P (ed.) 2019, 'Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells', Proceedings of SPIE - International Society for Optical Engineering, vol. 10861, 108610L . https://doi.org/10.1117/12.2509529

Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells. / Khokhlova, Anna; Zolotovskii, Igor; Pogodina, Evgeniia; Saenko, Yuri; Stoliarov, Dmitrii; Vorsina, Svetlana; Fotiadi, Andrei; Liamina, Daria; Sokolovski, Sergei; Rafailov, Edik; Hamblin, Michael R. (Editor); Carroll, James D. (Editor); Arany, Praveen (Editor).

In: Proceedings of SPIE - International Society for Optical Engineering, Vol. 10861, 108610L , 07.03.2019.

Research output: Contribution to journal › Conference article

TY - JOUR

T1 - Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells

AU - Khokhlova, Anna

AU - Zolotovskii, Igor

AU - Pogodina, Evgeniia

AU - Saenko, Yuri

AU - Stoliarov, Dmitrii

AU - Vorsina, Svetlana

AU - Fotiadi, Andrei

AU - Liamina, Daria

AU - Sokolovski, Sergei

AU - Rafailov, Edik

A2 - Hamblin, Michael R.

A2 - Carroll, James D.

A2 - Arany, Praveen

N1 - Copyright 2019 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

PY - 2019/3/7

Y1 - 2019/3/7

N2 - The mechanism responsible for the oxidative stress due to photobiomodulation induced by 1265 nm laser is still unclear. Mitochondria are assumed to be the most probable acceptors of the 1265 nm laser irradiation. We study oxidative stress, mitochondrial potential, GSH, cell viability, DNA damage. We demonstrated that narrowband (highcoherent) and wideband lasers employed at the doses of 9.45 and 66.6-400 J/cm2, respectively, induce a dose-dependent cell death, increase ROS level, disturb mitochondrial functioning and can damage DNA. Thus, the 1265 nm lasers can affect the HCT116 cells through mitochondrial damage. Energy density increase contributes to cell damaging without heating effects.

AB - The mechanism responsible for the oxidative stress due to photobiomodulation induced by 1265 nm laser is still unclear. Mitochondria are assumed to be the most probable acceptors of the 1265 nm laser irradiation. We study oxidative stress, mitochondrial potential, GSH, cell viability, DNA damage. We demonstrated that narrowband (highcoherent) and wideband lasers employed at the doses of 9.45 and 66.6-400 J/cm2, respectively, induce a dose-dependent cell death, increase ROS level, disturb mitochondrial functioning and can damage DNA. Thus, the 1265 nm lasers can affect the HCT116 cells through mitochondrial damage. Energy density increase contributes to cell damaging without heating effects.

KW - 1265 nm laser irradiation

KW - DNA damage

KW - cancer cells

KW - near-infrared lasers

KW - oxidative stress

KW - photobiomodulation therapy

KW - reactive oxygen species

KW - singlet oxygen

UR - https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10861/2509529/Effects-of-high-and-low-level-1265-nm-laser-irradiation/10.1117/12.2509529.full

UR - http://www.scopus.com/inward/record.url?scp=85066038374&partnerID=8YFLogxK

U2 - 10.1117/12.2509529

DO - 10.1117/12.2509529

M3 - Conference article

VL - 10861

JO - Proceedings of SPIE - International Society for Optical Engineering

JF - Proceedings of SPIE - International Society for Optical Engineering

SN - 0277-786X

M1 - 108610L

ER -

Khokhlova A, Zolotovskii I, Pogodina E, Saenko Y, Stoliarov D, Vorsina S et al. Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells. Proceedings of SPIE - International Society for Optical Engineering. 2019 Mar 7;10861. 108610L . https://doi.org/10.1117/12.2509529

Access to Document