Endofluorescence Imaging of Murine Hepatocellular Carcinoma Cell Culture by Fluorescence Lifetime Microscopy with Modulated CMOS Camera

Elena Potapova*, Evgeny Zherebtsov, Ksenia Kandurova, Alexander Palalov, Viktor Dremin, Andrey Dunaev

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

In this work, we propose the results of pilot studies on using the modulated CMOS camera for the imaging of autofluorescence in murine hepatocellular carcinoma cells with excitation in UV light. Here, we assess the capabilities of the imaging system to detect changes in the NAD(P)H fractions produced and utilised in the glycolysis, pentose phosphate pathway and oxidative phosphorylation. Our results suggest that the camera and the system based on one possess a sufficient margin of SNR and sensitivity to detect the cellular metabolic changes associated with the metabolic pathways mentioned.

Original languageEnglish
Article number010303
Number of pages7
JournalJournal of Biomedical Photonics and Engineering
Volume8
Issue number1
DOIs
Publication statusPublished - 22 Mar 2022

Bibliographical note

Copyright The Authors 2022. This work is licensed under a Creative Commons Attribution 4.0 International License
Funding Information:
The authors acknowledge the support of the Russian Science Foundation under project No. 21-15-00325. The authors appreciate the technical support and valuable discussions of Dr. Gerhard Holst (PCO AG, Kelheim, Germany).

Keywords

  • frequency domain fluorescence lifetime imaging
  • hepatocellular carcinoma
  • metabolism

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