In this work, we propose the results of pilot studies on using the modulated CMOS camera for the imaging of autofluorescence in murine hepatocellular carcinoma cells with excitation in UV light. Here, we assess the capabilities of the imaging system to detect changes in the NAD(P)H fractions produced and utilised in the glycolysis, pentose phosphate pathway and oxidative phosphorylation. Our results suggest that the camera and the system based on one possess a sufficient margin of SNR and sensitivity to detect the cellular metabolic changes associated with the metabolic pathways mentioned.
|Number of pages||7|
|Journal||Journal of Biomedical Photonics and Engineering|
|Publication status||Published - 22 Mar 2022|
Bibliographical noteCopyright The Authors 2022. This work is licensed under a Creative Commons Attribution 4.0 International License
The authors acknowledge the support of the Russian Science Foundation under project No. 21-15-00325. The authors appreciate the technical support and valuable discussions of Dr. Gerhard Holst (PCO AG, Kelheim, Germany).
- frequency domain fluorescence lifetime imaging
- hepatocellular carcinoma