Fluorescence spectroscopy approach for blood influence compensation

Valery Shupletsov*, Viktor Dremin, Evgeny Zherebtsov, Mikhail Mezentsev, Igor Kozlov, Elena Potapova, Andrey Dunaev

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review


At present, fluorescence spectroscopy (FS) and diffuse reflectance spectroscopy (DRS) are widespread methods highly used in medical practice. The combined application of these methods is a promising tool to improve the predictive force of classifiers for tissue type recognition as well as to compensate the attenuation of the fluorescence radiation by blood for the accurate evaluation of the biomarkers content in living tissue. Several techniques are known to normalize the resulting fluorescence spectrum in order to exclude the attenuation effect. In this study, an approach based on the dividing of the experimentally obtained FS spectrum by DRS spectrum has been applied for experiments with occlusion test. The implemented multimodal approach for the in vivo optical measurements in combination with occlusion test for minimisation of blood influence has shown good repeatability of obtained experimental fluorescence spectra. The results are of particular interest for the further development of methods for compensating the influence of chromophores in optical spectroscopy.

Original languageEnglish
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Publication statusPublished - 3 Jun 2019
EventSaratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine, SFM 2018 - Saratov, Russian Federation
Duration: 24 Sept 201828 Sept 2018

Bibliographical note

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  • Blood absorption
  • Blood compensation
  • Diffuse reflectance spectroscopy
  • Fluorescence spectroscopy


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