c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE

Alan D. Goddard, Julie M. Stevens, Feng Rao, Despoina A.I. Mavridou, Weelee Chan, David J. Richardson, James W.A. Allen, Stuart J. Ferguson

Research output: Contribution to journalArticle

Abstract

The Ccm cytochrome c maturation System I catalyzes covalent attachment of heme to apocytochromes c in many bacterial species and some mitochondria. A covalent, but transient, bond between heme and a conserved histidine in CcmE along with an interaction between CcmH and the apocytochrome have been previously indicated as core aspects of the Ccm system. Here, we show that in the Ccm system from Desulfovibrio desulfuricans, no CcmH is required, and the holo-CcmE covalent bond occurs via a cysteine residue. These observations call for reconsideration of the accepted models of System I-mediated c-type cytochrome biogenesis.

LanguageEnglish
Pages22882-22889
Number of pages8
JournalJournal of Biological Chemistry
Volume285
Issue number30
Early online date13 May 2010
DOIs
Publication statusPublished - 23 Jul 2010

Fingerprint

Desulfovibrio desulfuricans
Cytochrome c Group
Mitochondria
Covalent bonds
Cytochromes c
Heme
Histidine
Cysteine
heme a

Bibliographical note

© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

Errata: Goddard, A. D., Stevens, J. M., Rao, F., Mavridou, D. A. I., Chan, W., Richardson, D. J., ... Ferguson, S. J. (2011). c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE. Journal of Biological Chemistry, 286(33), 29442. DOI: 10.1074/jbc.A110.133421

Keywords

  • chaperone chaperonin
  • cytochrome c
  • cytochromes
  • heme
  • post-translational modification
  • protein assembly
  • cytochrome c biogenesis
  • cytochrome c maturation
  • heme chaperone

Cite this

Goddard, Alan D. ; Stevens, Julie M. ; Rao, Feng ; Mavridou, Despoina A.I. ; Chan, Weelee ; Richardson, David J. ; Allen, James W.A. ; Ferguson, Stuart J. / c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 30. pp. 22882-22889.
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c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE. / Goddard, Alan D.; Stevens, Julie M.; Rao, Feng; Mavridou, Despoina A.I.; Chan, Weelee; Richardson, David J.; Allen, James W.A.; Ferguson, Stuart J.

In: Journal of Biological Chemistry, Vol. 285, No. 30, 23.07.2010, p. 22882-22889.

Research output: Contribution to journalArticle

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