Abstract
A method is described for the introduction of structural diversity into the thiocarbonyl group of 6-thioguanine within support-bound, fully protected oligonucleotides via 'on-column′ conjugation. 2′-Deoxy-6-thioguanosine with a chemically-labile trigger at its 6-thio function was incorporated at defined sites into chemically synthesized oligonucleotides. Following selective removal of the thio-protection group the support-immobilized oligonucleotides were conjugated with various groups on-column, and then deprotected and purified to produce a number of oligomers each containing a different modified base. Since the modification is accomplished on-column without affecting other functional and protecting groups in the oligomers this method is compatible with introducing structural diversity at multiple sites in DNA. © 2003 Elsevier Science Ltd. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 1925-1932 |
| Number of pages | 8 |
| Journal | Tetrahedron |
| Volume | 59 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 10 Mar 2003 |
Keywords
- DNA synthesis
- modified base
- modified DNA
- post-synthetic modification
- thioguanine