Isolation and culture of primary rat adipose derived stem cells using porous biopolymer microcarriers

Guang-Zhen Jin, Jeong-Hui Park, Ivan Wall, Hae-Won Kim

Research output: Contribution to journalArticle

Abstract

Adipose-derived stem cells (ADSCs) are an attractive source of material for mesenchymal stem cell research due to the abundance of adipose and relative ease of access compared with bone marrow. A key consideration for research is whether cell isolation methods can be improved, to reduce the process steps needed to isolate and expand cell material. In the current study, we used macroporous biopolymer microcarriers to isolate primary ADSCs. We found that the method was capable of isolating ADSCs that were subsequently capable of being transferred to culture dishes and expanded in vitro. Moreover, flow cytometry revealed that they expressed typical stem cell markers and were capable of undergoing tri-lineage differentiation. In summary, it is feasible to use biopolymer microcarriers for retrieval of viable ADSCs that retain identity markers of stem cell function.

Original languageEnglish
Pages (from-to)242-250
Number of pages9
JournalTissue engineering and regenerative medicine
Volume13
Issue number3
DOIs
Publication statusPublished - 9 Jun 2016

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Biopolymers
Stem cells
Cell culture
Rats
Stem Cells
Stem Cell Research
Cell Separation
Flow cytometry
Mesenchymal Stromal Cells
Flow Cytometry
Bone Marrow
Bone
Research

Cite this

Jin, G-Z., Park, J-H., Wall, I., & Kim, H-W. (2016). Isolation and culture of primary rat adipose derived stem cells using porous biopolymer microcarriers. Tissue engineering and regenerative medicine, 13(3), 242-250. https://doi.org/10.1007/s13770-016-0040-z
Jin, Guang-Zhen ; Park, Jeong-Hui ; Wall, Ivan ; Kim, Hae-Won. / Isolation and culture of primary rat adipose derived stem cells using porous biopolymer microcarriers. In: Tissue engineering and regenerative medicine. 2016 ; Vol. 13, No. 3. pp. 242-250.
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Jin, G-Z, Park, J-H, Wall, I & Kim, H-W 2016, 'Isolation and culture of primary rat adipose derived stem cells using porous biopolymer microcarriers', Tissue engineering and regenerative medicine, vol. 13, no. 3, pp. 242-250. https://doi.org/10.1007/s13770-016-0040-z

Isolation and culture of primary rat adipose derived stem cells using porous biopolymer microcarriers. / Jin, Guang-Zhen; Park, Jeong-Hui; Wall, Ivan; Kim, Hae-Won.

In: Tissue engineering and regenerative medicine, Vol. 13, No. 3, 09.06.2016, p. 242-250.

Research output: Contribution to journalArticle

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T1 - Isolation and culture of primary rat adipose derived stem cells using porous biopolymer microcarriers

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AB - Adipose-derived stem cells (ADSCs) are an attractive source of material for mesenchymal stem cell research due to the abundance of adipose and relative ease of access compared with bone marrow. A key consideration for research is whether cell isolation methods can be improved, to reduce the process steps needed to isolate and expand cell material. In the current study, we used macroporous biopolymer microcarriers to isolate primary ADSCs. We found that the method was capable of isolating ADSCs that were subsequently capable of being transferred to culture dishes and expanded in vitro. Moreover, flow cytometry revealed that they expressed typical stem cell markers and were capable of undergoing tri-lineage differentiation. In summary, it is feasible to use biopolymer microcarriers for retrieval of viable ADSCs that retain identity markers of stem cell function.

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Jin G-Z, Park J-H, Wall I, Kim H-W. Isolation and culture of primary rat adipose derived stem cells using porous biopolymer microcarriers. Tissue engineering and regenerative medicine. 2016 Jun 9;13(3):242-250. https://doi.org/10.1007/s13770-016-0040-z