Label-free immunoassay for porcine circovirus type 2 based on excessively tilted fiber grating modified with staphylococcal protein A

Binbin Luo, Shengxi Wu*, Wengen Zou, Zhonghao Zhang, Mingfu Zhao, Yong Liu, Shenghui Shi, Xiangfeng Xi, Zheng Zeng, Wangwang Liang, Zhijun Yan, Lin Zhang

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Using excessively tilted fiber grating (Ex-TFG) inscribed in standard single mode fiber, we developed a novel label-free immunoassay for specific detection of porcine circovirus type 2 (PCV2), which is a minim animal virus. Staphylococcal protein A (SPA) was used to modify the silanized fiber surface thus forming a SPA layer, which would greatly enhance the proportion of anti-PCV2 monoclonal antibody (MAb) bioactivity, thus improving the effectiveness of specific adsorption and binding events between anti-PCV2 MAbs and PCV2 antigens. Immunoassay experiments were carried out by monitoring the resonance wavelength shift of the proposed sensor under different PCV2 titer levels. Anti-PCV2 MAbs were thoroughly dissociated from the SPA layer by treatment with urea, and recombined to the SPA layer on the sensor surface for repeated immunoassay of PCV2. The specificity of the immunosensor was inspected by detecting porcine reproductive and respiratory syndrome virus (PRRSV) first, and PCV2 subsequently. The results showed a limit of detection (LOD) for the PCV2 immunosensor of ~9.371TCID50/mL, for a saturation value of ~4.801×103TCID50/mL, with good repeatability and excellent specificity.
Original languageEnglish
Pages (from-to)1054-1060
Number of pages7
JournalBiosensors and Bioelectronics
Volume86
Early online date29 Jul 2016
DOIs
Publication statusPublished - 15 Dec 2016

Bibliographical note

© 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

  • excessively tilted fiber grating (Ex-TFG)
  • label-free immunoassay
  • monoclonal antibody (MAb)
  • porcine circovirus type 2 (PCV2)
  • staphylococcal protein A (SPA)

Fingerprint Dive into the research topics of 'Label-free immunoassay for porcine circovirus type 2 based on excessively tilted fiber grating modified with staphylococcal protein A'. Together they form a unique fingerprint.

  • Cite this

    Luo, B., Wu, S., Zou, W., Zhang, Z., Zhao, M., Liu, Y., Shi, S., Xi, X., Zeng, Z., Liang, W., Yan, Z., & Zhang, L. (2016). Label-free immunoassay for porcine circovirus type 2 based on excessively tilted fiber grating modified with staphylococcal protein A. Biosensors and Bioelectronics, 86, 1054-1060. https://doi.org/10.1016/j.bios.2016.07.100