Biopharmaceuticals are commonly present in relatively low concentrations in aqueous solutions, making their detection and purification detrimental. In this work, we used novel aqueous biphasic systems based on glycerol formal (GF) to extract an important antibiotic - tetracycline. We report cloud points (solubility curve) and tie-lines for three ternary systems, containing GF, water, and inorganic salt (either K3PO4, K2HPO4, or K2CO3) at constant temperature of 298 K and at 0.1 MPa. The tie-line data of these ternary systems were correlated using the nonrandom two-liquid model, and binary interaction parameters of activity coefficients were estimated. The experimental and correlated tie-line data were compared in terms of average root-mean-square deviation and showed satisfactory agreements. The partition coefficients of tetracycline between two phases were measured, and corresponding extraction efficiencies were calculated. The maximum value of partition coefficient was 1551 for the system containing K3PO4, followed by values of 1145 and 927 for systems containing K2CO3 and K2HPO4, respectively. The calculated extraction efficiencies were very high - greater than 98.8%, demonstrating high potential for using aqueous biphasic systems based on GF for separation and purification processes.
Bibliographical noteThis document is the Accepted Manuscript version of a Published Work that appeared in final form in J. Chem. Eng. Data, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jced.9b00376
Funding: Thailand Research Fund (Research and Researchers for Industrials PhD Program: Grant No. PHD 57I0078), the Joy Welch Educational Charitable Trust (Lancaster University 2018), and the “Research Chair Grant” National Science and Technology Development Agency (NSTDA).