The use of human mesenchymal stem cells (hMSCs) in regenerative medicine is a potential major advance for the treatment of many medical conditions, especially with the use of allogeneic therapies where the cells from a single donor can be used to treat ailments in many patients. Such cells must be grown attached to surfaces and for large scale production, it is shown that stirred bioreactors containing ~200 μm particles (microcarriers) can provide such a surface. It is also shown that the just suspended condition, agitator speed NJS, provides a satisfactory condition for cell growth by minimizing the specific energy dissipation rate, εT, in the bioreactor whilst still meeting the oxygen demand of the cells. For the cells to be used for therapeutic purposes, they must be detached from the microcarriers before being cryopreserved. A strategy based on a short period (~7 min) of very high εT, based on theories of secondary nucleation, is effective at removing >99% cells. Once removed, the cells are smaller than the Kolmogorov scale of turbulence and hence not damaged. This approach is shown to be successful for culture and detachment in 4 types of stirred bioreactors from 15 mL to 5 L.
|Number of pages||6|
|Journal||Theoretical Foundations of Chemical Engineering|
|Early online date||2 Dec 2016|
|Publication status||E-pub ahead of print - 2 Dec 2016|
|Event||15th European Conference on Mixing - St. Petersburg, Russian Federation|
Duration: 25 Jan 2015 → 3 Jul 2015
Bibliographical noteThe final publication is available at Springer via http://dx.doi.org/10.1134/S0040579516060117
Proceedings of XXV European Conference on Mixing “MIXING 15”
Funding: EPSRC, Lonza Cologne AG and FujiFilm Diosynth Biotechnologies.
- mesenchymal stem cells