Modified titanium surfaces promote accelerated osteogenic differentiation of mesenchymal stromal cells in vitro

Ivan Wall, Nikos Donos, Karin Carlqvist, Francis Jones, Peter Brett

Research output: Contribution to journalArticle

Abstract

Titanium (Ti) is the material of choice for dental and orthopaedic implants due to its highly biocompatible nature. Modification of the implant surface, either topographically (as roughness) or chemically, can promote accelerated osteogenesis in vivo and greatly increase bone-implant contact and bonding strength. In this paper, we sought to characterise the cellular and molecular responses of human bone marrow-derived mesenchymal stromal cells (hMSCs) to two modified Ti surfaces: a rough hydrophobic surface that was sand-blasted and acid-etched (SLA) and an SLA surface of the same roughness that was chemically modified to have high wettability/hydrophilicity (SLActive). A smooth polished (SMO) Ti surface was used as a control. Whilst no differences in initial cell attachment to any of the surfaces were observed, we found that hMSCs cultured on the rough surfaces underwent a decrease in cell number early in culture, yet simultaneously expressed higher levels of the osteogenic markers SPP1, RUNX2 and BSP. Furthermore, deposits of calcified matrix were observed at earlier time points on both SLA and SLActive surfaces compared to SMO and this correlated with increased expression of the osteogenic promoter WNT5A in response to the rough surfaces. Osteogenic responses to SLActive were moderately better than the hydrophobic SLA surface and gene expression studies indicate that WNT5A activation may be responsible for this increased osteogenic differentiation.

Original languageEnglish
Pages (from-to)17-26
Number of pages10
JournalBone
Volume45
Issue number1
DOIs
Publication statusPublished - Jul 2009

Keywords

  • Anthraquinones
  • Biomarkers/metabolism
  • Calcification, Physiologic/drug effects
  • Cell Adhesion/drug effects
  • Cell Death/drug effects
  • Cell Differentiation/drug effects
  • Cell Lineage/drug effects
  • Cell Proliferation/drug effects
  • Cells, Cultured
  • Extracellular Matrix/drug effects
  • Gene Expression Profiling
  • Humans
  • Mesoderm/cytology
  • Osteogenesis/drug effects
  • Staining and Labeling
  • Stromal Cells/cytology
  • Surface Properties/drug effects
  • Titanium/pharmacology
  • Transcription, Genetic/drug effects
  • Wettability/drug effects

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