Nanophotonic biosensors within lab on chip optical systems

Daniel Hill*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

For ring resonator based sensors, volumetric limits of detection (LoD) of 5×10-6 RIU and 8.3×10-6 RIU (refractive index units) for sensitivities of 246nm/RIU and 2169nm/RIU were reported from FP6 SABIO (at 1.31μm) and FP7 InTopSens (at 1.55μm) respectively. These compare well to the state of art of 7.6×10-7 RIU for a sensitivity of 163 nm/RIU, as does the porous alumina based membrane sensors in FP7 Positive with their LoD of 5×10-6 RIU. More interestingly for the membrane sensors, the standard deviation of their measured values was below 5% and their flow through design with lateral distances to the sensor surface less than a diffusion length permit fast response times, short assay times and the use of small sample volumes (< 100 μl). For protein binding recognition, within SABIO a surface LoD of 0.9 pg/mm2 for anti-BSA on a gluteraldehyde-covered surface was recorded, corresponding to a 125ng/ml anti-BSA solution, whilst in InTopSens 5pg/mm2 and 10ng/ml for biotin on a streptavidin coated surface was seen. For an assay of β-lactoglobulin - anti-P-lactoglobulin - anti-rabbit-IgG-streptavidin conjugated CdSe quantum dots the Positive sensors demonstrated a noise floor for individual measurements of 3.7ng/ml (25pM) for total assay times of under one hour.

Original languageEnglish
Title of host publicationPHOTOPTICS 2015 - 3rd International Conference on Photonics, Optics and Laser Technology, Proceedings
EditorsPaulo A. Ribeiro, Maria Raposo
PublisherSciTePress
Pages60-68
Number of pages9
Volume2
ISBN (Electronic)9789897580932
Publication statusPublished - 1 Jan 2015
Event3rd International Conference on Photonics, Optics and Laser Technology, PHOTOPTICS 2015 - Berlin, Germany
Duration: 12 Mar 201514 Mar 2015

Conference

Conference3rd International Conference on Photonics, Optics and Laser Technology, PHOTOPTICS 2015
CountryGermany
CityBerlin
Period12/03/1514/03/15

Fingerprint

bioinstrumentation
chips
refractivity
sensors
membranes
biotin
sensitivity
rabbits
diffusion length
standard deviation
aluminum oxides
resonators
quantum dots
proteins
rings

Keywords

  • Biosensing
  • Birefringence
  • Lab-on-chip
  • Nanophotonics
  • Porous silicon
  • Quantum dots
  • Ring resonators
  • Slot-waveguides

Cite this

Hill, D. (2015). Nanophotonic biosensors within lab on chip optical systems. In P. A. Ribeiro, & M. Raposo (Eds.), PHOTOPTICS 2015 - 3rd International Conference on Photonics, Optics and Laser Technology, Proceedings (Vol. 2, pp. 60-68). SciTePress.
Hill, Daniel. / Nanophotonic biosensors within lab on chip optical systems. PHOTOPTICS 2015 - 3rd International Conference on Photonics, Optics and Laser Technology, Proceedings. editor / Paulo A. Ribeiro ; Maria Raposo. Vol. 2 SciTePress, 2015. pp. 60-68
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abstract = "For ring resonator based sensors, volumetric limits of detection (LoD) of 5×10-6 RIU and 8.3×10-6 RIU (refractive index units) for sensitivities of 246nm/RIU and 2169nm/RIU were reported from FP6 SABIO (at 1.31μm) and FP7 InTopSens (at 1.55μm) respectively. These compare well to the state of art of 7.6×10-7 RIU for a sensitivity of 163 nm/RIU, as does the porous alumina based membrane sensors in FP7 Positive with their LoD of 5×10-6 RIU. More interestingly for the membrane sensors, the standard deviation of their measured values was below 5{\%} and their flow through design with lateral distances to the sensor surface less than a diffusion length permit fast response times, short assay times and the use of small sample volumes (< 100 μl). For protein binding recognition, within SABIO a surface LoD of 0.9 pg/mm2 for anti-BSA on a gluteraldehyde-covered surface was recorded, corresponding to a 125ng/ml anti-BSA solution, whilst in InTopSens 5pg/mm2 and 10ng/ml for biotin on a streptavidin coated surface was seen. For an assay of β-lactoglobulin - anti-P-lactoglobulin - anti-rabbit-IgG-streptavidin conjugated CdSe quantum dots the Positive sensors demonstrated a noise floor for individual measurements of 3.7ng/ml (25pM) for total assay times of under one hour.",
keywords = "Biosensing, Birefringence, Lab-on-chip, Nanophotonics, Porous silicon, Quantum dots, Ring resonators, Slot-waveguides",
author = "Daniel Hill",
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Hill, D 2015, Nanophotonic biosensors within lab on chip optical systems. in PA Ribeiro & M Raposo (eds), PHOTOPTICS 2015 - 3rd International Conference on Photonics, Optics and Laser Technology, Proceedings. vol. 2, SciTePress, pp. 60-68, 3rd International Conference on Photonics, Optics and Laser Technology, PHOTOPTICS 2015, Berlin, Germany, 12/03/15.

Nanophotonic biosensors within lab on chip optical systems. / Hill, Daniel.

PHOTOPTICS 2015 - 3rd International Conference on Photonics, Optics and Laser Technology, Proceedings. ed. / Paulo A. Ribeiro; Maria Raposo. Vol. 2 SciTePress, 2015. p. 60-68.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Hill, Daniel

PY - 2015/1/1

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N2 - For ring resonator based sensors, volumetric limits of detection (LoD) of 5×10-6 RIU and 8.3×10-6 RIU (refractive index units) for sensitivities of 246nm/RIU and 2169nm/RIU were reported from FP6 SABIO (at 1.31μm) and FP7 InTopSens (at 1.55μm) respectively. These compare well to the state of art of 7.6×10-7 RIU for a sensitivity of 163 nm/RIU, as does the porous alumina based membrane sensors in FP7 Positive with their LoD of 5×10-6 RIU. More interestingly for the membrane sensors, the standard deviation of their measured values was below 5% and their flow through design with lateral distances to the sensor surface less than a diffusion length permit fast response times, short assay times and the use of small sample volumes (< 100 μl). For protein binding recognition, within SABIO a surface LoD of 0.9 pg/mm2 for anti-BSA on a gluteraldehyde-covered surface was recorded, corresponding to a 125ng/ml anti-BSA solution, whilst in InTopSens 5pg/mm2 and 10ng/ml for biotin on a streptavidin coated surface was seen. For an assay of β-lactoglobulin - anti-P-lactoglobulin - anti-rabbit-IgG-streptavidin conjugated CdSe quantum dots the Positive sensors demonstrated a noise floor for individual measurements of 3.7ng/ml (25pM) for total assay times of under one hour.

AB - For ring resonator based sensors, volumetric limits of detection (LoD) of 5×10-6 RIU and 8.3×10-6 RIU (refractive index units) for sensitivities of 246nm/RIU and 2169nm/RIU were reported from FP6 SABIO (at 1.31μm) and FP7 InTopSens (at 1.55μm) respectively. These compare well to the state of art of 7.6×10-7 RIU for a sensitivity of 163 nm/RIU, as does the porous alumina based membrane sensors in FP7 Positive with their LoD of 5×10-6 RIU. More interestingly for the membrane sensors, the standard deviation of their measured values was below 5% and their flow through design with lateral distances to the sensor surface less than a diffusion length permit fast response times, short assay times and the use of small sample volumes (< 100 μl). For protein binding recognition, within SABIO a surface LoD of 0.9 pg/mm2 for anti-BSA on a gluteraldehyde-covered surface was recorded, corresponding to a 125ng/ml anti-BSA solution, whilst in InTopSens 5pg/mm2 and 10ng/ml for biotin on a streptavidin coated surface was seen. For an assay of β-lactoglobulin - anti-P-lactoglobulin - anti-rabbit-IgG-streptavidin conjugated CdSe quantum dots the Positive sensors demonstrated a noise floor for individual measurements of 3.7ng/ml (25pM) for total assay times of under one hour.

KW - Biosensing

KW - Birefringence

KW - Lab-on-chip

KW - Nanophotonics

KW - Porous silicon

KW - Quantum dots

KW - Ring resonators

KW - Slot-waveguides

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M3 - Conference contribution

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VL - 2

SP - 60

EP - 68

BT - PHOTOPTICS 2015 - 3rd International Conference on Photonics, Optics and Laser Technology, Proceedings

A2 - Ribeiro, Paulo A.

A2 - Raposo, Maria

PB - SciTePress

ER -

Hill D. Nanophotonic biosensors within lab on chip optical systems. In Ribeiro PA, Raposo M, editors, PHOTOPTICS 2015 - 3rd International Conference on Photonics, Optics and Laser Technology, Proceedings. Vol. 2. SciTePress. 2015. p. 60-68