TY - JOUR
T1 - Optimization of random amplification of polymorphic DNA analysis for molecular subtyping of Escherichia coli O157
AU - Hopkins, K. L.
AU - Hilton, A. C.
PY - 2001/3
Y1 - 2001/3
N2 - Random amplification of polymorphic DNA (RAPD) analysis using the polymerase chain reaction has proved to be a useful technique in the epidemiological investigation of micro-organisms but may suffer from a lack of reproducibility in poorly optimized protocols. In this study a method of obtaining reproducible genomic fingerprints using RAPD analysis of Escherichia coli O157 is described. By systematic optimization of reaction conditions and selection of suitable primers, reproducible and discriminatory profiles could be obtained from all E. coli O157 strains tested. In addition, two other methods of obtaining reproducible profiles from E. coli O157 strains without the need to purify genomic DNA are described.
AB - Random amplification of polymorphic DNA (RAPD) analysis using the polymerase chain reaction has proved to be a useful technique in the epidemiological investigation of micro-organisms but may suffer from a lack of reproducibility in poorly optimized protocols. In this study a method of obtaining reproducible genomic fingerprints using RAPD analysis of Escherichia coli O157 is described. By systematic optimization of reaction conditions and selection of suitable primers, reproducible and discriminatory profiles could be obtained from all E. coli O157 strains tested. In addition, two other methods of obtaining reproducible profiles from E. coli O157 strains without the need to purify genomic DNA are described.
UR - http://www.scopus.com/inward/record.url?scp=0035108088&partnerID=8YFLogxK
UR - https://onlinelibrary.wiley.com/doi/full/10.1046/j.1472-765x.2001.00871.x?sid=nlm%3Apubmed
U2 - 10.1046/j.1472-765x.2001.00871.x
DO - 10.1046/j.1472-765x.2001.00871.x
M3 - Article
C2 - 11264738
SN - 0266-8254
VL - 32
SP - 126
EP - 130
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
IS - 3
ER -