Production and characterization of monoclonal antibodies against the leukemia inhibitory factor low affinity receptor, gp190

V Pitard, J L Taupin, V Miossec, F Blanchard, M Cransac, I Jollet, A Vernallis, K Hudson, A Godard, Y Jacques, J F Moreau

Research output: Contribution to journalArticle

Abstract

Leukemia inhibitory factor (LIF), oncostatin-M (OSM), ciliary neurotrophic factor (CNTF) and cardiotrophin-1 (CT1) act through transmembrane receptors which share the gp190 glycoprotein chain. The understanding of its involvement in the biology of these cytokines is of importance since these systems have recently been shown to participate in major inflammatory and neoplastic processes such as myelomatosis (Rose-John, S., Heinrich, P.C., 1994. Soluble receptors for cytokines and growth factors: generation and biological function. Biochem. J. 300, 281). In addition, this family of receptors also shares the gp130 transducing chain, with the IL6 and IL11 receptors. Because IL6 and gp130 were the first members to be discovered, most of the available reagents are directed at them. In this respect, monoclonal antibodies have played a major role in elucidating these receptor/ligand interactions and exploring the pathophysiological aspects of their biology. So far, no such reagents have been described for the gp190. We now report the production and characterization of 16 monoclonal antibodies directed against human gp190. They were obtained using recombinant chimeric or truncated proteins produced in a eukaryotic CHO cell line. One was able to block the biological activity of LIF. Because gp190 comprises two hematopoietin binding domains, crude epitope mapping was possible using the same reagents. While more of these antibodies are required, the present set validate the technological approach used for their preparation and should improve our understanding of this class of cytokines.
Original languageEnglish
Pages (from-to)177-190
Number of pages14
JournalJournal of Immunological Methods
Volume205
Issue number2
DOIs
Publication statusPublished - Jul 1997

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Leukemia Inhibitory Factor
Monoclonal Antibodies
Interleukin-11 Receptors
Hematopoietic Cell Growth Factors
Oncostatin M
Cytokines
Ciliary Neurotrophic Factor
Epitope Mapping
Neoplastic Processes
Interleukin-6 Receptors
Growth Factor Receptors
CHO Cells
Eukaryotic Cells
Multiple Myeloma
Interleukin-6
Glycoproteins
Ligands
Cell Line
Antibodies
Proteins

Keywords

  • Animals
  • Antibodies, Blocking
  • Antibodies, Monoclonal
  • Base Sequence
  • CHO Cells
  • Cricetinae
  • DNA Primers
  • Growth Inhibitors
  • Humans
  • Immunization
  • Interleukin-6
  • Leukemia Inhibitory Factor
  • Leukemia Inhibitory Factor Receptor alpha Subunit
  • Lymphokines
  • Mice
  • Mice, Inbred BALB C
  • Polymerase Chain Reaction
  • Receptors, Cytokine
  • Receptors, OSM-LIF
  • Recombinant Fusion Proteins

Cite this

Pitard, V ; Taupin, J L ; Miossec, V ; Blanchard, F ; Cransac, M ; Jollet, I ; Vernallis, A ; Hudson, K ; Godard, A ; Jacques, Y ; Moreau, J F. / Production and characterization of monoclonal antibodies against the leukemia inhibitory factor low affinity receptor, gp190. In: Journal of Immunological Methods. 1997 ; Vol. 205, No. 2. pp. 177-190.
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title = "Production and characterization of monoclonal antibodies against the leukemia inhibitory factor low affinity receptor, gp190",
abstract = "Leukemia inhibitory factor (LIF), oncostatin-M (OSM), ciliary neurotrophic factor (CNTF) and cardiotrophin-1 (CT1) act through transmembrane receptors which share the gp190 glycoprotein chain. The understanding of its involvement in the biology of these cytokines is of importance since these systems have recently been shown to participate in major inflammatory and neoplastic processes such as myelomatosis (Rose-John, S., Heinrich, P.C., 1994. Soluble receptors for cytokines and growth factors: generation and biological function. Biochem. J. 300, 281). In addition, this family of receptors also shares the gp130 transducing chain, with the IL6 and IL11 receptors. Because IL6 and gp130 were the first members to be discovered, most of the available reagents are directed at them. In this respect, monoclonal antibodies have played a major role in elucidating these receptor/ligand interactions and exploring the pathophysiological aspects of their biology. So far, no such reagents have been described for the gp190. We now report the production and characterization of 16 monoclonal antibodies directed against human gp190. They were obtained using recombinant chimeric or truncated proteins produced in a eukaryotic CHO cell line. One was able to block the biological activity of LIF. Because gp190 comprises two hematopoietin binding domains, crude epitope mapping was possible using the same reagents. While more of these antibodies are required, the present set validate the technological approach used for their preparation and should improve our understanding of this class of cytokines.",
keywords = "Animals, Antibodies, Blocking, Antibodies, Monoclonal, Base Sequence, CHO Cells, Cricetinae, DNA Primers, Growth Inhibitors, Humans, Immunization, Interleukin-6, Leukemia Inhibitory Factor, Leukemia Inhibitory Factor Receptor alpha Subunit, Lymphokines, Mice, Mice, Inbred BALB C, Polymerase Chain Reaction, Receptors, Cytokine, Receptors, OSM-LIF, Recombinant Fusion Proteins",
author = "V Pitard and Taupin, {J L} and V Miossec and F Blanchard and M Cransac and I Jollet and A Vernallis and K Hudson and A Godard and Y Jacques and Moreau, {J F}",
year = "1997",
month = "7",
doi = "10.1016/S0022-1759(97)00074-4",
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Pitard, V, Taupin, JL, Miossec, V, Blanchard, F, Cransac, M, Jollet, I, Vernallis, A, Hudson, K, Godard, A, Jacques, Y & Moreau, JF 1997, 'Production and characterization of monoclonal antibodies against the leukemia inhibitory factor low affinity receptor, gp190', Journal of Immunological Methods, vol. 205, no. 2, pp. 177-190. https://doi.org/10.1016/S0022-1759(97)00074-4

Production and characterization of monoclonal antibodies against the leukemia inhibitory factor low affinity receptor, gp190. / Pitard, V; Taupin, J L; Miossec, V; Blanchard, F; Cransac, M; Jollet, I; Vernallis, A; Hudson, K; Godard, A; Jacques, Y; Moreau, J F.

In: Journal of Immunological Methods, Vol. 205, No. 2, 07.1997, p. 177-190.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Production and characterization of monoclonal antibodies against the leukemia inhibitory factor low affinity receptor, gp190

AU - Pitard, V

AU - Taupin, J L

AU - Miossec, V

AU - Blanchard, F

AU - Cransac, M

AU - Jollet, I

AU - Vernallis, A

AU - Hudson, K

AU - Godard, A

AU - Jacques, Y

AU - Moreau, J F

PY - 1997/7

Y1 - 1997/7

N2 - Leukemia inhibitory factor (LIF), oncostatin-M (OSM), ciliary neurotrophic factor (CNTF) and cardiotrophin-1 (CT1) act through transmembrane receptors which share the gp190 glycoprotein chain. The understanding of its involvement in the biology of these cytokines is of importance since these systems have recently been shown to participate in major inflammatory and neoplastic processes such as myelomatosis (Rose-John, S., Heinrich, P.C., 1994. Soluble receptors for cytokines and growth factors: generation and biological function. Biochem. J. 300, 281). In addition, this family of receptors also shares the gp130 transducing chain, with the IL6 and IL11 receptors. Because IL6 and gp130 were the first members to be discovered, most of the available reagents are directed at them. In this respect, monoclonal antibodies have played a major role in elucidating these receptor/ligand interactions and exploring the pathophysiological aspects of their biology. So far, no such reagents have been described for the gp190. We now report the production and characterization of 16 monoclonal antibodies directed against human gp190. They were obtained using recombinant chimeric or truncated proteins produced in a eukaryotic CHO cell line. One was able to block the biological activity of LIF. Because gp190 comprises two hematopoietin binding domains, crude epitope mapping was possible using the same reagents. While more of these antibodies are required, the present set validate the technological approach used for their preparation and should improve our understanding of this class of cytokines.

AB - Leukemia inhibitory factor (LIF), oncostatin-M (OSM), ciliary neurotrophic factor (CNTF) and cardiotrophin-1 (CT1) act through transmembrane receptors which share the gp190 glycoprotein chain. The understanding of its involvement in the biology of these cytokines is of importance since these systems have recently been shown to participate in major inflammatory and neoplastic processes such as myelomatosis (Rose-John, S., Heinrich, P.C., 1994. Soluble receptors for cytokines and growth factors: generation and biological function. Biochem. J. 300, 281). In addition, this family of receptors also shares the gp130 transducing chain, with the IL6 and IL11 receptors. Because IL6 and gp130 were the first members to be discovered, most of the available reagents are directed at them. In this respect, monoclonal antibodies have played a major role in elucidating these receptor/ligand interactions and exploring the pathophysiological aspects of their biology. So far, no such reagents have been described for the gp190. We now report the production and characterization of 16 monoclonal antibodies directed against human gp190. They were obtained using recombinant chimeric or truncated proteins produced in a eukaryotic CHO cell line. One was able to block the biological activity of LIF. Because gp190 comprises two hematopoietin binding domains, crude epitope mapping was possible using the same reagents. While more of these antibodies are required, the present set validate the technological approach used for their preparation and should improve our understanding of this class of cytokines.

KW - Animals

KW - Antibodies, Blocking

KW - Antibodies, Monoclonal

KW - Base Sequence

KW - CHO Cells

KW - Cricetinae

KW - DNA Primers

KW - Growth Inhibitors

KW - Humans

KW - Immunization

KW - Interleukin-6

KW - Leukemia Inhibitory Factor

KW - Leukemia Inhibitory Factor Receptor alpha Subunit

KW - Lymphokines

KW - Mice

KW - Mice, Inbred BALB C

KW - Polymerase Chain Reaction

KW - Receptors, Cytokine

KW - Receptors, OSM-LIF

KW - Recombinant Fusion Proteins

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U2 - 10.1016/S0022-1759(97)00074-4

DO - 10.1016/S0022-1759(97)00074-4

M3 - Article

VL - 205

SP - 177

EP - 190

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 2

ER -