Real-time laser speckle contrast imaging for intraoperative neurovascular blood flow assessment: animal experimental study

Anton N Konovalov; Fyodor V Grebenev; Dmitry Stavtsev; Igor O. Kozlov; Gadjiagaev Vadim; Gennadii A. Piavchenko; Dmitry V. Telyshev; Alexander Yu.  Gerasimenko; Igor Meglinski; Savely  Zalogin; Anton  Artemyev; Grigorii  Golodnev; Tatiana  Shumeiko; Shalva  Eliava

doi:10.1038/s41598-023-51022-2

Real-time laser speckle contrast imaging for intraoperative neurovascular blood flow assessment: animal experimental study

Anton N Konovalov^*, Fyodor V Grebenev, Dmitry Stavtsev, Igor O. Kozlov, Gadjiagaev Vadim, Gennadii A. Piavchenko, Dmitry V. Telyshev, Alexander Yu. Gerasimenko, Igor Meglinski^*, Savely Zalogin, Anton Artemyev, Grigorii Golodnev, Tatiana Shumeiko, Shalva Eliava

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The use of various blood flow control methods in neurovascular interventions is crucial for reducing postoperative complications. Neurosurgeons worldwide use different methods, such as contact Dopplerography, intraoperative indocyanine videoangiography (ICG) video angiography, fluorescein angiography, flowmetry, intraoperative angiography, and direct angiography. However, there is no noninvasive method that can assess the presence of blood flow in the vessels of the brain without the introduction of fluorescent substances throughout the intervention. The real-time laser-speckle contrast imaging (LSCI) method was studied for its effectiveness in controlling blood flow in standard cerebrovascular surgery cases in rat common carotid arteries, such as proximal occlusion, trapping, reperfusion, anastomosis, and intraoperative vessel thrombosis. The real-time LSCI method is a promising method for use in neurosurgical practice. This approach allows timely diagnosis of intraoperative disturbance of blood flow in vessels in cases of clip occlusion or thrombosis. Additionally, LSCI allows us to reliably confirm the functioning of the anastomosis and reperfusion after removal of the clips and thrombolysis in real time. An unresolved limitation of the method is noise from movements, but this does not reduce the value of the method. Additional research is required to improve the quality of the data obtained.

Original language	English
Article number	1735
Journal	Scientific Reports
Volume	14
Issue number	1
Early online date	19 Jan 2024
DOIs	https://doi.org/10.1038/s41598-023-51022-2
Publication status	E-pub ahead of print - 19 Jan 2024

Bibliographical note

Copyright © 2024, The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.

Data availability: The datasets generated and/or analysed during the current study are available in the repository, https://1drv.
ms/f/s!AmNlRMtkMjGrgtZv-54XcCQCcq55MQ?e=cBlHa2

Keywords

Animals
Coloring Agents
Fluorescein Angiography
Indocyanine Green
Laser Speckle Contrast Imaging
Rats
Thrombosis

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10.1038/s41598-023-51022-2Licence: CC BY 4.0

s41598-023-51022-2
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Final published version, 3.6 MBLicence: CC BY 4.0

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Konovalov, A. N., Grebenev, F. V., Stavtsev, D., Kozlov, I. O., Vadim, G., Piavchenko, G. A., Telyshev, D. V., Gerasimenko, A. Y., Meglinski, I., Zalogin, S., Artemyev, A., Golodnev, G., Shumeiko, T., & Eliava, S. (2024). Real-time laser speckle contrast imaging for intraoperative neurovascular blood flow assessment: animal experimental study. Scientific Reports, 14(1), Article 1735. Advance online publication. https://doi.org/10.1038/s41598-023-51022-2

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abstract = "The use of various blood flow control methods in neurovascular interventions is crucial for reducing postoperative complications. Neurosurgeons worldwide use different methods, such as contact Dopplerography, intraoperative indocyanine videoangiography (ICG) video angiography, fluorescein angiography, flowmetry, intraoperative angiography, and direct angiography. However, there is no noninvasive method that can assess the presence of blood flow in the vessels of the brain without the introduction of fluorescent substances throughout the intervention. The real-time laser-speckle contrast imaging (LSCI) method was studied for its effectiveness in controlling blood flow in standard cerebrovascular surgery cases in rat common carotid arteries, such as proximal occlusion, trapping, reperfusion, anastomosis, and intraoperative vessel thrombosis. The real-time LSCI method is a promising method for use in neurosurgical practice. This approach allows timely diagnosis of intraoperative disturbance of blood flow in vessels in cases of clip occlusion or thrombosis. Additionally, LSCI allows us to reliably confirm the functioning of the anastomosis and reperfusion after removal of the clips and thrombolysis in real time. An unresolved limitation of the method is noise from movements, but this does not reduce the value of the method. Additional research is required to improve the quality of the data obtained.",

keywords = "Animals, Coloring Agents, Fluorescein Angiography, Indocyanine Green, Laser Speckle Contrast Imaging, Rats, Thrombosis",

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note = "Copyright {\textcopyright} 2024, The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/. Data availability: The datasets generated and/or analysed during the current study are available in the repository, https://1drv. ms/f/s!AmNlRMtkMjGrgtZv-54XcCQCcq55MQ?e=cBlHa2",

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Konovalov, AN, Grebenev, FV, Stavtsev, D, Kozlov, IO, Vadim, G, Piavchenko, GA, Telyshev, DV, Gerasimenko, AY, Meglinski, I, Zalogin, S, Artemyev, A, Golodnev, G, Shumeiko, T & Eliava, S 2024, 'Real-time laser speckle contrast imaging for intraoperative neurovascular blood flow assessment: animal experimental study', Scientific Reports, vol. 14, no. 1, 1735. https://doi.org/10.1038/s41598-023-51022-2

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AU - Konovalov, Anton N

AU - Grebenev, Fyodor V

AU - Stavtsev, Dmitry

AU - Kozlov, Igor O.

AU - Vadim, Gadjiagaev

AU - Piavchenko, Gennadii A.

AU - Telyshev, Dmitry V.

AU - Gerasimenko, Alexander Yu.

AU - Meglinski, Igor

AU - Zalogin, Savely

AU - Artemyev, Anton

AU - Golodnev, Grigorii

AU - Shumeiko, Tatiana

AU - Eliava, Shalva

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Real-time laser speckle contrast imaging for intraoperative neurovascular blood flow assessment: animal experimental study

Abstract

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Keywords

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