Abstract
Original language | English |
---|---|
Pages (from-to) | 1242-1250 |
Number of pages | 9 |
Journal | Mini - Reviews in Medicinal Chemistry |
Volume | 9 |
Issue number | 10 |
Publication status | Published - Sep 2009 |
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Keywords
- animals
- disulfides
- drug carriers
- gene transfer techniques
- humans
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Reducible disulfide-based non-viral gene delivery systems. / Ouyang, Defang; Shah, Neha; Zhang, Hong; Smith, Sean C.; Parekh, Harendra S.
In: Mini - Reviews in Medicinal Chemistry, Vol. 9, No. 10, 09.2009, p. 1242-1250.Research output: Contribution to journal › Article
TY - JOUR
T1 - Reducible disulfide-based non-viral gene delivery systems
AU - Ouyang, Defang
AU - Shah, Neha
AU - Zhang, Hong
AU - Smith, Sean C.
AU - Parekh, Harendra S.
PY - 2009/9
Y1 - 2009/9
N2 - The design and synthesis of safe efficient non-viral vectors for gene delivery has attracted significant attention in recent years due primarily to the severe side-effect profile reported with the use of their viral counterparts. Previous experiments have revealed that the strong interaction between the carriers and nucleic acid may well hinder the release of the gene from the complex in the cytosol adversely affecting transfection efficiency. However, incorporating reducible disulfide bonds within the delivery systems themselves which are then cleaved in the glutathione-rich intracellular environment may help in solving this puzzle. This review focuses on recent development of these reducible carriers. The biological rationale and approaches to the synthesis of reducible vectors are discussed in detail. The in vitro and in vivo evaluations of reducible carriers are also summarized and it is evident that they offer a promising approach in non-viral gene delivery system design.
AB - The design and synthesis of safe efficient non-viral vectors for gene delivery has attracted significant attention in recent years due primarily to the severe side-effect profile reported with the use of their viral counterparts. Previous experiments have revealed that the strong interaction between the carriers and nucleic acid may well hinder the release of the gene from the complex in the cytosol adversely affecting transfection efficiency. However, incorporating reducible disulfide bonds within the delivery systems themselves which are then cleaved in the glutathione-rich intracellular environment may help in solving this puzzle. This review focuses on recent development of these reducible carriers. The biological rationale and approaches to the synthesis of reducible vectors are discussed in detail. The in vitro and in vivo evaluations of reducible carriers are also summarized and it is evident that they offer a promising approach in non-viral gene delivery system design.
KW - animals
KW - disulfides
KW - drug carriers
KW - gene transfer techniques
KW - humans
UR - http://www.scopus.com/inward/record.url?scp=70450215557&partnerID=8YFLogxK
UR - http://www.benthamscience.com/contents-JCode-MRMC-Vol-00000009-Iss-00000010.htm
M3 - Article
C2 - 19817714
VL - 9
SP - 1242
EP - 1250
JO - Mini - Reviews in Medicinal Chemistry
JF - Mini - Reviews in Medicinal Chemistry
SN - 1389-5575
IS - 10
ER -