Reduction of DILP2 in Drosophila triages a metabolic phenotype from lifespan revealing redundancy and compensation among DILPs

Susan Broughton, Nazif Alic, Cathy Slack, Timothy Bass, Tomoatsu Ikeya, Giovanna Vinti, Anna Maria Tommasi, Yasmine Driege, Ernst Hafen, Linda Partridge

Research output: Contribution to journalArticle

Abstract

The insulin/IGF-like signalling (IIS) pathway has diverse functions in all multicellular organisms, including determination of lifespan. The seven insulin-like peptides (DILPs) in Drosophila are expressed in a stage- and tissue-specific manner. Partial ablation of the median neurosecretory cells (mNSCs) in the brain, which produce three DILPs, extends lifespan, reduces fecundity, alters lipid and carbohydrate metabolism and increases oxidative stress resistance. To determine if reduced expression of DILPs is causal in these effects, and to investigate possible functional diversification and redundancy between DILPs, we used RNA interference to lower specifically the transcript and protein levels of dilp2, the most highly expressed of the mNSC-derived DILPs. We found that DILP2 was limiting only for the increased whole-body trehalose content associated with mNSC-ablation. We observed a compensatory increase in dilp3 and 5 mRNA upon dilp2 knock down. By manipulation of dfoxo and dlnR, we showed that the increase in dilp3 is regulated via autocrine insulin signaling in the mNSCs. Our study demonstrates that, despite the correlation between reduced dilp2 mRNA levels and lifespan-extension often observed, DILP2 reduction is not sufficient to extend lifespan. Nor is the increased trehalose storage associated with reduced IIS sufficient to extend lifespan. To understand the normal regulation of expression of the dilps and any functional diversification between them will require independent control of the expression of different dilps.

LanguageEnglish
Article numbere3721
JournalPLoS ONE
Volume3
Issue number11
DOIs
Publication statusPublished - 13 Nov 2008

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Triage
neurosecretory cells
Drosophila
Redundancy
Insulin
Phenotype
phenotype
insulin
Trehalose
Ablation
trehalose
Autocrine Communication
Messenger RNA
Oxidative stress
Carbohydrate Metabolism
RNA Interference
Lipid Metabolism
Fertility
carbohydrate metabolism
Brain

Bibliographical note

© 2008 Broughton et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Cite this

Broughton, Susan ; Alic, Nazif ; Slack, Cathy ; Bass, Timothy ; Ikeya, Tomoatsu ; Vinti, Giovanna ; Tommasi, Anna Maria ; Driege, Yasmine ; Hafen, Ernst ; Partridge, Linda. / Reduction of DILP2 in Drosophila triages a metabolic phenotype from lifespan revealing redundancy and compensation among DILPs. In: PLoS ONE. 2008 ; Vol. 3, No. 11.
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Broughton, S, Alic, N, Slack, C, Bass, T, Ikeya, T, Vinti, G, Tommasi, AM, Driege, Y, Hafen, E & Partridge, L 2008, 'Reduction of DILP2 in Drosophila triages a metabolic phenotype from lifespan revealing redundancy and compensation among DILPs' PLoS ONE, vol. 3, no. 11, e3721. https://doi.org/10.1371/journal.pone.0003721

Reduction of DILP2 in Drosophila triages a metabolic phenotype from lifespan revealing redundancy and compensation among DILPs. / Broughton, Susan; Alic, Nazif; Slack, Cathy; Bass, Timothy; Ikeya, Tomoatsu; Vinti, Giovanna; Tommasi, Anna Maria; Driege, Yasmine; Hafen, Ernst; Partridge, Linda.

In: PLoS ONE, Vol. 3, No. 11, e3721, 13.11.2008.

Research output: Contribution to journalArticle

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AU - Driege, Yasmine

AU - Hafen, Ernst

AU - Partridge, Linda

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