MRI of fluids containing lipid coated microbubbles has been shown to be an effective toot for measuring the local fluid pressure. However, the intrinsically buoyant nature of these microbubbles precludes lengthy measurements due to their vertical migration under gravity and pressure-induced coalescence. A novel preparation is presented which is shown to minimize both these effects for at least 25 min. By using a 2% polysaccharide gel base with a small concentration of glycerol and 1,2-distearoyl-sn-glycero-3-phosphocholine coated gas microbubbles, MR measurements are made for pressures between 0.95 and 1.44 bar. The signal drifts due to migration and amalgamation are shown to be minimized for such an experiment whilst yielding very high NMR sensitivities up to 38% signal change per bar.
- gas filled lipid coated microbubbles
- contrast agent
Morris, R. H., Bencsik, M., Nestle, N., Galvosas, P., Fairhurst, D., Vangala, A., Perrie, Y., & McHale, G. (2008). Robust spatially resolved pressure measurements using MRI with novel buoyant advection-free preparations of stable microbubbles in polysaccharide gels. Journal of Magnetic Resonance, 193(2), 159-167. https://doi.org/10.1016/j.jmr.2008.04.025