Titanium phosphate glass microspheres for bone tissue engineering

Nilay J. Lakhkar, Jeong-Hui Park, Nicola J. Mordan, Vehid Salih, Ivan B. Wall, Hae-Won Kim, Scott P. King, John V. Hanna, Richard A. Martin, Owen Addison, J.F.W. Mosselmans, Jonathan C. Knowles

Research output: Contribution to journalArticlepeer-review


We have demonstrated the successful production of titanium phosphate glass microspheres in the size range of ~10-200 µm using an inexpensive, efficient, easily scalable process and assessed their use in bone tissue engineering applications. Glasses of the following compositions were prepared by melt-quench techniques: 0.5P2O5-0.4CaO-(0.1 - x)Na2O-xTiO2, where x = 0.03, 0.05 and 0.07 mol fraction (denoted as Ti3, Ti5 and Ti7 respectively). Several characterization studies such as differential thermal analysis, degradation (performed using a novel time lapse imaging technique) and pH and ion release measurements revealed significant densification of the glass structure with increased incorporation of TiO2 in the glass from 3 to 5 mol.%, although further TiO2 incorporation up to 7 mol.% did not affect the glass structure to the same extent. Cell culture studies performed using MG63 cells over a 7-day period clearly showed the ability of the microspheres to provide a stable surface for cell attachment, growth and proliferation. Taken together, the results confirm that 5 mol.% TiO2 glass microspheres, on account of their relative ease of preparation and favourable biocompatibility, are worthy candidates for use as substrate materials in bone tissue engineering applications.

Original languageEnglish
Pages (from-to)4181-4190
Number of pages10
JournalActa Biomaterialia
Issue number11
Early online date23 Jul 2012
Publication statusPublished - Nov 2012

Bibliographical note

NOTICE: this is the author’s version of a work that was accepted for publication in Acta biomaterialia. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Lakhkar, N. J., Park, J-H., Mordan, N. J., Salih, V., Wall, I. B., Kim, H-W., King, S. P., Hanna, J. V. , Martin, R. A., Addison, O., Mosselmans, J. F. W., & Knowles, J. C. (2012). Titanium phosphate glass microspheres for bone tissue engineering. Acta biomaterialia, vol. 8, No. 11 (2012) DOI http://dx.doi.org/10.1016/j.actbio.2012.07.023


  • titanium
  • phosphate glass
  • microspheres
  • bone cells
  • tissue engineering


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