Transcranial Photosensitizer-Free Laser Treatment of Glioblastoma in Rat Brain

Oxana Semyachkina-Glushkovskaya; Sergey Sokolovski; Ivan Fedosov; Alexander Shirokov; Nikita Navolokin; Alla Bucharskaya; Inna Blokhina; Andrey Terskov; Alexander Dubrovski; Valeria Telnova; Anna Tzven; Maria Tzoy; Arina Evsukova; Daria Zhlatogosrkaya; Viktoria Adushkina; Alexander Dmitrenko; Maria Manzhaeva; Valeria Krupnova; Alessio Noghero; Denis Bragin; Olga Bragina; Ekaterina Borisova; Jürgen Kurths; Edik Rafailov

doi:10.3390/ijms241813696

Transcranial Photosensitizer-Free Laser Treatment of Glioblastoma in Rat Brain

Oxana Semyachkina-Glushkovskaya, Sergey Sokolovski, Ivan Fedosov, Alexander Shirokov, Nikita Navolokin, Alla Bucharskaya, Inna Blokhina, Andrey Terskov, Alexander Dubrovski, Valeria Telnova, Anna Tzven, Maria Tzoy, Arina Evsukova, Daria Zhlatogosrkaya, Viktoria Adushkina, Alexander Dmitrenko, Maria Manzhaeva, Valeria Krupnova, Alessio Noghero, Denis BraginOlga Bragina, Ekaterina Borisova, Jürgen Kurths, Edik Rafailov

Aston Institute of Photonic Technologies (AiPT)

Research output: Contribution to journal › Article › peer-review

Abstract

Over sixty years, laser technologies have undergone a technological revolution and become one of the main tools in biomedicine, particularly in neuroscience, neurodegenerative diseases and brain tumors. Glioblastoma is the most lethal form of brain cancer, with very limited treatment options and a poor prognosis. In this study on rats, we demonstrate that glioblastoma (GBM) growth can be suppressed by photosensitizer-free laser treatment (PS-free-LT) using a quantum-dot-based 1267 nm laser diode. This wavelength, highly absorbed by oxygen, is capable of turning triplet oxygen to singlet form. Applying 1267 nm laser irradiation for a 4 week course with a total dose of 12.7 kJ/cm2 firmly suppresses GBM growth and increases survival rate from 34% to 64%, presumably via LT-activated apoptosis, inhibition of the proliferation of tumor cells, a reduction in intracranial pressure and stimulation of the lymphatic drainage and clearing functions. PS-free-LT is a promising breakthrough technology in non- or minimally invasive therapy for superficial GBMs in infants as well as in adult patients with high photosensitivity or an allergic reaction to PSs.

Original language	English
Article number	13696
Number of pages	25
Journal	International Journal of Molecular Sciences
Volume	24
Issue number	18
Early online date	5 Sept 2023
DOIs	https://doi.org/10.3390/ijms241813696
Publication status	Published - 5 Sept 2023

Bibliographical note

Copyright © 2023 by the authors. Licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and
conditions of the Creative CommonsAttribution (CC BY) license (https://
creativecommons.org/licenses/by/4.0/).

Funding: O.S.-G., I.F., A.S., N.N., I.B., A.T., A.D., M.Z., A.E., D.Z., V.A., A.D., M.M. and V.K. were supported by a grant (No. 23-75-30001) from the Russian Science Foundation; N.N., D.Z. and V.A. were supported by a grant (No. 21-75-10088) from the Russian Science Foundation; O.S.-G., I.F., A.S., N.N., I.B., A.T. and M.T. were supported by a grant from the Russian Ministry of Science and Higher
Education (No. 075-15-2022-1094); D.B. was supported by NIH R21NS091600 and R01 NS112808. E.R. and S.S. gratefully acknowledge funding from the HE Pathfinder GlioLight project (number 101129705). All cytological experiments, microscopy in the light field and fluorescence modes and electron microscopic examinations were carried out using equipment from the research equipment
center “SYMBIOSIS” within research project No. GR 121031100266-3 (IBPPM RAS).

Keywords

glioblastoma
phototherapy
quantum-dot 1267 nm laser
NIR laser oxidative cell damaging
lymphatic system

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10.3390/ijms241813696Licence: CC BY 4.0

O Semyachkina-Glushkovskaya et al_Transcranial Photosensitizer-Free Laser Treatment Glioblastoma Rat
Copyright © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative CommonsAttribution (CC BY) license (https:// creativecommons.org/licenses/by/4.0/)
Final published version, 11.4 MBLicence: CC BY 4.0

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Semyachkina-Glushkovskaya, O., Sokolovski, S., Fedosov, I., Shirokov, A., Navolokin, N., Bucharskaya, A., Blokhina, I., Terskov, A., Dubrovski, A., Telnova, V., Tzven, A., Tzoy, M., Evsukova, A., Zhlatogosrkaya, D., Adushkina, V., Dmitrenko, A., Manzhaeva, M., Krupnova, V., Noghero, A., ... Rafailov, E. (2023). Transcranial Photosensitizer-Free Laser Treatment of Glioblastoma in Rat Brain. International Journal of Molecular Sciences, 24(18), Article 13696. https://doi.org/10.3390/ijms241813696

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abstract = "Over sixty years, laser technologies have undergone a technological revolution and become one of the main tools in biomedicine, particularly in neuroscience, neurodegenerative diseases and brain tumors. Glioblastoma is the most lethal form of brain cancer, with very limited treatment options and a poor prognosis. In this study on rats, we demonstrate that glioblastoma (GBM) growth can be suppressed by photosensitizer-free laser treatment (PS-free-LT) using a quantum-dot-based 1267 nm laser diode. This wavelength, highly absorbed by oxygen, is capable of turning triplet oxygen to singlet form. Applying 1267 nm laser irradiation for a 4 week course with a total dose of 12.7 kJ/cm2 firmly suppresses GBM growth and increases survival rate from 34% to 64%, presumably via LT-activated apoptosis, inhibition of the proliferation of tumor cells, a reduction in intracranial pressure and stimulation of the lymphatic drainage and clearing functions. PS-free-LT is a promising breakthrough technology in non- or minimally invasive therapy for superficial GBMs in infants as well as in adult patients with high photosensitivity or an allergic reaction to PSs.",

keywords = "glioblastoma, phototherapy, quantum-dot 1267 nm laser, NIR laser oxidative cell damaging, lymphatic system",

author = "Oxana Semyachkina-Glushkovskaya and Sergey Sokolovski and Ivan Fedosov and Alexander Shirokov and Nikita Navolokin and Alla Bucharskaya and Inna Blokhina and Andrey Terskov and Alexander Dubrovski and Valeria Telnova and Anna Tzven and Maria Tzoy and Arina Evsukova and Daria Zhlatogosrkaya and Viktoria Adushkina and Alexander Dmitrenko and Maria Manzhaeva and Valeria Krupnova and Alessio Noghero and Denis Bragin and Olga Bragina and Ekaterina Borisova and J{\"u}rgen Kurths and Edik Rafailov",

note = "Copyright {\textcopyright} 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative CommonsAttribution (CC BY) license (https:// creativecommons.org/licenses/by/4.0/). Funding: O.S.-G., I.F., A.S., N.N., I.B., A.T., A.D., M.Z., A.E., D.Z., V.A., A.D., M.M. and V.K. were supported by a grant (No. 23-75-30001) from the Russian Science Foundation; N.N., D.Z. and V.A. were supported by a grant (No. 21-75-10088) from the Russian Science Foundation; O.S.-G., I.F., A.S., N.N., I.B., A.T. and M.T. were supported by a grant from the Russian Ministry of Science and Higher Education (No. 075-15-2022-1094); D.B. was supported by NIH R21NS091600 and R01 NS112808. E.R. and S.S. gratefully acknowledge funding from the HE Pathfinder GlioLight project (number 101129705). All cytological experiments, microscopy in the light field and fluorescence modes and electron microscopic examinations were carried out using equipment from the research equipment center “SYMBIOSIS” within research project No. GR 121031100266-3 (IBPPM RAS).",

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Semyachkina-Glushkovskaya, O, Sokolovski, S, Fedosov, I, Shirokov, A, Navolokin, N, Bucharskaya, A, Blokhina, I, Terskov, A, Dubrovski, A, Telnova, V, Tzven, A, Tzoy, M, Evsukova, A, Zhlatogosrkaya, D, Adushkina, V, Dmitrenko, A, Manzhaeva, M, Krupnova, V, Noghero, A, Bragin, D, Bragina, O, Borisova, E, Kurths, J & Rafailov, E 2023, 'Transcranial Photosensitizer-Free Laser Treatment of Glioblastoma in Rat Brain', International Journal of Molecular Sciences, vol. 24, no. 18, 13696. https://doi.org/10.3390/ijms241813696

TY - JOUR

T1 - Transcranial Photosensitizer-Free Laser Treatment of Glioblastoma in Rat Brain

AU - Semyachkina-Glushkovskaya, Oxana

AU - Sokolovski, Sergey

AU - Fedosov, Ivan

AU - Shirokov, Alexander

AU - Navolokin, Nikita

AU - Bucharskaya, Alla

AU - Blokhina, Inna

AU - Terskov, Andrey

AU - Dubrovski, Alexander

AU - Telnova, Valeria

AU - Tzven, Anna

AU - Tzoy, Maria

AU - Evsukova, Arina

AU - Zhlatogosrkaya, Daria

AU - Adushkina, Viktoria

AU - Dmitrenko, Alexander

AU - Manzhaeva, Maria

AU - Krupnova, Valeria

AU - Noghero, Alessio

AU - Bragin, Denis

AU - Bragina, Olga

AU - Borisova, Ekaterina

AU - Kurths, Jürgen

AU - Rafailov, Edik

N1 - Copyright © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative CommonsAttribution (CC BY) license (https:// creativecommons.org/licenses/by/4.0/). Funding: O.S.-G., I.F., A.S., N.N., I.B., A.T., A.D., M.Z., A.E., D.Z., V.A., A.D., M.M. and V.K. were supported by a grant (No. 23-75-30001) from the Russian Science Foundation; N.N., D.Z. and V.A. were supported by a grant (No. 21-75-10088) from the Russian Science Foundation; O.S.-G., I.F., A.S., N.N., I.B., A.T. and M.T. were supported by a grant from the Russian Ministry of Science and Higher Education (No. 075-15-2022-1094); D.B. was supported by NIH R21NS091600 and R01 NS112808. E.R. and S.S. gratefully acknowledge funding from the HE Pathfinder GlioLight project (number 101129705). All cytological experiments, microscopy in the light field and fluorescence modes and electron microscopic examinations were carried out using equipment from the research equipment center “SYMBIOSIS” within research project No. GR 121031100266-3 (IBPPM RAS).

PY - 2023/9/5

Y1 - 2023/9/5

N2 - Over sixty years, laser technologies have undergone a technological revolution and become one of the main tools in biomedicine, particularly in neuroscience, neurodegenerative diseases and brain tumors. Glioblastoma is the most lethal form of brain cancer, with very limited treatment options and a poor prognosis. In this study on rats, we demonstrate that glioblastoma (GBM) growth can be suppressed by photosensitizer-free laser treatment (PS-free-LT) using a quantum-dot-based 1267 nm laser diode. This wavelength, highly absorbed by oxygen, is capable of turning triplet oxygen to singlet form. Applying 1267 nm laser irradiation for a 4 week course with a total dose of 12.7 kJ/cm2 firmly suppresses GBM growth and increases survival rate from 34% to 64%, presumably via LT-activated apoptosis, inhibition of the proliferation of tumor cells, a reduction in intracranial pressure and stimulation of the lymphatic drainage and clearing functions. PS-free-LT is a promising breakthrough technology in non- or minimally invasive therapy for superficial GBMs in infants as well as in adult patients with high photosensitivity or an allergic reaction to PSs.

AB - Over sixty years, laser technologies have undergone a technological revolution and become one of the main tools in biomedicine, particularly in neuroscience, neurodegenerative diseases and brain tumors. Glioblastoma is the most lethal form of brain cancer, with very limited treatment options and a poor prognosis. In this study on rats, we demonstrate that glioblastoma (GBM) growth can be suppressed by photosensitizer-free laser treatment (PS-free-LT) using a quantum-dot-based 1267 nm laser diode. This wavelength, highly absorbed by oxygen, is capable of turning triplet oxygen to singlet form. Applying 1267 nm laser irradiation for a 4 week course with a total dose of 12.7 kJ/cm2 firmly suppresses GBM growth and increases survival rate from 34% to 64%, presumably via LT-activated apoptosis, inhibition of the proliferation of tumor cells, a reduction in intracranial pressure and stimulation of the lymphatic drainage and clearing functions. PS-free-LT is a promising breakthrough technology in non- or minimally invasive therapy for superficial GBMs in infants as well as in adult patients with high photosensitivity or an allergic reaction to PSs.

KW - glioblastoma

KW - phototherapy

KW - quantum-dot 1267 nm laser

KW - NIR laser oxidative cell damaging

KW - lymphatic system

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DO - 10.3390/ijms241813696

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JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 18

M1 - 13696

ER -

Transcranial Photosensitizer-Free Laser Treatment of Glioblastoma in Rat Brain

Abstract

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Keywords

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