Verification of NADH content measurements by portable optical diagnostic system in living brain tissue

Evgeny Zherebtsov, Plamena Angelova, Sergei G. Sokolovski, Andrey Abramov, Edik U. Rafailov, Jürgen Popp (Editor), Valery V. Tuchin, Francesco S. Pavone (Editor)

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The overall aim of this study was verification of the possibility to register the change of NADH fluorescence in live tissue by a portable diagnostical laser system with fibre optical probe output and excitation by compact semiconductor UV light source. The measurements were conducted in fresh brain tissue slices of Wistar rat pups. The fluorescence measurements were conducted simultaneously at intervals of 5 s by the microscopic system with excitation at 360 nm and registering of the emitted fluorescence light at 455 nm and by the tested diagnostical system equipped with the fibre optical probe with excitation at 365 nm and registration of the fluorescence spectrum by the inbuilt spectroscopic subsystem. To modulate the mitochondrial function in the living cells, in the chamber sequentially were added 1 μM FCCP and 1 mM NaCN. The comparisons between the curves registered by the methods allowed us to find well agreement between the microscopic measurements and measurements by the fibre optical probe. The obtained results prove that the tested diagnostic system is capable of sensing the changes in brain metabolic activity associated with the NADH content alterations within the physiological range.
Original languageEnglish
Title of host publicationProceedings of SPIE
Subtitle of host publicationBiophotonics: Photonic Solutions for Better Health Care VI
PublisherSPIE
Number of pages5
Volume10685
DOIs
Publication statusPublished - 17 May 2018
EventBiophotonics: Photonic Solutions for Better Health Care - Strasbourg, France
Duration: 22 Apr 201826 Apr 2018

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume10685
ISSN (Print)0277-786X

Conference

ConferenceBiophotonics: Photonic Solutions for Better Health Care
Period22/04/1826/04/18

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brain
fluorescence
optical fibers
probes
excitation
registers
rats
light sources
chambers
intervals
output
curves
lasers

Bibliographical note

Copyright 2018 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Cite this

Zherebtsov, E., Angelova, P., Sokolovski, S. G., Abramov, A., Rafailov, E. U., Popp, J. (Ed.), ... Pavone, F. S. (Ed.) (2018). Verification of NADH content measurements by portable optical diagnostic system in living brain tissue. In Proceedings of SPIE : Biophotonics: Photonic Solutions for Better Health Care VI (Vol. 10685 ). [106854E] (Proceedings of SPIE; Vol. 10685 ). SPIE. https://doi.org/10.1117/12.2307552
Zherebtsov, Evgeny ; Angelova, Plamena ; Sokolovski, Sergei G. ; Abramov, Andrey ; Rafailov, Edik U. ; Popp, Jürgen (Editor) ; Tuchin, Valery V. ; Pavone, Francesco S. (Editor). / Verification of NADH content measurements by portable optical diagnostic system in living brain tissue. Proceedings of SPIE : Biophotonics: Photonic Solutions for Better Health Care VI. Vol. 10685 SPIE, 2018. (Proceedings of SPIE).
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Zherebtsov, E, Angelova, P, Sokolovski, SG, Abramov, A, Rafailov, EU, Popp, J (ed.), Tuchin, VV & Pavone, FS (ed.) 2018, Verification of NADH content measurements by portable optical diagnostic system in living brain tissue. in Proceedings of SPIE : Biophotonics: Photonic Solutions for Better Health Care VI. vol. 10685 , 106854E, Proceedings of SPIE, vol. 10685 , SPIE, Biophotonics: Photonic Solutions for Better Health Care, 22/04/18. https://doi.org/10.1117/12.2307552

Verification of NADH content measurements by portable optical diagnostic system in living brain tissue. / Zherebtsov, Evgeny; Angelova, Plamena; Sokolovski, Sergei G.; Abramov, Andrey; Rafailov, Edik U.; Popp, Jürgen (Editor); Tuchin, Valery V.; Pavone, Francesco S. (Editor).

Proceedings of SPIE : Biophotonics: Photonic Solutions for Better Health Care VI. Vol. 10685 SPIE, 2018. 106854E (Proceedings of SPIE; Vol. 10685 ).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - The overall aim of this study was verification of the possibility to register the change of NADH fluorescence in live tissue by a portable diagnostical laser system with fibre optical probe output and excitation by compact semiconductor UV light source. The measurements were conducted in fresh brain tissue slices of Wistar rat pups. The fluorescence measurements were conducted simultaneously at intervals of 5 s by the microscopic system with excitation at 360 nm and registering of the emitted fluorescence light at 455 nm and by the tested diagnostical system equipped with the fibre optical probe with excitation at 365 nm and registration of the fluorescence spectrum by the inbuilt spectroscopic subsystem. To modulate the mitochondrial function in the living cells, in the chamber sequentially were added 1 μM FCCP and 1 mM NaCN. The comparisons between the curves registered by the methods allowed us to find well agreement between the microscopic measurements and measurements by the fibre optical probe. The obtained results prove that the tested diagnostic system is capable of sensing the changes in brain metabolic activity associated with the NADH content alterations within the physiological range.

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M3 - Conference contribution

VL - 10685

T3 - Proceedings of SPIE

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Zherebtsov E, Angelova P, Sokolovski SG, Abramov A, Rafailov EU, Popp J, (ed.) et al. Verification of NADH content measurements by portable optical diagnostic system in living brain tissue. In Proceedings of SPIE : Biophotonics: Photonic Solutions for Better Health Care VI. Vol. 10685 . SPIE. 2018. 106854E. (Proceedings of SPIE). https://doi.org/10.1117/12.2307552