An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples

Nicolle Breusing, Tilman Grune, Luka Andrisic, Mustafa Atalay, Grzegorz Bartosz, Fiorella Biasi, Suzana Borovic, Laura Bravo, Isidre Casals, Rosario Casillas, Anca Dinischiotu, Joanna Drzewinska, Heidemarie Faber, Norsyahida M. Fauzi, Agnieszka Gajewska, Juan Gambini, Daniela Gradinaru, Tarja Kokkola, Antonin Lojek, Wojciech ŁuczajDenisa Margina, Cinzia Mascia, Raquel Mateos, Andreas Meinitzer, María T. Mitjavila, Lidija Mrakovcic, Maria C. Munteanu, Martina Podborska, Giuseppe Poli, Paulina Sicinska, Elzbieta Skrzydlewska, Jose Vina, Ingrid Wiswedel, Neven Zarkovic, Sieglinde Zelzer, Corinne M Spickett

Research output: Contribution to journalArticlepeer-review


Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F(2)-isoprostanes are widely used as markers of oxidative stress in vitro and in vivo. This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coefficients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity.
Original languageEnglish
Pages (from-to)1203-1215
Number of pages13
JournalFree Radical Research
Issue number10
Publication statusPublished - Oct 2010


  • aldehydes
  • high pressure liquid chromatography
  • liquid chromatography
  • clinical chemistry tTests
  • enzyme-linked immunosorbent assay
  • humans
  • isoprostanes
  • lipid peroxidation
  • malondialdehyde
  • mass spectrometry
  • plasma
  • reproducibility of results
  • sensitivity and specificity
  • ultraviolet rays
  • oxidative stress
  • 4-hydroxynonenal
  • F2-isoprostanes


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