An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples

Nicolle Breusing, Tilman Grune, Luka Andrisic, Mustafa Atalay, Grzegorz Bartosz, Fiorella Biasi, Suzana Borovic, Laura Bravo, Isidre Casals, Rosario Casillas, Anca Dinischiotu, Joanna Drzewinska, Heidemarie Faber, Norsyahida M. Fauzi, Agnieszka Gajewska, Juan Gambini, Daniela Gradinaru, Tarja Kokkola, Antonin Lojek, Wojciech Łuczaj & 16 others Denisa Margina, Cinzia Mascia, Raquel Mateos, Andreas Meinitzer, María T. Mitjavila, Lidija Mrakovcic, Maria C. Munteanu, Martina Podborska, Giuseppe Poli, Paulina Sicinska, Elzbieta Skrzydlewska, Jose Vina, Ingrid Wiswedel, Neven Zarkovic, Sieglinde Zelzer, Corinne M Spickett

Research output: Contribution to journalArticle

Abstract

Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F(2)-isoprostanes are widely used as markers of oxidative stress in vitro and in vivo. This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coefficients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity.
LanguageEnglish
Pages1203-1215
Number of pages13
JournalFree Radical Research
Volume44
Issue number10
DOIs
Publication statusPublished - Oct 2010

Fingerprint

Plasma (human)
Lipid Peroxidation
Malondialdehyde
Lipids
Isoprostanes
High Pressure Liquid Chromatography
Plasmas
Oxidative stress
Validation Studies
Edetic Acid
Dosimetry
Oxidative Stress
Irradiation

Keywords

  • aldehydes
  • high pressure liquid chromatography
  • liquid chromatography
  • clinical chemistry tTests
  • enzyme-linked immunosorbent assay
  • humans
  • isoprostanes
  • lipid peroxidation
  • malondialdehyde
  • mass spectrometry
  • plasma
  • reproducibility of results
  • sensitivity and specificity
  • ultraviolet rays
  • oxidative stress
  • 4-hydroxynonenal
  • F2-isoprostanes

Cite this

Breusing, Nicolle ; Grune, Tilman ; Andrisic, Luka ; Atalay, Mustafa ; Bartosz, Grzegorz ; Biasi, Fiorella ; Borovic, Suzana ; Bravo, Laura ; Casals, Isidre ; Casillas, Rosario ; Dinischiotu, Anca ; Drzewinska, Joanna ; Faber, Heidemarie ; Fauzi, Norsyahida M. ; Gajewska, Agnieszka ; Gambini, Juan ; Gradinaru, Daniela ; Kokkola, Tarja ; Lojek, Antonin ; Łuczaj, Wojciech ; Margina, Denisa ; Mascia, Cinzia ; Mateos, Raquel ; Meinitzer, Andreas ; Mitjavila, María T. ; Mrakovcic, Lidija ; Munteanu, Maria C. ; Podborska, Martina ; Poli, Giuseppe ; Sicinska, Paulina ; Skrzydlewska, Elzbieta ; Vina, Jose ; Wiswedel, Ingrid ; Zarkovic, Neven ; Zelzer, Sieglinde ; Spickett, Corinne M. / An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples. In: Free Radical Research. 2010 ; Vol. 44, No. 10. pp. 1203-1215.
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abstract = "Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F(2)-isoprostanes are widely used as markers of oxidative stress in vitro and in vivo. This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coefficients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity.",
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Breusing, N, Grune, T, Andrisic, L, Atalay, M, Bartosz, G, Biasi, F, Borovic, S, Bravo, L, Casals, I, Casillas, R, Dinischiotu, A, Drzewinska, J, Faber, H, Fauzi, NM, Gajewska, A, Gambini, J, Gradinaru, D, Kokkola, T, Lojek, A, Łuczaj, W, Margina, D, Mascia, C, Mateos, R, Meinitzer, A, Mitjavila, MT, Mrakovcic, L, Munteanu, MC, Podborska, M, Poli, G, Sicinska, P, Skrzydlewska, E, Vina, J, Wiswedel, I, Zarkovic, N, Zelzer, S & Spickett, CM 2010, 'An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples' Free Radical Research, vol. 44, no. 10, pp. 1203-1215. https://doi.org/10.3109/10715762.2010.499907

An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples. / Breusing, Nicolle; Grune, Tilman; Andrisic, Luka; Atalay, Mustafa; Bartosz, Grzegorz; Biasi, Fiorella; Borovic, Suzana; Bravo, Laura; Casals, Isidre; Casillas, Rosario; Dinischiotu, Anca; Drzewinska, Joanna; Faber, Heidemarie; Fauzi, Norsyahida M.; Gajewska, Agnieszka; Gambini, Juan; Gradinaru, Daniela; Kokkola, Tarja; Lojek, Antonin; Łuczaj, Wojciech; Margina, Denisa; Mascia, Cinzia; Mateos, Raquel; Meinitzer, Andreas; Mitjavila, María T.; Mrakovcic, Lidija; Munteanu, Maria C.; Podborska, Martina; Poli, Giuseppe; Sicinska, Paulina; Skrzydlewska, Elzbieta; Vina, Jose; Wiswedel, Ingrid; Zarkovic, Neven; Zelzer, Sieglinde; Spickett, Corinne M.

In: Free Radical Research, Vol. 44, No. 10, 10.2010, p. 1203-1215.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples

AU - Breusing, Nicolle

AU - Grune, Tilman

AU - Andrisic, Luka

AU - Atalay, Mustafa

AU - Bartosz, Grzegorz

AU - Biasi, Fiorella

AU - Borovic, Suzana

AU - Bravo, Laura

AU - Casals, Isidre

AU - Casillas, Rosario

AU - Dinischiotu, Anca

AU - Drzewinska, Joanna

AU - Faber, Heidemarie

AU - Fauzi, Norsyahida M.

AU - Gajewska, Agnieszka

AU - Gambini, Juan

AU - Gradinaru, Daniela

AU - Kokkola, Tarja

AU - Lojek, Antonin

AU - Łuczaj, Wojciech

AU - Margina, Denisa

AU - Mascia, Cinzia

AU - Mateos, Raquel

AU - Meinitzer, Andreas

AU - Mitjavila, María T.

AU - Mrakovcic, Lidija

AU - Munteanu, Maria C.

AU - Podborska, Martina

AU - Poli, Giuseppe

AU - Sicinska, Paulina

AU - Skrzydlewska, Elzbieta

AU - Vina, Jose

AU - Wiswedel, Ingrid

AU - Zarkovic, Neven

AU - Zelzer, Sieglinde

AU - Spickett, Corinne M

PY - 2010/10

Y1 - 2010/10

N2 - Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F(2)-isoprostanes are widely used as markers of oxidative stress in vitro and in vivo. This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coefficients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity.

AB - Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F(2)-isoprostanes are widely used as markers of oxidative stress in vitro and in vivo. This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coefficients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity.

KW - aldehydes

KW - high pressure liquid chromatography

KW - liquid chromatography

KW - clinical chemistry tTests

KW - enzyme-linked immunosorbent assay

KW - humans

KW - isoprostanes

KW - lipid peroxidation

KW - malondialdehyde

KW - mass spectrometry

KW - plasma

KW - reproducibility of results

KW - sensitivity and specificity

KW - ultraviolet rays

KW - oxidative stress

KW - 4-hydroxynonenal

KW - F2-isoprostanes

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U2 - 10.3109/10715762.2010.499907

DO - 10.3109/10715762.2010.499907

M3 - Article

VL - 44

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EP - 1215

JO - Free Radical Research

T2 - Free Radical Research

JF - Free Radical Research

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