An ion-sensitive microelectrode study on the effect of a high concentration of ivermectin on chloride balance in the somatic muscle bag cells of Ascaris suum

H R Parri; M B Djamgoz; L Holden-Dye; R J Walker

An ion-sensitive microelectrode study on the effect of a high concentration of ivermectin on chloride balance in the somatic muscle bag cells of Ascaris suum

H R Parri, M B Djamgoz, L Holden-Dye, R J Walker

Research output: Contribution to journal › Article › peer-review

Abstract

Ivermectin has been shown to increase chloride conductances of invertebrate cells. On the muscle cells of the parasitic nematode Ascaris, ivermectin acts as both a GABA receptor antagonist and a chloride channel opener. In this study, ion-sensitive microelectrodes were used to investigate the effect of ivermectin on intracellular Cl- concentration of the somatic muscle bag cells of Ascaris suum. Incubation of muscle cells with ivermectin (10 microM in 1% dimethyl sulphoxide vehicle for 60 min) increased intracellular Cl- by 2.9 mM or 15% compared to controls (P <0.01, n = 6).

Original language	English
Pages (from-to)	421-7
Number of pages	7
Journal	Parasitology
Volume	106
Issue number	4
Publication status	Published - May 1993

Keywords

Animals
Ascaris suum
Chlorides
Dimethyl Sulfoxide
Ions
Ivermectin
Membrane Potentials
Microelectrodes
Muscles
Sensitivity and Specificity
Water-Electrolyte Balance

Cite this

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title = "An ion-sensitive microelectrode study on the effect of a high concentration of ivermectin on chloride balance in the somatic muscle bag cells of Ascaris suum",

abstract = "Ivermectin has been shown to increase chloride conductances of invertebrate cells. On the muscle cells of the parasitic nematode Ascaris, ivermectin acts as both a GABA receptor antagonist and a chloride channel opener. In this study, ion-sensitive microelectrodes were used to investigate the effect of ivermectin on intracellular Cl- concentration of the somatic muscle bag cells of Ascaris suum. Incubation of muscle cells with ivermectin (10 microM in 1% dimethyl sulphoxide vehicle for 60 min) increased intracellular Cl- by 2.9 mM or 15% compared to controls (P <0.01, n = 6).",

keywords = "Animals, Ascaris suum, Chlorides, Dimethyl Sulfoxide, Ions, Ivermectin, Membrane Potentials, Microelectrodes, Muscles, Sensitivity and Specificity, Water-Electrolyte Balance",

author = "Parri, {H R} and Djamgoz, {M B} and L Holden-Dye and Walker, {R J}",

year = "1993",

month = may,

language = "English",

volume = "106 ",

pages = "421--7",

journal = "Parasitology",

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publisher = "Cambridge University Press",

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TY - JOUR

T1 - An ion-sensitive microelectrode study on the effect of a high concentration of ivermectin on chloride balance in the somatic muscle bag cells of Ascaris suum

AU - Parri, H R

AU - Djamgoz, M B

AU - Holden-Dye, L

AU - Walker, R J

PY - 1993/5

Y1 - 1993/5

N2 - Ivermectin has been shown to increase chloride conductances of invertebrate cells. On the muscle cells of the parasitic nematode Ascaris, ivermectin acts as both a GABA receptor antagonist and a chloride channel opener. In this study, ion-sensitive microelectrodes were used to investigate the effect of ivermectin on intracellular Cl- concentration of the somatic muscle bag cells of Ascaris suum. Incubation of muscle cells with ivermectin (10 microM in 1% dimethyl sulphoxide vehicle for 60 min) increased intracellular Cl- by 2.9 mM or 15% compared to controls (P <0.01, n = 6).

AB - Ivermectin has been shown to increase chloride conductances of invertebrate cells. On the muscle cells of the parasitic nematode Ascaris, ivermectin acts as both a GABA receptor antagonist and a chloride channel opener. In this study, ion-sensitive microelectrodes were used to investigate the effect of ivermectin on intracellular Cl- concentration of the somatic muscle bag cells of Ascaris suum. Incubation of muscle cells with ivermectin (10 microM in 1% dimethyl sulphoxide vehicle for 60 min) increased intracellular Cl- by 2.9 mM or 15% compared to controls (P <0.01, n = 6).

KW - Animals

KW - Ascaris suum

KW - Chlorides

KW - Dimethyl Sulfoxide

KW - Ions

KW - Ivermectin

KW - Membrane Potentials

KW - Microelectrodes

KW - Muscles

KW - Sensitivity and Specificity

KW - Water-Electrolyte Balance

UR - https://www.ncbi.nlm.nih.gov/pubmed/8316438

M3 - Article

C2 - 8316438

SN - 0031-1820

VL - 106

SP - 421

EP - 427

JO - Parasitology

JF - Parasitology

IS - 4

ER -

An ion-sensitive microelectrode study on the effect of a high concentration of ivermectin on chloride balance in the somatic muscle bag cells of Ascaris suum

Abstract

Keywords

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