Analysis of clinical isolates of Propionibacterium acnes by optimised RAPD

A.L. Perry; Tony Worthington; Anthony Hilton; Peter A. Lambert; A.J. Stirling; T.S.J. Elliott

doi:10.1016/S0378-1097(03)00720-1

Analysis of clinical isolates of Propionibacterium acnes by optimised RAPD

A.L. Perry, Tony Worthington, Anthony Hilton, Peter A. Lambert^*, A.J. Stirling, T.S.J. Elliott

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Random amplification of polymorphic DNA (RAPD) was evaluated as a genotypic method for typing clinical strains of Propionibacterium acnes. RAPD can suffer from problems of reproducibility if parameters are not standardised. In this study the reaction conditions were optimised by adjusting template DNA concentration and buffer constituents. All isolates were typeable using the optimised RAPD protocol which was found to be highly discriminatory (Simpson's diversity index, 0.98) and reproducible. Typing of P. acnes by optimised RAPD is an invaluable tool for the epidemiological investigation of P. acnes for which no other widely accepted method currently exists. © 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	51-55
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	228
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(03)00720-1
Publication status	Published - 7 Nov 2003

Keywords

molecular typing
propionibacterium acnes
random amplification of polymorphic DNA

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10.1016/S0378-1097(03)00720-1

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title = "Analysis of clinical isolates of Propionibacterium acnes by optimised RAPD",

abstract = "Random amplification of polymorphic DNA (RAPD) was evaluated as a genotypic method for typing clinical strains of Propionibacterium acnes. RAPD can suffer from problems of reproducibility if parameters are not standardised. In this study the reaction conditions were optimised by adjusting template DNA concentration and buffer constituents. All isolates were typeable using the optimised RAPD protocol which was found to be highly discriminatory (Simpson's diversity index, 0.98) and reproducible. Typing of P. acnes by optimised RAPD is an invaluable tool for the epidemiological investigation of P. acnes for which no other widely accepted method currently exists. {\textcopyright} 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.",

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AU - Perry, A.L.

AU - Worthington, Tony

AU - Hilton, Anthony

AU - Lambert, Peter A.

AU - Stirling, A.J.

AU - Elliott, T.S.J.

PY - 2003/11/7

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N2 - Random amplification of polymorphic DNA (RAPD) was evaluated as a genotypic method for typing clinical strains of Propionibacterium acnes. RAPD can suffer from problems of reproducibility if parameters are not standardised. In this study the reaction conditions were optimised by adjusting template DNA concentration and buffer constituents. All isolates were typeable using the optimised RAPD protocol which was found to be highly discriminatory (Simpson's diversity index, 0.98) and reproducible. Typing of P. acnes by optimised RAPD is an invaluable tool for the epidemiological investigation of P. acnes for which no other widely accepted method currently exists. © 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

AB - Random amplification of polymorphic DNA (RAPD) was evaluated as a genotypic method for typing clinical strains of Propionibacterium acnes. RAPD can suffer from problems of reproducibility if parameters are not standardised. In this study the reaction conditions were optimised by adjusting template DNA concentration and buffer constituents. All isolates were typeable using the optimised RAPD protocol which was found to be highly discriminatory (Simpson's diversity index, 0.98) and reproducible. Typing of P. acnes by optimised RAPD is an invaluable tool for the epidemiological investigation of P. acnes for which no other widely accepted method currently exists. © 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

KW - molecular typing

KW - propionibacterium acnes

KW - random amplification of polymorphic DNA

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Analysis of clinical isolates of Propionibacterium acnes by optimised RAPD

Abstract

Keywords

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