TY - JOUR
T1 - Comprehensive two-dimensional liquid chromatography mass spectrometric profiling of the rat hippocampal proteome
AU - Yang, Xiaoping
AU - Levin, Yishai
AU - Rahmoune, Hassan
AU - Ma, Dan
AU - Schöffmann, Stephanie
AU - Umrania, Yagnesh
AU - Guest, Paul C.
AU - Bahn, Sabine
PY - 2011/2
Y1 - 2011/2
N2 - In this study, we performed the first high-throughput and comprehensive proteomic profiling of the rat hippocampal proteome. Using a combination of 2-D LC-MS and data analysis with the Rosetta Elucidator® system, we identified 1340 unique proteins. Functional classification showed that most of these were associated with synaptic function and comprised a high proportion of phosphorylated proteins and analytically challenging classes of membrane proteins such as ion channel receptor subunits.
AB - In this study, we performed the first high-throughput and comprehensive proteomic profiling of the rat hippocampal proteome. Using a combination of 2-D LC-MS and data analysis with the Rosetta Elucidator® system, we identified 1340 unique proteins. Functional classification showed that most of these were associated with synaptic function and comprised a high proportion of phosphorylated proteins and analytically challenging classes of membrane proteins such as ion channel receptor subunits.
KW - 2-D liquid chromatography
KW - Animal proteomics
KW - Hippocampus
KW - Mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=79251519659&partnerID=8YFLogxK
UR - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/pmic.201000525
U2 - 10.1002/pmic.201000525
DO - 10.1002/pmic.201000525
M3 - Article
C2 - 21268280
AN - SCOPUS:79251519659
SN - 1615-9853
VL - 11
SP - 501
EP - 505
JO - Proteomics
JF - Proteomics
IS - 3
ER -