Eukaryotic expression: Developments for structural proteomics

A.R. Aricescu; R. Assenberg; Roslyn M. Bill; D. Busso; V.T. Chang; S.J. Davis; A. Dubrovsky; L. Gustafsson; K. Hedfalk; U. Heinemann; I.M. Jones; D. Ksiazek; C. Lang; K. Maskos; A. Messerschmidt; S. Macieira; Y. Peleg; A. Perrakis; A. Poterszman; G. Schneider; T.K. Sixma; J.L. Sussman; G. Sutton; N. Tarboureich; T. Zeev-Ben-Mordehai; E. Yvonne Jones

doi:10.1107/S0907444906029805

Eukaryotic expression: Developments for structural proteomics

A.R. Aricescu, R. Assenberg, Roslyn M. Bill, D. Busso, V.T. Chang, S.J. Davis, A. Dubrovsky, L. Gustafsson, K. Hedfalk, U. Heinemann, I.M. Jones, D. Ksiazek, C. Lang, K. Maskos, A. Messerschmidt, S. Macieira, Y. Peleg, A. Perrakis, A. Poterszman, G. SchneiderT.K. Sixma, J.L. Sussman, G. Sutton, N. Tarboureich, T. Zeev-Ben-Mordehai, E. Yvonne Jones^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The production of sufficient quantities of protein is an essential prelude to a structure determination, but for many viral and human proteins this cannot be achieved using prokaryotic expression systems. Groups in the Structural Proteomics In Europe (SPINE) consortium have developed and implemented high-throughput (HTP) methodologies for cloning, expression screening and protein production in eukaryotic systems. Studies focused on three systems: yeast (Pichia pastoris and Saccharomyces cerevisiae), baculovirus-infected insect cells and transient expression in mammalian cells. Suitable vectors for HTP cloning are described and results from their use in expression screening and protein-production pipelines are reported. Strategies for co-expression, selenomethionine labelling (in all three eukaryotic systems) and control of glycosylation (for secreted proteins in mammalian cells) are assessed. © International Union of Crystallography, 2006.

Original language	English
Pages (from-to)	1114-1124
Number of pages	11
Journal	Acta Crystallographica: Section D
Volume	62
Issue number	10
DOIs	https://doi.org/10.1107/S0907444906029805
Publication status	Published - Oct 2006

Bibliographical note

Copyright © International Union of Crystallography
Author(s) of this paper may load this reprint on their own web site or institutional repository provided that
this cover page is retained. Republication of this article or its storage in electronic databases other than as
specified above is not permitted without prior permission in writing from the IUCr.

Keywords

baculovirus
eukaryotic expression
high throughput
mammalian cells
yeast

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Aricescu, A. R., Assenberg, R., Bill, R. M., Busso, D., Chang, V. T., Davis, S. J., Dubrovsky, A., Gustafsson, L., Hedfalk, K., Heinemann, U., Jones, I. M., Ksiazek, D., Lang, C., Maskos, K., Messerschmidt, A., Macieira, S., Peleg, Y., Perrakis, A., Poterszman, A., ... Jones, E. Y. (2006). Eukaryotic expression: Developments for structural proteomics. Acta Crystallographica: Section D, 62(10), 1114-1124. https://doi.org/10.1107/S0907444906029805

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note = "Copyright {\textcopyright} International Union of Crystallography Author(s) of this paper may load this reprint on their own web site or institutional repository provided that this cover page is retained. Republication of this article or its storage in electronic databases other than as specified above is not permitted without prior permission in writing from the IUCr. ",

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Aricescu, AR, Assenberg, R, Bill, RM, Busso, D, Chang, VT, Davis, SJ, Dubrovsky, A, Gustafsson, L, Hedfalk, K, Heinemann, U, Jones, IM, Ksiazek, D, Lang, C, Maskos, K, Messerschmidt, A, Macieira, S, Peleg, Y, Perrakis, A, Poterszman, A, Schneider, G, Sixma, TK, Sussman, JL, Sutton, G, Tarboureich, N, Zeev-Ben-Mordehai, T & Jones, EY 2006, 'Eukaryotic expression: Developments for structural proteomics', Acta Crystallographica: Section D, vol. 62, no. 10, pp. 1114-1124. https://doi.org/10.1107/S0907444906029805

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T1 - Eukaryotic expression

T2 - Developments for structural proteomics

AU - Aricescu, A.R.

AU - Assenberg, R.

AU - Bill, Roslyn M.

AU - Busso, D.

AU - Chang, V.T.

AU - Davis, S.J.

AU - Dubrovsky, A.

AU - Gustafsson, L.

AU - Hedfalk, K.

AU - Heinemann, U.

AU - Jones, I.M.

AU - Ksiazek, D.

AU - Lang, C.

AU - Maskos, K.

AU - Messerschmidt, A.

AU - Macieira, S.

AU - Peleg, Y.

AU - Perrakis, A.

AU - Poterszman, A.

AU - Schneider, G.

AU - Sixma, T.K.

AU - Sussman, J.L.

AU - Sutton, G.

AU - Tarboureich, N.

AU - Zeev-Ben-Mordehai, T.

AU - Jones, E. Yvonne

N1 - Copyright © International Union of Crystallography Author(s) of this paper may load this reprint on their own web site or institutional repository provided that this cover page is retained. Republication of this article or its storage in electronic databases other than as specified above is not permitted without prior permission in writing from the IUCr.

PY - 2006/10

Y1 - 2006/10

N2 - The production of sufficient quantities of protein is an essential prelude to a structure determination, but for many viral and human proteins this cannot be achieved using prokaryotic expression systems. Groups in the Structural Proteomics In Europe (SPINE) consortium have developed and implemented high-throughput (HTP) methodologies for cloning, expression screening and protein production in eukaryotic systems. Studies focused on three systems: yeast (Pichia pastoris and Saccharomyces cerevisiae), baculovirus-infected insect cells and transient expression in mammalian cells. Suitable vectors for HTP cloning are described and results from their use in expression screening and protein-production pipelines are reported. Strategies for co-expression, selenomethionine labelling (in all three eukaryotic systems) and control of glycosylation (for secreted proteins in mammalian cells) are assessed. © International Union of Crystallography, 2006.

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KW - baculovirus

KW - eukaryotic expression

KW - high throughput

KW - mammalian cells

KW - yeast

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U2 - 10.1107/S0907444906029805

DO - 10.1107/S0907444906029805

M3 - Article

C2 - 17001089

SN - 0907-4449

VL - 62

SP - 1114

EP - 1124

JO - Acta Crystallographica: Section D

JF - Acta Crystallographica: Section D

IS - 10

ER -

Eukaryotic expression: Developments for structural proteomics

Abstract

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Keywords

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