19962020

Research output per year

If you made any changes in Pure these will be visible here soon.

Personal profile

Research Interests

I lead AMPL, a collaborative, multidisciplinaryteam of six principal investigators, who work on membrane proteins and lipids at Aston. Membrane proteins play pivotal roles in all cellular functions including the transport of nutrients, the export of toxins and cell-cell interactions. They are also ubiquitous: ~30% of all proteins in all cells are membrane proteins. Consequently they are already key drug targets, with the majority of prescription pharmaceuticals on the market being targeted to this class of protein. Unfortunately, however, they are neither naturally abundant nor stable, which means that in the vast majority of cases extracting them from natural sources is not a viable option in order to study their structures, functions and biochemistry.

A main theme of our research activity is developing yeast as a “cell factory” to synthesize recombinant membrane proteins in sufficient quantity and of appropriate quality for further study. Our approach is to move away from “trial and error” and to rationalize recombinant protein production by understanding the host cell response to it.  We aim to increase the volumetric yield, increase the yield “per cell” and understand the molecular mechanisms involved.

We work on a range of membrane proteins. One of our projects examines in detail the regulation of the aquaporin water channel proteins. Aquaporins form one of the protein families we produce in our yeast cell factories and are of interest as novel drug targets. We recently showed that AQP1 is regulated by extracellular water availability (Biochemistry. 2010, 49:821-3). This work was featured on the front cover of its issue, and as part of the 50th birthday cover. We also work on tetraspanins, especially CD81, as well as a range of GPCRs.

We acknowledge EPSRC, BBSRC, the Technology Strategy Board, AstraZeneca Ltd, Alpha Biologics Ltd, Applikon Biotechnology, Johnson & Johnson, Glycoform Ltd, Polytherics Ltd and the European Commission for their support. We have co-ordinated three Framework Programme 6 projects: European Membrane Protein Consortium (E-MeP), E-MeP-Lab and OptiCryst. We are also a partner of the Framework Programme 7 EDICT project.

See my profile on ResearchGate: https://www.researchgate.net/profile/Roslyn_Bill

Follow me on Twitter: @RoslynBill

 

Employment

Administrative Roles

Associate Dean (Research) for the School of Life and Health Sciences
Member of the Biosciences Research Group; Cellular and Molecular Biomedicine
Member - Aston Research Centre for Healthy Ageing and Aston Membrane Proteins and Lipids

Contact Details

email: r.m.bill@aston.ac.uk
telephone:  +44 121 204 4274  
fax: +44 121 204 4175

External positions

Chair, BBSRC Research Committee E

External Examiner MSc Industrial Biotechnology, University of York

Keywords

  • QH301 Biology
  • Yeast, membrane proteins, aquaporin, tetraspanin, GPCR

Fingerprint Dive into the research topics where Roslyn Bill is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

  • 3 Similar Profiles

Network Recent external collaboration on country level. Dive into details by clicking on the dots.

Research Output

Expression and purification of recombinant G protein-coupled receptors: A review

Wiseman, D. N., Otchere, A., Patel, J. H., Uddin, R., Pollock, N. L., Routledge, S. J., Rothnie, A. J., Slack, C., Poyner, D. R., Bill, R. M. & Goddard, A. D., 1 Mar 2020, In : Protein Expression and Purification. 167, 105524.

Research output: Contribution to journalArticle

Open Access
File
  • Ligand-induced conformational changes in a SMALP-encapsulated GPCR.

    Routledge, S. J., Jamshad, M., Little, H. A., Lin, Y. P., Simms, J., Thakker, A., Spickett, C. M., Bill, R. M., Dafforn, T. R., Poyner, D. R. & Wheatley, M., 29 Feb 2020, In : Biochimica et Biophysica Acta - Biomembranes. 1862, 6, 183235.

    Research output: Contribution to journalArticle

    Open Access
    File
  • Analyzing recombinant protein production in Pichia pastoris with targeted proteomics

    Bill, R. M., Ulke-Lemée, A., Cartwright, S. P., Far, R., Kim, J. & MacDonald, J. A., 9 Feb 2019, Recombinant Protein Production in Yeast. Gasser, B. & Mattanovich, D. (eds.). Humana Press, Vol. 1923. p. 187-209 23 p. (Methods in Molecular Biology; vol. 1923).

    Research output: Chapter in Book/Report/Conference proceedingChapter

  • Functional expression of Multidrug Resistance Protein 4 MRP4/ABCC4

    Hardy, D., Bill, R. M., Jawhari, A. & Rothnie, A. J., 1 Dec 2019, In : SLAS Discovery. 24, 10, p. 1000-1008 9 p.

    Research output: Contribution to journalArticle

    Open Access
    File
  • Inhibitors of Mammalian Aquaporin Water Channels

    Abir-Awan, M., Kitchen, P., Salman, M. M., Conner, M. T., Conner, A. C. & Bill, R. M., 29 Mar 2019, In : International Journal of Molecular Sciences. 20, 7, 1589.

    Research output: Contribution to journalReview article

    Open Access
    File
  • Thesis

    ATP-binding cassette subfamily C (ABCC) transporter 1 (ABCC1) and 4 (ABCC4) independent of their drug efflux ability affects breast cancer biology

    Author: Low, F., 15 May 2019

    Supervisor: Rothnie, A. (Supervisor) & Bill, R. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

    File

    Engineering yeast for G protein-coupled receptor functional studies: pharmacological characterisation of adrenergic receptors

    Author: Mikaliunaite, L., 1 Mar 2018

    Supervisor: Bill, R. M. (Supervisor) & Dowell, S. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

    File

    Improved production of industrially relevant recombinant proteins: application of multi-factorial design of experiments and scalable process modelling

    Author: Holmes, W., Feb 2009

    Supervisor: Bill, R. M. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

    File

    Improving recombinant human adenosine A2A receptor production in yeast

    Author: Bawa, Z., 25 Jun 2014

    Supervisor: Bill, R. M. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

    File

    Investigation of the activation mechanism and structural characterization of the CGRP receptor

    Author: Kuteyi, G., 6 Dec 2013

    Supervisor: Poyner, D. R. (Supervisor) & Bill, R. M. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

    File

    Activities

    • 5 Editorial activity
    • 2 Examination

    External Examiner MSc Molecular Biotechnology

    Roslyn Bill (Examiner)
    2015 → …

    Activity: Examination typesExamination

    Current Opinion in Structural Biology (Journal)

    Roslyn Bill (Guest editor)
    2015

    Activity: Publication peer-review and editorial work typesEditorial activity

    Microbial Cell Factories (Journal)

    Roslyn Bill (Editorial board member)
    2014

    Activity: Publication peer-review and editorial work typesEditorial activity

    Molecular Membrane Biology (Journal)

    Roslyn Bill (Guest editor)
    2011 → …

    Activity: Publication peer-review and editorial work typesEditorial activity

    Biotechnology Letters (Journal)

    Roslyn Bill (Editorial board member)
    2010 → …

    Activity: Publication peer-review and editorial work typesEditorial activity