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Personal profile

Research Interests

I lead AMPL, a collaborative, multidisciplinaryteam of six principal investigators, who work on membrane proteins and lipids at Aston. Membrane proteins play pivotal roles in all cellular functions including the transport of nutrients, the export of toxins and cell-cell interactions. They are also ubiquitous: ~30% of all proteins in all cells are membrane proteins. Consequently they are already key drug targets, with the majority of prescription pharmaceuticals on the market being targeted to this class of protein. Unfortunately, however, they are neither naturally abundant nor stable, which means that in the vast majority of cases extracting them from natural sources is not a viable option in order to study their structures, functions and biochemistry.

A main theme of our research activity is developing yeast as a “cell factory” to synthesize recombinant membrane proteins in sufficient quantity and of appropriate quality for further study. Our approach is to move away from “trial and error” and to rationalize recombinant protein production by understanding the host cell response to it.  We aim to increase the volumetric yield, increase the yield “per cell” and understand the molecular mechanisms involved.

We work on a range of membrane proteins. One of our projects examines in detail the regulation of the aquaporin water channel proteins. Aquaporins form one of the protein families we produce in our yeast cell factories and are of interest as novel drug targets. We recently showed that AQP1 is regulated by extracellular water availability (Biochemistry. 2010, 49:821-3). This work was featured on the front cover of its issue, and as part of the 50th birthday cover. We also work on tetraspanins, especially CD81, as well as a range of GPCRs.

We acknowledge EPSRC, BBSRC, the Technology Strategy Board, AstraZeneca Ltd, Alpha Biologics Ltd, Applikon Biotechnology, Johnson & Johnson, Glycoform Ltd, Polytherics Ltd and the European Commission for their support. We have co-ordinated three Framework Programme 6 projects: European Membrane Protein Consortium (E-MeP), E-MeP-Lab and OptiCryst. We are also a partner of the Framework Programme 7 EDICT project.

See my profile on ResearchGate: https://www.researchgate.net/profile/Roslyn_Bill

Follow me on Twitter: @RoslynBill

 

Employment

Administrative Roles

Associate Dean (Research) for the School of Life and Health Sciences
Member of the Biosciences Research Group; Cellular and Molecular Biomedicine
Member - Aston Research Centre for Healthy Ageing and Aston Membrane Proteins and Lipids

Contact Details

email: r.m.bill@aston.ac.uk
telephone:  +44 121 204 4274  
fax: +44 121 204 4175

External positions

Chair, BBSRC Research Committee E

External Examiner MSc Industrial Biotechnology, University of York

Keywords

  • QH301 Biology
  • Yeast, membrane proteins, aquaporin, tetraspanin, GPCR

Fingerprint Dive into the research topics where Roslyn Bill is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

  • 3 Similar Profiles
Yeasts Medicine & Life Sciences
Recombinant Proteins Medicine & Life Sciences
Yeast Chemical Compounds
Membrane Proteins Medicine & Life Sciences
Aquaporins Medicine & Life Sciences
Pichia Medicine & Life Sciences
Saccharomyces cerevisiae Medicine & Life Sciences
Proteins Medicine & Life Sciences

Network Recent external collaboration on country level. Dive into details by clicking on the dots.

Research Output 1996 2020

Analyzing recombinant protein production in Pichia pastoris with targeted proteomics

Bill, R. M., Ulke-Lemée, A., Cartwright, S. P., Far, R., Kim, J. & MacDonald, J. A., 9 Feb 2019, Recombinant Protein Production in Yeast. Gasser, B. & Mattanovich, D. (eds.). Humana Press, Vol. 1923. p. 187-209 23 p. (Methods in Molecular Biology; vol. 1923).

Research output: Chapter in Book/Report/Conference proceedingChapter

Pichia
Recombinant Proteins
Proteomics
Mass Spectrometry
Antibodies

Expression and purification of recombinant G protein-coupled receptors: A review

Wiseman, D. N., Otchere, A., Patel, J. H., Uddin, R., Pollock, N. L., Routledge, S. J., Rothnie, A. J., Slack, C., Poyner, D. R., Bill, R. M. & Goddard, A. D., 31 Oct 2019, In : Protein Expression and Purification. 167, 105524.

Research output: Contribution to journalArticle

Open Access
File
Drug Design
Drug Discovery
G-Protein-Coupled Receptors
Recombinant Proteins
Detergents

Functional expression of Multidrug Resistance Protein 4 MRP4/ABCC4

Hardy, D., Bill, R. M., Jawhari, A. & Rothnie, A. J., 1 Dec 2019, In : SLAS Discovery. 24, 10, p. 1000-1008 9 p.

Research output: Contribution to journalArticle

Open Access
File
P-Glycoproteins
Adenosine Triphosphate
Sf9 Cells
Membrane Proteins
Insects

Inhibitors of Mammalian Aquaporin Water Channels

Abir-Awan, M., Kitchen, P., Salman, M. M., Conner, M. T., Conner, A. C. & Bill, R. M., 29 Mar 2019, In : International Journal of Molecular Sciences. 20, 7, 1589.

Research output: Contribution to journalReview article

Open Access
File
Aquaporins
inhibitors
acetazolamide
pathology
metastasis

Lignocellulosic ethanol production: Evaluation of new approaches, cell immobilization and reactor configurations

Karagoz, P., Bill, R. M. & Ozkan, M., 1 Dec 2019, In : Renewable energy. 143, p. 741-752 12 p.

Research output: Contribution to journalReview article

Cell immobilization
Ethanol
Cellulosic ethanol
Biomass
Metabolic engineering

Thesis

Engineering yeast for G protein-coupled receptor functional studies: pharmacological characterisation of adrenergic receptors

Author: Mikaliunaite, L., 1 Mar 2018

Supervisor: Bill, R. M. (Supervisor) & Dowell, S. (Supervisor)

Student thesis: Doctoral ThesisDoctor of Philosophy

File

Improved production of industrially relevant recombinant proteins: application of multi-factorial design of experiments and scalable process modelling

Author: Holmes, W., Feb 2009

Supervisor: Bill, R. M. (Supervisor)

Student thesis: Doctoral ThesisDoctor of Philosophy

File

Improving recombinant human adenosine A2A receptor production in yeast

Author: Bawa, Z., 25 Jun 2014

Supervisor: Bill, R. M. (Supervisor)

Student thesis: Doctoral ThesisDoctor of Philosophy

File

Investigation of the activation mechanism and structural characterization of the CGRP receptor

Author: Kuteyi, G., 6 Dec 2013

Supervisor: Poyner, D. R. (Supervisor) & Bill, R. M. (Supervisor)

Student thesis: Doctoral ThesisDoctor of Philosophy

File

Novel strategies to improve recombinant membrane protein production in yeast

Author: Dilworth, M., 2 Aug 2014

Supervisor: Bill, R. M. (Supervisor) & Gross, S. R. (Supervisor)

Student thesis: Doctoral ThesisDoctor of Philosophy

File

Activities 2007 2015

  • 5 Editorial activity
  • 2 Examination

Current Opinion in Structural Biology (Journal)

Roslyn Bill (Guest editor)
2015

Activity: Publication peer-review and editorial work typesEditorial activity

External Examiner MSc Molecular Biotechnology

Roslyn Bill (Examiner)
2015 → …

Activity: Examination typesExamination

Microbial Cell Factories (Journal)

Roslyn Bill (Editorial board member)
2014

Activity: Publication peer-review and editorial work typesEditorial activity

Molecular Membrane Biology (Journal)

Roslyn Bill (Guest editor)
2011 → …

Activity: Publication peer-review and editorial work typesEditorial activity

Molecular Biotechnology (Journal)

Roslyn Bill (Editorial board member)
2010 → …

Activity: Publication peer-review and editorial work typesEditorial activity