Glycogen, the preferred energy source for extracellular protein formation and growth of Aeromonas salmonicida

Geoffrey Coleman, Russell Collighan, Stephen Dodsworth

Research output: Contribution to journalArticle

Abstract

A study was made of the effect of supplementing a rich 3% (w/v) tryptone soya broth (TSB) medium and a poorer 1.7% (w/v) tryptone-based medium with glucose, maltose and glycogen, as carbon sources, on growth and exoprotein formation by Aeromonas salmonicida. In TSB, glucose inhibited growth and repressed exoprotein formation whilst maltose and glycogen had little effect, up to 20 h, when compared with an unsupplemented control. By contrast, in the poorer medium, over a 24-h incubation period, growth was stimulated three-fold by glycogen, and whilst exoprotein formation was low in comparison with that observed in TSB, the greatest production was observed in the presence of glycogen. Extracellular alpha-amylase was measured in the tryptone medium in the presence of the three carbon sources and the highest level, produced in the presence of glycogen, was 1.6 times that with added maltose whilst none was detectable with glucose present. This pattern was repeated in the case of the maltose-inducible porin, LamB, of the outer membrane.
Original languageEnglish
Pages (from-to)221-228
Number of pages8
JournalJournal of Fish Diseases
Volume18
Issue number3
DOIs
Publication statusPublished - May 1995

Keywords

  • tryptone soya broth
  • aeromonas salmonicida
  • supplement

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