Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification

Nicola McMillan; Louise V Smith; Jesus M de la Fuente; Alexis D C Parenty; Nikolaj Gadegaard; Andrew R Pitt; Katrina Thomson; Cameron Mackenzie; Sharon M Kelly; Leroy Cronin

doi:10.1039/b702575k

Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification

Nicola McMillan, Louise V Smith, Jesus M de la Fuente, Alexis D C Parenty, Nikolaj Gadegaard, Andrew R Pitt, Katrina Thomson, Cameron Mackenzie, Sharon M Kelly, Leroy Cronin

Research output: Contribution to journal › Article › peer-review

Abstract

N-Heterocyclic cations are incorporated into proteins using 5-(2-bromoethyl)phenanthridinium bromide, which selectively reacts with either cysteine or lysine residues, resulting in ethylphenanthridinium (Phen) or highly stable cyclised dihydro-imidazo-phenanthridinium (DIP) adducts respectively; these modifications have been found to manipulate the observed structure of lysozyme and bovine serum albumin by AFM.

Original language	English
Pages (from-to)	2581-3258
Number of pages	3
Journal	Chemical Communications
Volume	2007
Issue number	25
DOIs	https://doi.org/10.1039/b702575k
Publication status	Published - May 2007

Keywords

animals
cell membrane
chickens
egg white
fluorescein-5-isothiocyanate
heterocyclic compounds
atomic force microscopy
muramidase
proteins
bovine serum albumin

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10.1039/b702575k

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title = "Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification",

abstract = "N-Heterocyclic cations are incorporated into proteins using 5-(2-bromoethyl)phenanthridinium bromide, which selectively reacts with either cysteine or lysine residues, resulting in ethylphenanthridinium (Phen) or highly stable cyclised dihydro-imidazo-phenanthridinium (DIP) adducts respectively; these modifications have been found to manipulate the observed structure of lysozyme and bovine serum albumin by AFM.",

keywords = "animals, cell membrane, chickens, egg white, fluorescein-5-isothiocyanate, heterocyclic compounds, atomic force microscopy, muramidase, proteins, bovine serum albumin",

author = "Nicola McMillan and Smith, {Louise V} and {de la Fuente}, {Jesus M} and Parenty, {Alexis D C} and Nikolaj Gadegaard and Pitt, {Andrew R} and Katrina Thomson and Cameron Mackenzie and Kelly, {Sharon M} and Leroy Cronin",

year = "2007",

month = may,

doi = "10.1039/b702575k",

language = "English",

volume = "2007",

pages = "2581--3258",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "25",

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TY - JOUR

T1 - Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification

AU - McMillan, Nicola

AU - Smith, Louise V

AU - de la Fuente, Jesus M

AU - Parenty, Alexis D C

AU - Gadegaard, Nikolaj

AU - Pitt, Andrew R

AU - Thomson, Katrina

AU - Mackenzie, Cameron

AU - Kelly, Sharon M

AU - Cronin, Leroy

PY - 2007/5

Y1 - 2007/5

N2 - N-Heterocyclic cations are incorporated into proteins using 5-(2-bromoethyl)phenanthridinium bromide, which selectively reacts with either cysteine or lysine residues, resulting in ethylphenanthridinium (Phen) or highly stable cyclised dihydro-imidazo-phenanthridinium (DIP) adducts respectively; these modifications have been found to manipulate the observed structure of lysozyme and bovine serum albumin by AFM.

AB - N-Heterocyclic cations are incorporated into proteins using 5-(2-bromoethyl)phenanthridinium bromide, which selectively reacts with either cysteine or lysine residues, resulting in ethylphenanthridinium (Phen) or highly stable cyclised dihydro-imidazo-phenanthridinium (DIP) adducts respectively; these modifications have been found to manipulate the observed structure of lysozyme and bovine serum albumin by AFM.

KW - animals

KW - cell membrane

KW - chickens

KW - egg white

KW - fluorescein-5-isothiocyanate

KW - heterocyclic compounds

KW - atomic force microscopy

KW - muramidase

KW - proteins

KW - bovine serum albumin

UR - http://pubs.rsc.org/en/Content/ArticleLanding/2007/CC/b702575k

U2 - 10.1039/b702575k

DO - 10.1039/b702575k

M3 - Article

C2 - 17579744

SN - 1359-7345

VL - 2007

SP - 2581

EP - 3258

JO - Chemical Communications

JF - Chemical Communications

IS - 25

ER -

Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification

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Keywords

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