Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification

Nicola McMillan, Louise V Smith, Jesus M de la Fuente, Alexis D C Parenty, Nikolaj Gadegaard, Andrew R Pitt, Katrina Thomson, Cameron Mackenzie, Sharon M Kelly, Leroy Cronin

Research output: Contribution to journalArticle

Abstract

N-Heterocyclic cations are incorporated into proteins using 5-(2-bromoethyl)phenanthridinium bromide, which selectively reacts with either cysteine or lysine residues, resulting in ethylphenanthridinium (Phen) or highly stable cyclised dihydro-imidazo-phenanthridinium (DIP) adducts respectively; these modifications have been found to manipulate the observed structure of lysozyme and bovine serum albumin by AFM.
Original languageEnglish
Pages (from-to)2581-3258
Number of pages3
JournalChemical Communications
Volume2007
Issue number25
DOIs
Publication statusPublished - May 2007

Keywords

  • animals
  • cell membrane
  • chickens
  • egg white
  • fluorescein-5-isothiocyanate
  • heterocyclic compounds
  • atomic force microscopy
  • muramidase
  • proteins
  • bovine serum albumin

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    McMillan, N., Smith, L. V., de la Fuente, J. M., Parenty, A. D. C., Gadegaard, N., Pitt, A. R., Thomson, K., Mackenzie, C., Kelly, S. M., & Cronin, L. (2007). Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification. Chemical Communications, 2007(25), 2581-3258. https://doi.org/10.1039/b702575k