We report the simplification and development of biofunctionalization methodology based on one-step 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)-mediated reaction. The dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a standard LPG sensor used in low RI range. With the simple and efficient immobilization of unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM with the advantages of label-free, real-time, and ultrahigh sensitivity.
|Title of host publication||Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI|
|Place of Publication||Bellingham, WA (US)|
|Number of pages||6|
|Publication status||Published - 7 Mar 2016|
|Event||SPIE Photonics West BiOS 2016 - The Moscone Center, San Francisco, United States|
Duration: 13 Feb 2016 → 18 Feb 2016
|Conference||SPIE Photonics West BiOS 2016|
|Period||13/02/16 → 18/02/16|
Bibliographical noteXianfeng Chen ; Chen Liu ; Marcos R. Cardoso ; Cleber R. Mendonça ; David A. Nagel ; Anna V. Hine and Lin Zhang; "Label-free oligonucleotide biosensor based on dual-peak long period fiber grating", Proc. SPIE 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI, 97020S (March 7, 2016).
Copyright 2016. Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
- EDC-mediated reaction
- long period grating
- optical fiber biosensor