Label-free oligonucleotide biosensor based on dual-peak long period fiber grating

Xianfeng Chen, Chen Liu, Marcos R. Cardoso, Cleber R. Mendonça, David A. Nagel, Anna V. Hine, Lin Zhang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report the simplification and development of biofunctionalization methodology based on one-step 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)-mediated reaction. The dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a standard LPG sensor used in low RI range. With the simple and efficient immobilization of unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM with the advantages of label-free, real-time, and ultrahigh sensitivity.

Original languageEnglish
Title of host publicationOptical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI
EditorsIsrael Gannot
Place of PublicationBellingham, WA (US)
PublisherSPIE
Number of pages6
ISBN (Print)978-1-6284-1936-8
DOIs
Publication statusPublished - 7 Mar 2016
EventSPIE Photonics West BiOS 2016 - The Moscone Center, San Francisco, United States
Duration: 13 Feb 201618 Feb 2016

Publication series

NameSPIE Proceedings
PublisherSPIE
Volume9702
ISSN (Print)0277-786X
ISSN (Electronic)2410-9045

Conference

ConferenceSPIE Photonics West BiOS 2016
CountryUnited States
CitySan Francisco
Period13/02/1618/02/16

Bibliographical note

Xianfeng Chen ; Chen Liu ; Marcos R. Cardoso ; Cleber R. Mendonça ; David A. Nagel ; Anna V. Hine and Lin Zhang; "Label-free oligonucleotide biosensor based on dual-peak long period fiber grating", Proc. SPIE 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI, 97020S (March 7, 2016).
Copyright 2016. Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
DOI: http://dx.doi.org/10.1117/12.2207869

Keywords

  • DNA
  • EDC-mediated reaction
  • label-free
  • long period grating
  • oligonucleotide
  • optical fiber biosensor

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  • Cite this

    Chen, X., Liu, C., Cardoso, M. R., Mendonça, C. R., Nagel, D. A., Hine, A. V., & Zhang, L. (2016). Label-free oligonucleotide biosensor based on dual-peak long period fiber grating. In I. Gannot (Ed.), Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI [97020S] (SPIE Proceedings; Vol. 9702). SPIE. https://doi.org/10.1117/12.2207869